Application of Red Dragon Fruit Dyes as Staining in Histological Study

  • Ridho Asra School of Pharmaceutical Science Padang, West Sumatera, Indonesia
  • Rusdi R Schoolof Pharmaceutical Science Padang, West Sumatera, Indonesia
  • Indah Kemala Fitrina School of Pharmaceutical Science Padang, West Sumatera, Indonesia
  • Nessa Nessa Perintis of University, Padang, West Sumatera, Indonesia

Abstract

Background: The betacyanin pigment found in dragon fruit flesh (Hylocereus costaricensis (F.A.C.Weber) Britton & Rose) has many advantages, for instance as a tissue histology dye.


Objectives: determining the properties of organs stained with betacyanin red dye and finding alternative histology colors to replace synthetic hematoxylin-eosin dyes.


Summary of contents of the article: The flesh of the red dragon fruit (Hylocereus costaricensis (F.A.C.Weber) Britton & Rose) was extracted using the Ultrasonic Assisted Extraction (UAE) method. The study employed six male white mice divided into two treatment groups: a placebo group induced with 1% Na.CMC carrier solution and a positive control group generated a toxic dose of paracetamol. The results were visually compared to hematoxylin-eosin with standard betacyanin and betacyanin extract of red dragon fruit flesh concentrations of 50% (v/v), 80% (v/v), and 100% (v/v).


Conclusion: Betacyanin from the flesh of the red dragon fruit can be applied as a histological dye in the liver of male white mice and as a counterstaining hematoxylin as a substitute for eosin dye at a concentration of 100% (v/v).


 

Keywords: Red dragon fruit, Betacyanin, Histology

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Author Biographies

Ridho Asra, School of Pharmaceutical Science Padang, West Sumatera, Indonesia

School of Pharmaceutical Science Padang, West Sumatera, Indonesia

Rusdi R, Schoolof Pharmaceutical Science Padang, West Sumatera, Indonesia

Schoolof Pharmaceutical Science Padang, West Sumatera, Indonesia

Indah Kemala Fitrina, School of Pharmaceutical Science Padang, West Sumatera, Indonesia

School of Pharmaceutical Science Padang, West Sumatera, Indonesia

Nessa Nessa, Perintis of University, Padang, West Sumatera, Indonesia

Perintis of University, Padang, West Sumatera, Indonesia

References

1. Harivandaran, K. V., Rebecca, O. P., & Chandran, S. Study of Optimal Temperature, pH, And Stability of Dragon Fruit (Hylocereus polyrhizus) Peel for Use as Potential Natural Colorant. Pakistan Journal of Biological Sciences, 2008; 11(18):2259-2263.
2. Lim, T. K. (2012). Hylocereus polyrhizus. In Edible Medicinal and Non-Medicinal. Berlin: Springer.
3. Wybraniec, S. I., & Platzner, S. G. (2001). Betacyanins from Vine Cactus Hylocereus polyrhizus. Phytochemistry, 2001; 58(8):1209–1212.
4. Asra, R., Yetti, R.D., Misfadhila, S., Audina, S., Agustina, A., Nessa. Aktivitas Antioksidan dari Ekstrak Kering Kulit dan Daging Buah Naga (Hylocereus lemairei (Hook) Britton & Rose). Jurnal Farmasi Higea, 2019; 11(1):17-22.
5. Wu, L., Hsu, H., Chen, Y., Chiu, C., Lin, Y., & Ho, J. A. Antioxidant and Antiproliferative Actives of Red Pitaya. Food Chemistry. 2006; 95:319-327.
6. Wybraniec, S., & Mizrahi, Y. (2002). Fruit Flesh Betacyanin Pigments in Hylocereus cacti. Journal of Agricultural and Food Chemistry, 50, 6086–6089.
7. Herbach, K. M., Stinizing, F. C., Carle, R. (2006). Betalain Stability and Degradation Structural and Chromatic Aspects. Journal of food science. 2006; 71(4):41-50.
8. Agne, E. B. P., Hastuti, R., & Khabibi. (2010). Ekstraksi dan Uji Kestabilan Zat Warna Betasianin dari Kulit Buah Naga (Hylocereus polyrhizus) serta Aplikasinya sebagai Pewarna Alami Pangan. Jurnal Kimia Sains dan Aplikasi,2010; 13(2):1410-1417.
9. Allam, K. V., Kumar, G. Colorants − the Cosmetics for the Pharmaceutical Dosage forms. International Journal of Pharmacy and Pharmaceutical Sciences, 2011; 3(1), 13-21.
10. Leeson, C. R., Leeson, T. S., & Paparo, A. A. (1996). Buku Ajar Histologi. (Edisi 5). Penerjemah: Y. Tambayong. Jakarta: Penerbit Buku Kedokteran EGC.
11. Ellywati. (2018). Penetuan Waktu Yang Tepat pada Proses Staining dalam Pembuatan Preparat Histologis Hati. Journal on Biology of Andalas University (JbioUA),2018; 1(1):28-30.
12. Sigh, K. 2002. Syarat-syarat Standar Zat Warna Ideal. Theory and Practice of Histological Techniques, 4(2), 230-238.
13. Kuncorojakti, S. Evaluasi Pewarnaan Toluidine Blue untuk Identifikasi Sel Mast Jaringan Ikat dari Preparat Blok Parafin Kulit Tipis Anjing. Veterinaria Medika, 2014; 7(2):120-125.
14. Wulandari, F. Y. S., Widiyani, S. D., & Iswara, A. (2019). Caesar (Caesalpinia Extract) : Pewarna Alami Tanaman Indonesia Pengganti Giemsa. Jurnal Labora Medika, 2019; 3:45-49.
15. Safitry, A. I. (2019). Studi Fisikokimia Betasianin Dari Daging Buah Naga Merah (Hylocereus lemairei (Hook.) Britton & Rose) Serta Aplikasinya Sebagai Tracking Dye Untuk Elektroforesis Gel Agarose. (Skripsi). Padang: Sekolah Tinggi Ilmu Farmasi.
16. Sumaryani, N. P., & Dharmadewi, A. A. I. M. Analisis Kandungan Vitamin C Buah Naga Merah (Hylocereus polyrhicuz) dan Buah Naga Putih (Hylocereus undatus) pada Penyimpanan dengan Suhu Dan Waktu yang Berbeda. Jurnal Metamorfosa. 2018; 5(2), 249-253.
17. Ajileye, A. B., Itere, A. K., & Arigi, Q. B. Zingiber Officinale (Ginger) Extract as a Histological Dye For Muscle Fibers and Cytoplasm. International Journal of Medical Science and Public Health, 2015; 4(10):1445-1448.
18. Shehu, S. A., Sonfada, M. L., Danmaigoro, A., Umar, A. A., Hena, S. A., & Wiam, I. M. Kola Nut (Cola acuminata) Extract as a Substitute to Histological Tissue Stain Eosin. Scientific Journal of Veterinary Advances, 2012; 1(2):33-37.
19. Jusuf, A. A. (2009). Histoteknik Dasar. Jakarta: Histologi Fakultas Kedokteran Universitas Indonesia.
20. Mescher, A. L. (2016). Junqueira’s Basic Histology Text and Atlas. (14th Edition). United States: McGraw-Hill Education.
21. Suvarna, S. K., Layton, C., & Bancroft, J. D. (2013). Bancroft's Theory and Practice Of Histological Techniques. (7th Edition). London: Churchill Livingstone Elsevier.
22. Muthi’ah, W. Eksplorasi Teknik Pewarnaan Alam dengan Ekstrak Kayu Jambal pada Batik Kayu Gempol. Jurnal Narada,2019; 6(2):313-328.
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How to Cite
Asra, R., R, R., Fitrina, I., & Nessa, N. (2021). Application of Red Dragon Fruit Dyes as Staining in Histological Study. Asian Journal of Pharmaceutical Research and Development, 9(6), 48-55. https://doi.org/https://doi.org/10.22270/ajprd.v9i6.1045