Isolation and identification of flavonoids from avocado leaves (Persea americana Mill)
Leaves of avocado (Persea americana Mill) contains bioactive compounds that can act as natural antioxidants. This study was conducted to isolate and identify the flavonoid compounds of avocado leaves. Research stages includes maceration, fractionation, thin-layer chromatography analysis, isolation and purification of compounds also identification of isolates in two-dimensional thin-layer chromatography and by using three different types of solvents. Isolates were identified using ultraviolet-visible spectrophotometry and infrared spectrophotometry. From the results obtained one isolate FA6 showed a single compound. Based on the results by testing the purity of isolates using two-dimensional thin-layer chromatography and by using three different types of solvents, isolates FA6 is a pure compound. Identification by using ray-visible spectrophotometry FA6 isolates showed maximum wavelength at 280.6 nm and 342.0 nm on bands II and on band I. Infrared spectrum ofisolate FA6 showed aromatic OH groups, aliphatic CH, C = O stretching, C = C aromatic, and C-O-C stretch. From the results obtained isolates FA6 is a class of flavonoids group flavones or flavonol (3-OH substitional).
2. Yuniarti T. Ensiklopedia Tanaman Obat Tradisional. Yogyakarta: MedPress. 2008
3. Adeyemi OO, Okpo SO and Ogunti OO. 2002.Analgesics and anti-inflammatory effect of leaves of Persea Americana Mill (Lauraceae).Fitoterapia 2002; 73:375-380.
4. Owolabi MA, Coker HAB, and Jaja SI. 2010. Bioactivity of The Phytoconstituents of the leaves of Persea Americana. Nigeria. Journal of Medicinal Plants Research 2010; 4(12):1130-5.
5. Arukwe U, Amadi BA, Duru MKC, Agomuo EN, Adindu EA, Odika PC. Chemical Composition of Persea Americana Leaf, Fruit and Seed.Nigeria. 2012:11 ISSUE2/IJRRAS 11.
6. Markham KR. Flavonoids identification (Indonesian: Cara Mengidentifikasi Flavonoid). Penerjemah. Kosasih Padmawinata. Bandun: Penerbit ITB, 1988
7. Harborne JB. Phytochemical Methods (Indonesian: Metode Fitokimia Penuntun Cara Modern Menganalisis Tumbuhan). Penterjemah: B. Widianto. Bandung: Penerbit ITB. 1987
8. Mustarichie R, Ramdhani D, and Iskandar Y., "Characteristics and alopecia activity of pakis gajah (Angiopteris evecta (G.forst) Hoffman.) growing in galunggung mountainside, West Java," Asian Journal of Pharmaceutical and Clinical Research 2017; 10(11):337–340.
9. Mustarichie R, Sulistiyaningsih S, and Runadi D. Antibacterial Activity Test of Extracts and Fractions of Cassava Leaves (Manihot esculenta Crantz) against Clinical Isolates of Staphylococcus epidermidis and Propionibacterium acnes Causing Acne, International Journal of Microbiology Volume 2020, Article ID 1975904, 9 pageshttps://doi.org/10.1155/2020/1975904
10. Rahman N, Dewi NU, and Bohari B. Phytochemical and Antioxidant Activity of Avocado Leaf Extract (Persea americana Mill.). Asian Journal of Scientific Research, 2018; 11: 357-63.
11. Henry LN, Mtaita UYand Kimaro CC. Nutritional efficacy of avocado seeds, Glob. J. Food Sci. Technol. 2015; 193-6
12. Aletan U. Proximate and Physicochemical Analysis of the Fruit and Oil ofAvocado Pear, Communication in Physical Sciences 2018, 3(1): 18-26
13. Tcheghebe T, Nyamen LD, Tatongn NF, Seukep AJ. Ethnobotanical uses, phytochemical and pharmacological profile , and toxicity off Persea americana: an overview. Pharmacology online 2016; 3:213-21.
14. Shruti SR, Padma T. A Review on Persea americana Mill (Avocado) – Its Fruit and oil. Nt J Pharm Tech Res 2015; 8(6):72-77.
15. Falodun A, Erharuyi O, Imieje V., et al., In vitro evaluation of aliphatic fatty alcohol metabolites of Persea americana seed as potential antimalarial and antimicrobial agents. Nig Biotech 2014; 27:1-7.
16. Komlaga G, Cojean S, Mehdi Beniddir A. The antimalarial potential of three Ghanaian medicinal plants. Herbal Medicine 2015; 1(11) :1-6.
17. Adeyemi OO, Okpo SO, Ogunti Osencpre O. Analgesic and anti-inflammatory effects of the aqueous extract of leaves of Persea americana Mill (Lauraceae). Fitoterapi 2002; 73: 375-80.
18. Brai BI, Odetola AA, Agomo PU. Bartholomew I. Hypoglycemic and hypocholesterolemic potential of Persea americana leaf extracts. J Med Food 2007; 10:356-60.
19. Nayak BS, Raju SS, Chalapathi Rao AV. Wound healing activity of Persea americana (Avocado) fruit: a preclinical study on rats. J Wound Care 2008; 17:123-5
20. Marrero-Faz E, Sánchez-Calero J, Young L, et al., Inhibitory effect of Persea Americana Mill. Leaf aqueous extract and its fractions on PTP1B as therapeutic target for type 2 diabetes. Boletín Latino americano y del Caribe de Plantas Medicinales y Aromáticas 2014; 13(2): 144-51.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
AUTHORS WHO PUBLISH WITH THIS JOURNAL AGREE TO THE FOLLOWING TERMS:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).