Hepatoprotective Activity of Citrus Limetta on Wistar Albino Rats Hrishabh Jangid*, Mohammad Mukim, Rahul Ancheria, Ankit Sharma Kota College of Pharmacy, Kota, Rajasthan, India

  • Hrishabh Jangid Kota College of Pharmacy, Kota, Rajasthan, India
  • Mohammad Mukim Kota College of Pharmacy, Kota, Rajasthan, India
  • Rahul Ancheria Kota College of Pharmacy, Kota, Rajasthan, India
  • Ankit Sharma Kota College of Pharmacy, Kota, Rajasthan, India

Abstract

The methanolic extract of citrus limetta (MECL) peel was screened for hepatoprotective activity in hepatotoxic wistar albino rats induced via paracetamol (PCM) and carbon tetra chloride (CCL4). Hepatoprotective activity of MECL was measured by estimating biochemical parameters such as AST (aspartate transaminase), ALT (alanine aminotransferase), SGPT (serum glutamine pyruvate transferase), SGOT (serum glutamic oxaloacetic transaminase). It was observed that administration of PCM, CCL4 decrease the level of proteins and increased the level of serum marker enzymes which is an evidence of existence of hepatotoxicity in rats. The present results provide strong evidence that MECL inhibits hepatotoxicity induced by CCL4 and PCM. The hepatoprotective action was much more significant at the dose of 2ml/kg when compared to 1mg/kg.


 

Keywords: Citrus Limetta, Hepatoprotective Activity, Hepatotoxicity, Paracetamol, Carbon Tetra Chloride

Downloads

Download data is not yet available.

Author Biographies

Hrishabh Jangid, Kota College of Pharmacy, Kota, Rajasthan, India

Kota College of Pharmacy, Kota, Rajasthan, India

Mohammad Mukim, Kota College of Pharmacy, Kota, Rajasthan, India

Kota College of Pharmacy, Kota, Rajasthan, India

Rahul Ancheria, Kota College of Pharmacy, Kota, Rajasthan, India

Kota College of Pharmacy, Kota, Rajasthan, India

Ankit Sharma, Kota College of Pharmacy, Kota, Rajasthan, India

Kota College of Pharmacy, Kota, Rajasthan, India

References

1. A. H. Arbab, M. K. Parvez, M. S. Al-Dosari et al., “Hepatoprotective and antiviral efficacy of Acacia mellifera leaves fractions against hepatitis B virus,” BioMed Research International, vol. 2015, Article ID 929131, 10 pages, 2015.
2. Amresh, G., Rao, Ch.V., Parasnath Singh, 2007a, Antinociceptive and antiarthritic activity of Cissampelos pareira Roots. J. Ethnopharmacology, 111:531-536.
3. Anonymous 1966, Indian Pharmacopoeia, 2nd Edition, Government of India, New Delhi.
4. Anonymous 1999, Formulary of Siddha medicine. The Indian Medical practitioners’ com-operative Pharmacy and Stores, Ltd., Chennai.
5. B. A. Ahmed, “Hepatoprotective, antioxidant and antibacterial effects of Ocimum basilicum, Anogeissus leiocarpus, Khaya senegalensis and Capparis decidua against CCl4 induced hepatotoxicity in rats,” Sudan Academy of Sciences (SAS), Council of Bioscience, Advanced Technology and Environment, Sudan, 2015, PhD. thesis.
6. Bhatnagar, A.K., Popli, S.P., 1967.Chemical examination of the roots of Cissampelos pareira Linn. V. Structure and stereochemistry of hayatidin. Cellular and Molecular life Science 23, 242-243.
7. Bhattacharya, S., Saha, P., Gupta, M.,2011. Exploration of anti-inflammatory potential of Citrus limetta risso and citrus maxima, Pharmacology online 1, 702-709.
8. Bocco, S. S., Sanshy. H. Desa, A.M.H., Manar, A.C., Bovill, S. Rao., T.N.S.,1998. Antioxidant activity of phenolic composition of citrus peel and seed extract, Food chemistry 46, 2123-2129.
9. Buckley NA, Srinivasan J 2002. "Should a lower treatment line be used when treating paracetamol poisoning in patients with chronic alcoholism? a case for". Drug safety: an international journal of medical toxicology and drug experience 25 (9): 619–24. PMID 12137556.
10. C.-H. Lee, S.-W. Park, Y. S. Kim et al., “Protective mechanism of glycyrrhizin on acute liver injury induced by carbon tetrachloride in mice,” Biological & Pharmaceutical Bulletin, vol. 30, no. 10, pp. 1898–1904, 2007.
11. Cameron, A., Nappam, L.,2010. The pathogenesis of liver injury in carbon tetrachloride and thioacetamide. pp.322-331.
12. Candasmy, M., Srikakulam, V., 2010.Protective effect of livactine against CCl4 & PCM induced hepatotoxicity adult wistar rats. North America journal of medical sciences. Vol -2 49l-495.
13. Crippen JS, April 1993. "Acetaminophen hepatotoxicity: potentiation by isoniazid". The American journal of gastroenterology 88 (4): 590–2. PMID 8470644.
14. D. Scholten, J. Trebicka, C. Liedtke, and R. Weiskirchen, “The carbon tetrachloride model in mice,” Laboratory Animals, vol. 49, no. 1_suppl, pp. 4–11, 2015.
15. Dai Y, Cederbaum AI June 1995. "Cytotoxicity of acetaminophen in human cytochrome P4502E1-transfected HepG2 cells". The Journal of pharmacology and experimental therapeutics 273 (3): 1497–505.PMID 7791125.
16. Dargan PI, Jones AL 2002. "Should a lower treatment line be used when treating paracetamol poisoning in patients with chronic alcoholism? a case against". Drug safety: an international journal of medical toxicology and drug experience 25 (9): 625–32. PMID 12137557.
17. Dart.F. Keffrod., Harry.,2011. Chemical constituent of citrus fruit, vol-9,.285-287.
18. Gulati, R.K., Agarwal, S., Agarwal, S.S., 1995. Hepatoprotective studies on Phyllanthus emblica Linn. And quercetin. Indian Journal of Experimental Biology 33,261-268.
19. Guyton A.C., Hall, J.E.,1995. “Text Book of Medical physiology” 3rd edition, Elsevier, New Delhi, pp-134-152.
20. Handa, S.S., Sharma, A., 1990. Hepatoprotective activity of andrographolide from Andrographis paniculata against CCl4. Indian Journal of Medical Research 92, 276-283.
21. Handa, S.S., Sharma, A., Chakraborty, K.K.,1989. Natural products and plants as liver protecting drugs. Fitoterapia 57,307.
22. Heard KJ July 2008. "Acetylcysteine for acetaminophen poisoning". The New England journal of medicine 359 (3): 285–92. doi:10.1056/NEJMct0708278. PMID 18635433.
23. Huang, K.C., 1993.The pharmacology of Chinese Herbs. CRC Press, Boka Raton,Fl.pp388.
24. Irfan Alikhan., Atiya Khanum., 2005. Herbal medicines for Diseases, Ukaaz publications India. I,pp 289-290.
25. Jacob,Annie., Sumathy,V.,2010.Effect of citrus fruit peel extracts on pathogens causing gastrointestinal disorde. Vol-10,414-422.
26. Khan, S., Muhammad,K.,.2014. A comprehensive review on flavones., Journal of
27. Khushwaha Akhilesh., Gupta Vikas., 2012. Antimicrobial properties of peels of citrus fruits, International journal of universal pharmacy & life sciences.,pp. 2249-6793.
28. Limdi, J.K., Hyde, G.M., 2003. Evaluation of abnormal liver function tests. Postgraduate medical journal 79,pp.307-312.
29. M. A. Mohamed, I. A. A. Mohamed, T. H. Tag El-Din, A. Hassan, and M. Hassan, “Hepatoprotective effect of Adansonia digitata L. (Baobab) fruits pulp extract on CCl4-induced hepatotoxicity in rats,” World Journal of Pharmaceutical Research, vol. 4, no. 8, pp. 368–377, 2015.
30. M. R. Ahsan, K. M. Islam, I. J. Bulbul, M. A. Musaddik, and E. Haque, “Hepatoprotective activity of methanol extract of some medicinal plants against carbon tetrachloride-induced hepatotoxicity in rats,” European Journal of Scientific Research, vol. 37, no. 2, pp. 302–310, 2009.
31. M. Z. Zaruwa, N. I. Ibok, I. U. Ibok et al., “Effects of Sterculia setigera Del. Stem bark extract on haematological and biochemical parameters of wistar rats,” Biochemistry Insights, vol. 9, Article ID BCI.S36143, 2016.
32. Marina D.F, 1995. Multivariate analysis of flavones glycoside in citrus juices. Journal of Food 44,pp.255-264.
33. Marina Nu’nez., 2006. Hepatotoxicity of antiretrovirals:Incidence, mechanism and management. Journal of Hepatology 44,S132-139.
34. Mitchell JR, Jollow DJ, Potter WZ, Gillette JR, Brodie BB (October 1973). "Acetaminophen-induced hepatic necrosis. IV. Protective role of glutathione". The Journal of pharmacology and experimental therapeutics 187 (1): 211–7. PMID 4746329.
35. Monajemi, M., Polson J;2005. Cytotoxic effects of essential oil of some citrus peel. pp-183-187.
36. Muzna, S., Kamat,D.,2014. International Journal of Pharmacy and Pharmaceutical Sciences,vol- 6,53-56.
37. Neha Trivedi, Rawal,U.M.,2000.Hepatoprotective and toxicological evaluation of Andrographis paniculata on severe liver damage. Indian Journal of pharmacology 32. pp.288-293.
38. Nolan CM, Sandblom RE, Thummel KE, Slattery JT, Nelson SD 1994. "Hepatotoxicity associated with acetaminophen usage in patients receiving multiple drug therapy for tuberculosis". Chest 105 (2): 408–11. doi:10.1378/chest.105.2.408. PMID 7508362.
39. Owais,T.D., Ahamed,I., Khan, S., Khan,U., Ahamed,N., 2006. An alternate holistic medicinal approach to the total management of hepatic disorder: A novel polyherbal formulation. Modern phytomedicine. Turning medicinal plants in to drugs. Wiley-VCH Verlag GmbH Co. KGa. pp.223-245.
40. Pallab,K., Gupta, Malaya.,Bhattacharya,Sanjib.,2011.Evaluation of antihyperglycemic activity of citrus limetta fruit peel in streptozotocin –induced diabetic rats., International scholarity research network . ,150-154.
41. Perez,Y.Y., Alonso, D.,2010. Citrus limetta leaved antagonizes the hypertensive effect of angiotensin II. Ethnopharmacol5,611-614.
42. Schmidt LE, Dalhoff K, Poulsen HE (April 2002). "Acute versus chronic alcohol consumption in acetaminophen-induced hepatotoxicity". Hepatology 35 (4): 876–82. doi:10.1053/jhep.2002.32148.PMID 11915034.
43. Scott Luper,N.D.,1998. A review of plants used in the treatment of liver diseases: Alternative Medicine Review 3,410-419.
44. Sharma, M.P., Ahuja,V.,1997. Amoebic liver abscess: Clinician perspective. Bombay hospital Journal 39, 615-619.
45. Smuckler, E., 1996. Structural and functional changes in acute liver injury. Environmental Health Prospectives 15,13-25.
46. Subramonium, A., Pushpangadan,P.,1999. Development of Phytomedicines for liver diseases. Indian Journal of Pharmacology 31,166-175.
47. Tenenbein M 2004. "Acetaminophen: the 150 mg/kg myth". Journal of toxicology. Clinical toxicology 42 2: 145–8. doi:10.1081/CLT-120030939. PMID 15214618.
48. Ulicna, O.,Greaksac.M., Voncava,O., 2003. Hepatoprotective effect of rooibos tea on CCl4 induced liver damage in rats, Physiological research 52,461-466.
49. Umamaheswari, M., Mutiah, P.L ., 2012. In-vitro antioxidant activites of leaves, fruits and peel extracts of citrus. International journal of Phytopharmcy, vol-2,13-20.
50. Vuppalanchi R, Liangpunsakul S, Chalasani N March 2007. "Etiology of new-onset jaundice: how often is it caused by idiosyncratic drug-induced liver injury in the United States?" Am.J.Gastroenterol.102 3:558–62;quiz693.
51. Webner.R., Friis-Moller, N., Sabin,C., 2005. Liver related deaths among HIV-infected person. Europian AIDS Clinical society 10th European AIDS Conference.
52. Yoon Y-H., Yi H-Y, Hilton, M.E., 2005. Liver cirrhosis mortality in the United states.1970-2002. National institute on Alcohol abuse and alcoholism (NIAAA).
53. Zezulka A, Wright N September 1982. "Severe metabolic acidosis early in paracetamol poisoning". British medical journal (Clinical research ed.) 285 6345: 851–2
Statistics
86 Views | 106 Downloads
How to Cite
Jangid, H., Mukim, M., Ancheria, R., & Sharma, A. (2021). Hepatoprotective Activity of Citrus Limetta on Wistar Albino Rats Hrishabh Jangid*, Mohammad Mukim, Rahul Ancheria, Ankit Sharma Kota College of Pharmacy, Kota, Rajasthan, India. Asian Journal of Pharmaceutical Research and Development, 9(1), 110-116. https://doi.org/https://doi.org/10.22270/ajprd.v9i1.890