Identification of gyrA Gene Resistance on Bacteria Escherichia coli and Klebsiellapneumoniaein Urinary Tract Infection Patients in Haji Adam Malik Center Hospital, Medan, Indonesia
Objectives: To find out whether Escherichia coli and Klebsiellapneumoniae that cause urinary tract infection have ciprofloxacin resistant gyrA coding genes.
Interventions: The method used in this study is a cross sectional prospective design and carried out prospectively in urinary tract infection patients who have medical record data on the diagnosis urinary tract infection patients in the period February 2019 to January 2020 at the Haji Adam Malik General Hospital in Medan by takingurine from patients, then detected using Polymerase Chain Reaction (PCR) in the Integrated Laboratory of the Faculty of Medicine, Universitas Sumatera Utara.
Main outcomes measure: The resistance coding gene in Escherichia coliwith the percentage of gyrA gene is 96,1% and Klebsiellapneumoniae has a low percentage where the expression of gyrA gene is 66,6%.
Conclusion: The distribution of the gyrA gene in Escherichia coli is higher than that of Klebsiellapneumoniae.
2. Lindayanti., Muzahar., dan Abdurrahim Rasyid Lubis. Pola Resistensi Antimikroba pada Ifeksi Saluran Kemih yang di sebabkan Oleh Bakteri Penghasil ESBL dan Non ESBL. The Journal Medical School, University of Sumatera Utara. Medan. 2014; (47):17-20.
3. Lobel B, Valot A, Cattoir V. Lemenand O, Gaillot O, Comparison of antimicrobial susceptibility of 1.217 Escherichia coli isolates from women with hospital and community-acquired urinary tract I nfections. Presse Med 2008; (37):746-50 [in French].
4. Sugianli, A.K., Franciscus, G., Lia K., Emmy, H.P., Ayodhia, P.P., Firza, G., Suzanne, G., Ida, P., Menno, D.D.J., Frank, V.L and Constance, S.Antimicrobial Resistance in Uropathogens and Appropriateness of Empirical Treatment: A Population-based Surveillance Study in Indonesia. Journal of Antimicrobial Chemotherapy. 2017; (72):1469-1477.
5. Hooper DC, Jacoby GA. Topoisomerase inhibitors: fluoroquinolone mechanisms of action and resistance. Cold Spring Harb Perspect Med 2016; (6):a025320.
6. Redgrave LS, Sutton SB, Webber MA, Piddock LJ. Fluoroquinolone resistance: mechanisms, impact on bacteria, and role in evolutionary success. Trends Microbiol 2014; (22):438-445.
7. Mirzaii, Mehdi., Sanaz. Jamshidi., Maryam Zamanzadeh. Determination of gyrA and parC mutations and prevalence of plasmid mediated quinolone resistence genes in Escherichia coli and Klebsiella pneumoniae isolated from patients with urinary tract infection in Iran. Journal of Global Antimicrobial Resistance.2018; (13):187-200.
8. Rachmad, Basuki., Wiria Saputri., Yandri, A.S., Andi Setiawan., Mulyono. Isolaai dan Identifikasi Gen Resisten Ciprofloxacin dari Penderita ISK di RSUDAM Provinsi Lampung. Jurnal Kedokteran. 2017; (3):487-497.
9. Notoatmodjo, S. (2011).Kesehatan Masyarakat Ilmudan Seni. Jakarta: Penerbit Rineka Cipta. p 45.
10. Lisdawati, V., I Parwati, Sudarmon, TM., Sudiro, R., R. Ramadhany., N.Puspandari., et al.Studi Pemetaan Awal DNA Mycobacterium komplekstuber kulosis Padaumumnya Spoligomengetik Pada Hasil Isolasi Dahak PasienTuberkulosis Parudari Kota Propinsi. Buletin Penelitian Kesehatan 2010; 38 (4):169-185.
11. Rubstova MY, Ulyashova MM, Bachmann TT, Schmid RD, Egorov AM. Multiparametric Determination Of Genes And Their Point Mutations For Identification Of Betalactamase. Biochemistry. 2010; 75(13): 1628-49.
12. Hout B. et al. Drug resistance in bacteria Isolated from Patients Presenting with Wounds At A Nonprofit Surgical Center in Phnom Penh, Cambodia from 2011–2013. Tropical Diseases Travel Medicine and Vaccines. 2015; 1(4).
13. Murakami K., W Minamide, K Wada, E Nakamura, H Teraoka, S Watanabe. Identification of Methicillin-Reistant Strains of Staphylococci by Poymerase Chain Reaction. Journal of Clinical Microbiology. 1991; 2(10):2240-2244.
14. Moon DC, Seol SY, Gurung M, Jin JS, Choi CH et al. Emergence of a new mutation and its accumulation in the topoisomerase IV gene confers high levels of resistance to fluoroquinolones in Escherichia coli isolates. Int J Antimicrob Agents 2010; 35:76-79.
15. Correia S, Hebraud M, Chafsey I, Chambon C, VialaD et al. Impacts of experimentally induced and clinically acquired quinolone resistance on the membrane and intracellular subproteomes of Salmonella Typhimurium DT104B. J Proteomics 2016; 145: 46-59.
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