Inhibitory AKT2 And Vegr2 Genes Expression of Avicennia Marina Using RT-PCR On Widr Cells
DOI:
https://doi.org/10.22270/ajprd.v8i1.641Keywords:
Akt2, VEGFR2,WiDr cells, Avicennia marina, RT-PCR.Abstract
Objectives: To evaluate the activities the anticancer of n-hexane extract of Avicennia marina leaves on WiDr cells in down-regulated of expression of Akt2 and VEGFR2 genes.
Methods: Avicennia marina leaves were dried and extracted with n-hexane, analyzed the down-regulated Akt 2 and VEGFR2 expression which was determined the Reverse Transcription Polymerase Chain Reaction (RT-PCR) method.
Results:N-hexane extract of Avicennia marina, in which there were down regulated expression Akt 2 and VEGFR2 of 0.43 and 0.50 WiDr cells. N-hexane extract of mangrove leaves has cancer chemoprevention activity with down-regulated on WiDr cells.
Conclusions: :N-hexane extract of Avicennia marina leaves had anticancer activity with down-regulated on WiDr cells, suggest that significantly inhibit the expression of Akt2 and VEGFR2 genes.
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