n-Hexane Extract of Mangrove Leaves Effect on p21 and Akt 2 Gene Expressions of WiDr Cells

Authors

  • Istiqomah M A Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia
  • Hasibuan P. A. Z Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia
  • Basyuni M Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Medan, 20155, Indonesia

DOI:

https://doi.org/10.22270/ajprd.v7i6.624

Keywords:

Rhizophora mucronata, Nypa fruticans, leaves, Ceriops tagal, daun

Abstract

Objectives: The study aims to examine the anticancer effect of polyisoprenoid of Nypa fruticans, Ceriops tagal, and Rhizophora mucronata leaves in WiDr cells by evaluating the regulation of p21 and Akt 2 gene.

Design: Nypa fruticans, Ceriops tagal, and Rhizophora mucronata leaves were dried and extracted with n-hexane, analyzed the increase or decrease in regulation of p21 gene and Akt 2 expression which was determined the Reverse Transcription Polymerase Chain Reaction (RT-PCR) method.

Interventions: The variable that was intervened in this study was the 3 sample mangrove leaves.

Main outcome measure: The main measurement results in this study were to study n-hexane extracts of mangrove leaves able to suppress the expression of p21 and Akt 2 genes so that cancer cell growth is inhibited.

Results: n-hexane extract of Ceriops tagal leaves was more effective than Nypa fruticans and Rhizophora mucronata, in which there was up-regulated (p21) of 1.19 and down regulated (Akt 2) of 0.78 on colon cancer cells (WiDr). N-hexane extract of mangrove leaves has cancer chemoprevention activity with up-regulated and down-regulated on WiDr cells, in which the sample was more effective than n-hexane extract of Ceriops tagal leaves.

Conclusion: N-hexane extract of mangrove leaves had cancer chemoprevention activity with up-regulated and down-regulated on WiDr cells, in which the sample was more effective than n-hexane extract of Ceriops tagal leaves.

 

 

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Author Biographies

Istiqomah M A, Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia

Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia

Hasibuan P. A. Z, Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia

Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia

Basyuni M, Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Medan, 20155, Indonesia

Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Medan, 20155, Indonesia

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Published

2019-12-15

How to Cite

A, I. M., Z, H. P. A., & M, B. (2019). n-Hexane Extract of Mangrove Leaves Effect on p21 and Akt 2 Gene Expressions of WiDr Cells. Asian Journal of Pharmaceutical Research and Development, 7(6), 20–24. https://doi.org/10.22270/ajprd.v7i6.624