PHYTOCEMICALS SCREENING AND CELL CYCLE ARREST ACTIVITY OF n-HEXANE EXTRACT OF Vernonia amygdalina Delile LEAVES AGAINST PANCREATIC CANCER CELL LINE
DOI:
https://doi.org/10.22270/ajprd.v7i4.533Keywords:
Vernonia amygdalina Delile, Phytochemcial, Cytotoxic, Cell Cycle, apoptosisAbstract
Objective: This study aims to determined phytochemical compounds of simplex and n-hexane extract (nHE) of Vernonia amygdalina Delile Leaves and cell cycle arrest activity against PANC-1 cells.
Methods: The leaves of Vernonia amygdalina Delile were dried and extracted with n-hexane, followed by evaporation and freeze-drying. Phytochemicals screening were analyzed with standard procedures. Cytotoxic activity was carried out using MTT assay method. PANC-1 cells were treated with different concentrations of nHE (500 ug/mL, 250 ug/mL, 125 ug/mL, 61.5 ug/mL and 31.25 ug/mL) for 24 hours to obtained IC50 values. The cell cycle inhibition activity of nHE was carried out used flowcytometry method and apoptosis activity used double staining method.
Results: Then HE was identified contains steroids/triterpenoids. The IC50 was114.80 ± 1.21 ug/mL. The nHE inhibited cell cycle PANC-1 on M1 phase (67.39%) and it was induced apoptosis process on PANC-1 cells.
Conclusions: Our results suggest that extractsn-hexane of Vernonia amygdalina Delile Leaves had as cancer chemoprevention activities with inhibits cell cycle and spur apoptosis process on PANC-1 cells.
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