Spectrophotometric determination of ranitidine hydrochloride (RNH) in pharmaceutical formulation using 9-fluorenylmethyl chloroformate (FMOC-Cl)

  • Abdalla Ahmed Elbashir University of Khartoum, Faculty of science, Department of Chemistry, Khartoum , Sudan
  • Shahd Moutasim Merghani University of Khartoum, Faculty of science, Department of Chemistry, Khartoum 11115, Sudan

Abstract

A new, simple and sensitive spectrophotometric method is developed for the determination of ranitidine hydrochloride (RNH). The proposed method is based upon reaction of RNH with 9-fluorenylmethyl chloroformate (FMOC-Cl) in borate buffer of pH 8.0 producing an absorption maximum at 255 nm. All parameters required for the reaction conditions are investigated. Linearity is verified with a range of 2-16 μg/mL and is described by the regression equation y = 61129 x + 0.0354 with a correlation coefficient of 0.9998 (n = 7). The limit of detection (LOD) and the limit of quantification (LOQ) were calculated as per ICH guidelines and were found to be 0.2219 and 0.6724 μg/mL, respectively. The method was successfully applied for the determination of RNH in pharmaceutical formulation. Therefore, the method can be used for routine analysis of RNH in quality control laboratories.

Keywords: Spectrophotometric, Ranitidine hydrochloride (RNH), 9-Fluorenylmethyl chloromate (FMOC-Cl).

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Author Biographies

Abdalla Ahmed Elbashir, University of Khartoum, Faculty of science, Department of Chemistry, Khartoum , Sudan

Prof. Abdalla Ahmed Elbashir, University of Khartoum, Faculty of Science, Department of Chemistry, Khartoum 11115, Sudan,

Shahd Moutasim Merghani, University of Khartoum, Faculty of science, Department of Chemistry, Khartoum 11115, Sudan

University of Khartoum, Faculty of science, Department of Chemistry, Khartoum 11115, Sudan

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How to Cite
Elbashir, A., & Merghani, S. (2018). Spectrophotometric determination of ranitidine hydrochloride (RNH) in pharmaceutical formulation using 9-fluorenylmethyl chloroformate (FMOC-Cl). Asian Journal of Pharmaceutical Research and Development, 6(6), 7-14. https://doi.org/https://doi.org/10.22270/ajprd.v6i6.454