TERPENOIDS AND PHYTOSTEROLS ISOLATED FROM PLUMERIA RUBRA L., FORM ACUTIFOLIA (AIT) WOODSON AND ITS FUNGUS PARASITE COLESPORIUM PLUMERIAE

  • Resende, C. M Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), University City, Rio de Janeiro. Brazil
  • Barreto A. S Laboratory of Chemical and Biological Analysis (LAQB), Foundation State University Center of the West Zone (UEZO), Avenue Manuel Caldeira de Alvarenga, Campo Grande, Rio de Janeiro, Brazi
  • Pinto, P. R. Laboratory of Chemical and Biological Analysis (LAQB), Foundation State University Center of the West Zone (UEZO), Avenue Manuel Caldeira de Alvarenga, Campo Grande, Rio de Janeiro, Brazil
  • Nascimento C. C. H. C Laboratory of Chemical and Biological Analysis (LAQB), Foundation State University Center of the West Zone (UEZO), Avenue Manuel Caldeira de Alvarenga, Campo Grande, Rio de Janeiro, Brazil.
  • Vasconcelos, S. D. D. De Federal Center of Technological Education Celso Suckow da Fonseca- CEFET-NI, Rio de Janeiro, RJ- Brazil
  • Azevedo, L. A.C. Laboratory of Chemical and Biological Analysis (LAQB), Foundation State University Center of the West Zone (UEZO), Avenue Manuel Caldeira de Alvarenga, Campo Grande, Rio de Janeiro, Brazi
  • Stephens P. R. S. 3Laboratory of Innovations in Therapies, Teaching and Bioproducts/LITEB, OswaldoCruz Institute, IOC/ FIOCRUZ), Rio de Janeiro, Brazil.
  • Nogueira R. I. Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Rio de Janeiro, Brazil.
  • Saranraj, P. Department of Microbiology, Sacred Heart College (Autonomous), Tirupattur, Tami Nadu, India.
  • Diré G. F. Laboratory of Chemical and Biological Analysis (LAQB), Foundation State University Center of the West Zone (UEZO), Avenue Manuel Caldeira de Alvarenga, Campo Grande, Rio de Janeiro, Brazi
  • Pinto, A. C Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), University City, Rio de Janeiro. Brazil.

Abstract

The hexane and ethyl acetate extracts of the leaves from Plumeria rubra L., form acutifolia (Ait) Woodson and the methanolic extract from the fungus Coleosporium plumeriae Pat. were analized by high resolution gas chromatography (HRGC) and by high resolution gas chromatography coupled to mass spectrometes (GC-MS). The identification of the chemical constituintes is these extracts has been made by the automatic comparison of the mass spectra obtained standards within the NBS, Wiley and Wiley 275T librarys supplied with the mass spectrometer and by analized of the principal fragmentations. Phytochemical studies of the hexane and ethyl acetate extracts of the leaves showed nine terpenoids (5-ergosten-3b-ol, 5,22-stigmastadien-3b-ol, 4-stigmasten-3-one, 5-stigmasten-3b-ol, 12-ursen-3b-ol, 12-oleanen-3b-ol, 12-oleanen-3-one-28-oic acid, ursolic acid and 3b-acetoxy-12-oleanen-28-oic acid). The compounds 5-ergosten-3b-ol, 4-stigmasten-3-one, 12-oleanen-3-one-28-oic acid, ursolic acid and 3b-acetoxy-12-oleanen-28-oic acid have been described for the first time from Plumeria rubra L., forma acutifolia (Ait) Woodson. From the methanolic extract of spores of the fungus Coleosporium plumeriae Pat were isolated 5-stigmasten-3b-ol, methyl esters of long chain fatty acids (C18:0, principally) and one aromatic compound, N-methyl toluenesulfonamide. No previous phytochemical studies of this fungus have been reported.

Keywords: Plumeria rubra L., form acutifolia, Coleosporium plumeriae Pat, terpenoids, phytosterols GC/ MS, HRGC.

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References

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How to Cite
M, R., S, B., R., P., H. C, N., De, V., A.C., A., R. S., S., I., N., P., S., F., D., & C, P. (2018). TERPENOIDS AND PHYTOSTEROLS ISOLATED FROM PLUMERIA RUBRA L., FORM ACUTIFOLIA (AIT) WOODSON AND ITS FUNGUS PARASITE COLESPORIUM PLUMERIAE. Asian Journal of Pharmaceutical Research and Development, 6(6), 15-22. https://doi.org/https://doi.org/10.22270/ajprd.v6i6.428