Stability Indicating RP-HPLC Method for Selegiline Hydrochloride in Pharmaceutical Formulation

Authors

  • M. A. Nagras Sinhgad College of Pharmacy, Department of Quality Assurance Techniques, Vadgaon (Bk.), Pune – 411041, India
  • Rahul C. Kalaskar Sinhgad College of Pharmacy, Department of Quality Assurance Techniques, Vadgaon (Bk.), Pune – 411041, India

Keywords:

Selegiline Hydrochloride, RP-HPLC, Validation and Degradation

Abstract

Stability indicating RP-HPLC method has been developed for the determination of Selegiline hydrochloride in pharmaceutical formulation. Chromatographic separation of Selegiline hydrochloride and its known impurities was achieved by using BDS Hypersil C18 (250 x 4.6 mm, 5 µm) column at temperature of 40˚C. Mobile phase composed ofAcetonitrile:Potassium di-hydrogen phosphate buffer adjusted at pH 4.0+0.5 (30:70v/v). Flow rate was 1.00 ml per min and the absorbance was measured at 205nm. The retention time of Selegiline hydrochloride was 5.7min, at runtime 10min. System suitability parameters like, tailing factor 1.1 and theoretical plates 6010 were calculated. The linearity of the method was evaluated from 25µg per ml to 100µg per ml for Selegiline hydrochloride by injecting 50µl of standard solution giving correlation coefficient 0.999. The developed method can be used or applied for the quantification of Selegiline hydrochloride in pharmaceutical products.

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Published

2014-07-01

How to Cite

Nagras, M. A., & Kalaskar, R. C. (2014). Stability Indicating RP-HPLC Method for Selegiline Hydrochloride in Pharmaceutical Formulation. Asian Journal of Pharmaceutical Research and Development, 2(4), 5–13. Retrieved from https://ajprd.com/index.php/journal/article/view/204

Issue

Volume 2nd Issue 4th July-Agust 2014

Section

Research Articles

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