Gap Analysis and Recent Advances in HPLC and UPLC
DOI:
https://doi.org/10.22270/ajprd.v14i5.1878Abstract
Objective: To systematically review the advances of High-Performance Liquid Chromatography (HPLC) and Ultra-Performance Liquid Chromatography (UPLC) since the year of 2020 in the fields of analytical performance, sustainability, automation, and emerging technologies.
Data Sources: Databases were searched for all literature related to HPLC and UPLC published from 2020 to 2026 using Scopus, PubMed, Web of Science, and Google Scholar as resources.
Study Selection: A total of fifty-five peer-reviewed papers were chosen based upon predefined eligibility criteria to be included as part of this literature surveythese comprised fifteen reviews, twenty-nine articles, and eleven abstracts.
Summary of Contents: Advances in the following areas are reviewed: Sub-2 µm and superficially porous columns, Q-TOF MS, green analytical chemistry metrics, automated processes, AI, and miniaturized sample preparation. Challenges include limited transfers of existing methods, lack of open access databases, and inadequate validation of AI-assisted method development by regulatory bodies.
Conclusion: HPLC and UPLC have the potential to evolve into faster, more eco-friendly, and innovative analytical platforms. Addressing current limitations will allow for further integration of HPLC and UPLC with nanotechnology, microfluidics, and sustainable analytical chemistry.
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