Formulation and Evaluation of Poly-herbal Hair Oil

Abstract

Hair and scalp disorders caused by bacterial pathogens such as Staphylococcus epidermidis, Staphylococcus aureus, and Propionibacterium acnes are prevalent dermatological concerns that significantly impair hair health, aesthetics, and patient quality of life. Synthetic antimicrobial agents, while effective, are associated with increasing microbial resistance, scalp irritation, and long-term toxicity concerns. There is therefore a compelling need for naturally derived, safe, and efficacious alternatives.

This study presents the formulation and comprehensive evaluation of an antibacterial hair oil prepared from a combination of six botanically active ingredients: curry leaves (Murrayakoenigii), hibiscus (Hibiscus rosa-sinensis), black cumin seeds (Nigella sativa), fenugreek seeds (Trigonellafoenum-graecum), aloe vera gel (Aloe barbadensis miller), and coconut oil (Cocos nucifera). The formulation was subjected to physicochemical characterization (pH, viscosity, specific gravity, refractive index, acid value, saponification value), organoleptic evaluation, phytochemical screening, antimicrobial activity testing via the agar disc-diffusion method, stability studies, and patch test for safety evaluation.

Results demonstrated that the formulated hair oil displayed significant physicochemical parameters were within acceptable ranges and the preparation showed good activity. No signs of skin irritation were observed in the patch test. These findings validate the therapeutic and cosmetic potential of the polyherbal hair oil formulation as a safe, effective, and sustainable alternative to synthetic products for scalp health management.