Formulation and Evaluation of Antimicrobial Activity of Marine Red Algae Gracilaria Corticata
DOI:
https://doi.org/10.22270/ajprd.v13i3.1555Abstract
Objective: The present study was carried out to investigate the antimicrobial activity of formulated gel containing marine red algae Gracilaria Corticata belonging to the family Rhodophyta by disc diffusion assay method against bacterial and fungal organisms.
Methods: The ethanolic extract of Gracilaria Corticatawere subjected to phytochemical and physiochemical analysis and then gel prepared using an ethanolic extract of Gracilariacorticata were divided into two different concentration such as 250 and 500 µg. The formulated gel was investigated for antimicrobial activity by disc diffusion assay method against S. aureus, P. aeruginosa, and Candida albicans and compared with standard drug ketoconazole (30µg) and amikacin (30µg). The zones of inhibition formed against organisms were calculated.
Results: In the present study the formulated gel showed a moderately potent antimicrobial activity. The zone of inhibition of prepared gel against S. Aureus and P. aeruginosa was found to be 12 and 10 mm respectively. The zone of inhibition of prepared candida albicans was found to be 12 mm. And the phytochemical analysis shows the presence of active constituents such as flavonoids, sugar, phenol and quinones and physiochemical analysis shows that the antimicrobial activity is due to the presence of active constituents and evaluation of gel proves the stability of the formulation.
Conclusion: The present study shows that the invitro anti-microbial activity of the formulated gel at the dose of 500 was higher inhibitory against virulent bacteria which cause various infection. From the above studies, it confirms the formulated gel exhibit moderately potent antimicrobial activity.
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References
Nivedita Sahu , Dina Bandhu Sahoo , study of morphology and agar contents in some important gracilaria species of Indian coast, American journal of plant sciences, 2013;4;52-59.
Pesarameli karteek, vellanki jahnavi, D.V keerthi, k. Chaitanya saravathi, Evaluation of antibacterial activity of herbs, the international research journal of pharmacy, 2012;3(8):230-232.
Kenneth C. Ofokansi1, Anthony A. Attama1, Philip F. Uzor, and Mercy O. Ovri, Evaluation of the combined antimicrobial activity of the leaf extract of Phyllanthus muellerians with ciprofloxacin. journal of pharmaceutical technology and drug research, 2013;2:16, 2-6
Mrinalini J Singh1, C. K. Raadha, Studies on the Antimicrobial Potency of the Marine Algae - Gracilaria corticata var cylindrical and Hydroclathrus clathratus, journal of advance in life science and health, February 2015;2:1(50-55).
S. Sangeetha, N.B. dhayanithi and N.sivakumar ,antibacterial activity of sargassam longifolium and gracilaraiacorticata from gulf of mannar against selected common shrimpspathogens , international journal of pharma and biosciences, 2014; 5(2):76-82
Abhilash parameswaran kailas and muraleedharan Sukumaran nair , saponins and the invitro bioactivities of different solvent extracts of some topical green and red seaweeds ,journal of coastal life medicine 2013; 3(12):931-943
K. Kolanjinathan and D.stella , Pharmacological effect of gracilaria corticata solvent extracts against human pathogenic bacteria and fungi , international journal of pharmaceutical and biological archives 2011; 2(6):1722-1728.
Prasad M.P, shekhar Sushant, rindhe ganesh , antibacterial activity of seaweed gracilaria species extract against infectious pathogens , Asian journal of biological and life science ,sep 2012;1: 219-222
Mahsin j jamadar, rajmahammad husen shaikh, preparation and evaluation of herbal gel formulation, journal of pharmaceutical research and education, 2017; 1(2):201-224
Ajinkya M. banker, menjusha n. dole, formulation and evaluation of herbal antimicrobial gel containing musa acuminata leaves extract, journal of pharmacognosy and phytochemistry ,2016; 5(1): 01-03
Sudipta das, pallab k haldar, goutam pramanik . formulation and evaluation of herbal gel containing clerodendron infortunatum leaves extract, international journal of pharma tech research 2011; 3(1):140-143.
Rajasekaran aiyalu, arulkumaran govindarajan , arivukkarasu Ramasamy , formulation and evaluation of topical herbal gel for the treatment of arthritis in animal model , Brazilian journal of pharmaceutical sciences, 2016; l52(3):(493-507)
K. Kolanjinathan and D.stella , Pharmacological effect of gracilaria corticata solvent extracts against human pathogenic bacteria and fungi , international journal of pharmaceutical and biological archives 2011;2(6):1722-1728.
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