Ocimum Sanctum: Phytochemistry, Therapeutic Uses Pharmacological Activities and Its Anticancer Activities

Authors

  • Santosh Kumar S.R. 1Assistant professor, Department of Studies in Food Technology, Davangere University, Shivagangothri, Davangere-577007, India.
  • Manoj Manjunath Bongale Research Scholar Department of Studies in Biotechnology, ShivaGangotri Campus Davangere University, Karnataka, India.
  • Prakash Pralhad Sarwade Associate Professor and Head, Department of Botany, Shikshan Maharshi Guruvarya R. G. Shinde Mahavidyalaya, Paranda Dist. Dharashiv (Osmanabad) 413502, (M.S.) India.
  • K.Vijayalakshmi Research scholar, PG dept of Zoology, V.O. Chidambaram college, Thoothukudi- 8, Affiliated by Manonmanium sundaranar University, Thirunelveli, Tamil Nadu,  India. 
  • Mamta Goswami Associate Professor, Faculty of Pharmaceutical Sciences, Amrapali University, Lamachaur Haldwani, India
  • Rapborlang Khongshei Public Relation Officer, KG Hospital, No. 5, Govt Arts College Road, Coimbatore-641018 Tamil Nadu, India.
  • Kavita Narayan Gaisamudre(Sarwade) Assistant Professor, Department of Botany, Shriman Bhausaheb Zadbuke Mahavidyalaya, Barshi Tal. Barshi, Dist- Solapur 413 401 Maharashtra, India

DOI:

https://doi.org/10.22270/ajprd.v13i2.1239

Abstract

The practice of Ayurveda, which is based on the principles of living a healthy lifestyle and eating adaptogenic herbs on a regular basis, has the potential to alleviate a significant number of the chronic diseases that are the main cause of mortality across the globe. Tulasi, also known as Ocimum sanctum Linn, is considered to be the most significant herb in Ayurvedic medicine. Recent scientific research has demonstrated that it imparts a number of positive health effects. An increasing body of research indicates that the unique combination of pharmacological properties that tulsi possesses has the potential to alleviate stress on various levels, including the physical, physiological, metabolic, and psychological levels. Tulsi has been found to protect internal organs and tissues from the damaging effects of heavy metals and industrial pollutants, as well as against the impacts of physical stresses such as cold, excessive loudness, ischemia, and prolonged durations of physical exercise. This has been demonstrated through research. Plants that have medicinal properties are utilized on a daily basis by traditional healers in order to treat a wide variety of ailments. Ocimum sanctum Linn, a small herb that is widely distributed across India and is referred to as Tulsi in Hindi, has a long history of application in traditional medicine for the treatment of a wide range of ailments. These ailments include bronchitis, bronchial asthma, malaria, diarrhea, dysentery, skin diseases, arthritis, painful eye diseases, chronic fever, insect bites, and many others. There is some evidence to suggest that O. sanctum L. may possess qualities that include antibacterial, anticancer, antidiabetic, neuroprotective, and hepatoprotective. It has been demonstrated that O. sanctum L. possesses therapeutic value, as demonstrated by the pharmacological research that was incorporated into this study.

 

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Author Biographies

Santosh Kumar S.R., 1Assistant professor, Department of Studies in Food Technology, Davangere University, Shivagangothri, Davangere-577007, India.

Assistant professor, Department of Studies in Food Technology, Davangere University, Shivagangothri, Davangere-577007, India.

Manoj Manjunath Bongale, Research Scholar Department of Studies in Biotechnology, ShivaGangotri Campus Davangere University, Karnataka, India.

Research Scholar Department of Studies in Biotechnology, ShivaGangotri Campus Davangere University, Karnataka, India.

Prakash Pralhad Sarwade, Associate Professor and Head, Department of Botany, Shikshan Maharshi Guruvarya R. G. Shinde Mahavidyalaya, Paranda Dist. Dharashiv (Osmanabad) 413502, (M.S.) India.

Associate Professor and Head, Department of Botany, Shikshan Maharshi Guruvarya R. G. Shinde Mahavidyalaya, Paranda Dist. Dharashiv (Osmanabad) 413502, (M.S.) India.

K.Vijayalakshmi, Research scholar, PG dept of Zoology, V.O. Chidambaram college, Thoothukudi- 8, Affiliated by Manonmanium sundaranar University, Thirunelveli, Tamil Nadu,  India. 

Research scholar, PG dept of Zoology, V.O. Chidambaram college, Thoothukudi- 8, Affiliated by Manonmanium sundaranar University, Thirunelveli, Tamil Nadu,  India. 

Mamta Goswami, Associate Professor, Faculty of Pharmaceutical Sciences, Amrapali University, Lamachaur Haldwani, India

Associate Professor, Faculty of Pharmaceutical Sciences, Amrapali University, Lamachaur Haldwani, India

Rapborlang Khongshei, Public Relation Officer, KG Hospital, No. 5, Govt Arts College Road, Coimbatore-641018 Tamil Nadu, India.

Public Relation Officer, KG Hospital, No. 5, Govt Arts College Road, Coimbatore-641018 Tamil Nadu, India.

Kavita Narayan Gaisamudre(Sarwade), Assistant Professor, Department of Botany, Shriman Bhausaheb Zadbuke Mahavidyalaya, Barshi Tal. Barshi, Dist- Solapur 413 401 Maharashtra, India

Assistant Professor, Department of Botany, Shriman Bhausaheb Zadbuke Mahavidyalaya, Barshi Tal. Barshi, Dist- Solapur 413 401 Maharashtra, India

References

Siva M, Shanmugam KR, Shanmugam B, Venkata SG, Ravi S, Sathyavelu RK, Mallikarjuna K. Ocimum sanctum: a review on the pharmacological properties. Int. J. Basic Clin. Pharmacol. 2016 May;5(3):558-65.

Pandey G, Madhuri S. Pharmacological activities of Ocimum sanctum (tulsi): a review. Int J Pharm Sci Rev Res. 2010 Nov;5(1):61-6.

Singh D, Chaudhuri PK. A review on phytochemical and pharmacological properties of Holy basil (Ocimum sanctum L.). Industrial Crops and Products. 2018 Aug 1;118:367-82.

Mahajan N, Rawal S, Verma M, Poddar M, Alok S. A phytopharmacological overview on Ocimum species with special emphasis on Ocimum sanctum. Biomedicine & Preventive Nutrition. 2013 Apr 1;3(2):185-92.

Rahman S, Islam R, Kamruzzaman M, Alam K, Jamal AH. Ocimum sanctum L.: A review of phytochemical and pharmacological profile. American journal of drug discovery and Development. 2011;1:1-5.

Pattanayak P, Behera P, Das D, Panda SK. Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview. Pharmacognosy reviews. 2010 Jan;4(7):95.

Kumar R, Saha P, Lokare P, Datta K, Selvakumar P, Chourasia A. A systemic review of Ocimum sanctum (Tulsi): Morphological characteristics, phytoconstituents and therapeutic applications. International Journal for Research in Applied Sciences and Biotechnology. 2022 Apr 7;9(2):221-6.

Prakash PA, Gupta N. Therapeutic uses of Ocimum sanctum Linn (Tulsi) with a note on eugenol and its pharmacological actions: a short review. Indian journal of physiology and pharmacology. 2005 Apr 1;49(2):125.

Singh D, Chaudhuri PK. A review on phytochemical and pharmacological properties of Holy basil (Ocimum sanctum L.). Industrial Crops and Products. 2018 Aug 1;118:367-82.

Rahman S, Islam R, Kamruzzaman M, Alam K, Jamal AH. Ocimum sanctum L.: A review of phytochemical and pharmacological profile. American journal of drug discovery and Development. 2011;1:1-5.

Baseer M, Jain K. Review of Botany, Phytochemistry, Pharmacology, Contemporary applications and Toxicology of Ocimum sanctum. International Journal of Pharmacy & Life Sciences. 2016 Feb 1;7(2).

Mandal AK, Poudel M, Neupane NP, Verma A. Phytochemistry, pharmacology, and applications of Ocimum sanctum (Tulsi). InEdible Plants in Health and Diseases: Volume II: Phytochemical and Pharmacological Properties 2022 Mar 15 (pp. 135-174). Singapore: Springer Singapore.

Pradhan D, Biswasroy P, Haldar J, Cheruvanachari P, Dubey D, Rai VK, Kar B, Kar DM, Rath G, Ghosh G. A comprehensive review on phytochemistry, molecular pharmacology, clinical and translational outfit of Ocimum sanctum L. South African Journal of Botany. 2022 Nov 1;150:342-60.

Zahran EM, Abdelmohsen UR, Khalil HE, Desoukey SY, Fouad MA, Kamel MS. Diversity, phytochemical and medicinal potential of the genus Ocimum L.(Lamiaceae). Phytochemistry Reviews. 2020 Aug;19:907-53.

Chaudhary A, Sharma S, Mittal A, Gupta S, Dua A. Phytochemical and antioxidant profiling of Ocimum sanctum. Journal of Food Science and technology. 2020 Oct;57(10):3852-63.

Hanumanthaiah P, Panari H, Chebte A, Haile A, Belachew G. Tulsi (Ocimum sanctum)–a myriad medicinal plant, secrets behind the innumerable benefits. Arabian Journal of Medicinal and Aromatic Plants. 2020 Apr 14;6(1):105-27.

Enegide C. Ocimum Species: ethnomedicinal uses, phytochemistry and pharmacological importance. International Journal of Current Research in Physiology and Pharmacology. 2021 May 24:1-2.

Prakash PA, Gupta N. Therapeutic uses of Ocimum sanctum Linn (Tulsi) with a note on eugenol and its pharmacological actions: a short review. Indian journal of physiology and pharmacology. 2005 Apr 1;49(2):125.

Pattanayak P, Behera P, Das D, Panda SK. Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview. Pharmacognosy reviews. 2010 Jan;4(7):95.

Singh N, Verma P, Pandey BR, Bhalla M. Therapeutic potential of Ocimum sanctum in prevention and treatment of cancer and exposure to radiation: An overview. International Journal of pharmaceutical sciences and drug research. 2012 Jul 4;4(2):97-104.

Singh V, Amdekar S, Verma O. Ocimum sanctum (tulsi): Bio-pharmacological activities. Webmed Central Pharmacol. 2010 Oct 22;1(10):1-7.

Siva M, Shanmugam KR, Shanmugam B, Venkata SG, Ravi S, Sathyavelu RK, Mallikarjuna K. Ocimum sanctum: a review on the pharmacological properties. Int. J. Basic Clin. Pharmacol. 2016 May;5(3):558-65.

Chaudhary A, Sharma S, Mittal A, Gupta S, Dua A. Phytochemical and antioxidant profiling of Ocimum sanctum. Journal of Food Science and technology. 2020 Oct;57(10):3852-63.

Rithichai P, Jirakiattikul Y, Nambuddee R, Itharat A. Effect of Salicylic Acid Foliar Application on Bioactive Compounds and Antioxidant Activity in Holy Basil (Ocimum sanctum L.). International Journal of Agronomy. 2024;2024(1):8159886.

Ashokkumar M, Palanisamy K, Ganesh Kumar A, Muthusamy C, Senthil Kumar KJ. Green synthesis of silver and copper nanoparticles and their composites using Ocimum sanctum leaf extract displayed enhanced antibacterial, antioxidant and anticancer potentials. Artificial Cells, Nanomedicine, and Biotechnology. 2024 Dec 31;52(1):438-48.

Pathak I, Niraula M, Sharma KR, Thapa P, Oli HB, Kalauni SK. Green Synthesis of Silver Nanoparticles from Ocimum sanctum Linn. and Study of Their Antioxidant Activity. Amrit Research Journal. 2024 Dec 31;5(1):82-90.

Zahid H, Saeed F, Lateef M. Anti-urease and antioxidant activity on selected medicinal plants: Nigella sativa, Ricinus communis, Ocimum sanctum and Curcuma longa. Hamdard Medicus. 2024;67(1).

Kumar A, Agarwal K, Maurya AK, Shanker K, Bushra U, Tandon S, Bawankule DU. Pharmacological and phytochemical evaluation of Ocimum sanctum root extracts for its antiinflammatory, analgesic and antipyretic activities. Pharmacognosy magazine. 2015 May;11(Suppl 1):S217.

Varghese RM, Kumar A, Shanmugam R. Comparative anti-inflammatory activity of silver and zinc oxide nanoparticles synthesized using Ocimum tenuiflorum and Ocimum gratissimum herbal formulations. Cureus. 2024 Jan;16(1).

Bhattarai K, Bhattarai R, Pandey RD, Paudel B, Bhattarai HD. A Comprehensive Review of the Phytochemical Constituents and Bioactivities of Ocimum tenuiflorum. The Scientific World Journal. 2024;2024(1):8895039.

Dhanapal P, Shanmugam R, MD PM. Ocimum gratissimum mediated synthesis of AgNPs–An in vitro analysis of anti-inflammatory and antimicrobial effects. Med J Malaysia. 2025 Jan 1;80:1.

Sharma AD, Kaur I, Angish S, Thakur A, Sania S, Singh A. Comparative phytochemistry, antioxidant, antidiabetic, and anti-inflammatory activities of traditionally used Ocimum basilicum L. Ocimum gratissimum L., and Ocimum tenuiflorum L. BioTechnologia. 2022;103(2):131.

Mallikarjun S, Rao A, Rajesh G, Shenoy R, Pai M. Antimicrobial efficacy of Tulsi leaf (Ocimum sanctum) extract on periodontal pathogens: An: in vitro: study. Journal of Indian Society of Periodontology. 2016 Mar 1;20(2):145-50.

Al Ahdal K, Maawadh AM, Al Deeb L, Alshamrani AS, Almohareb T, Alrahlah A. Effect of malachite green, ocimum sanctum, and Er, Cr: YSGG laser on antimicrobial activity against S. mutans and CAD disinfection bonded to resin restoration. Photodiagnosis and Photodynamic Therapy. 2023 Jun 1;42:103571.

Panigrahi S, Rath CC. In vitro characterization of antimicrobial activity of an endophytic bacterium Enterobacter cloacae (MG001451) isolated from Ocimum sanctum. South African Journal of Botany. 2021 Dec 1;143:90-6.

Rai A, Singh A, Sharma A. Phytochemical analysis & antimicrobial activity of the leaves of Ocimum sanctum. Journal of Pharmacognosy and Phytochemistry. 2022;11(3):174-6.

Nasim I, Jabin Z, Kumar SR, Vishnupriya V. Green synthesis of calcium hydroxide-coated silver nanoparticles using Andrographis paniculata and Ocimum sanctum Linn. leaf extracts: An antimicrobial and cytotoxic activity. Journal of Conservative Dentistry and Endodontics. 2022 Jul 1;25(4):369-74.

Chaiyana W, Punyoyai C, Sriyab S, Prommaban A, Sirilun S, Maitip J, Chantawannakul P, Neimkhum W, Anuchapreeda S. Anti‐Inflammatory and Antimicrobial Activities of Fermented Ocimum sanctum Linn. Extracts against Skin and Scalp Microorganisms. Chemistry & Biodiversity. 2022 Feb;19(2):e202100799.

Mousavi L, Salleh RM, Murugaiyah V. Antidiabetic and In Vitro Enzyme Inhibition Studies of Methanol Extract of Ocimum tenuiflorum Linn Leaves and Its Fractions. Tropical life sciences research. 2020 Apr;31(1):141.

Kesevan RK, Norakma MN, Zaibunnisa AH, Astuti PD. Total phenolic content and anti-diabetic activity of dried and fresh leaves of Ocimum sanctum (Linn). Food Research. 2023;7(4):139-45.

Vijay A, Nivethitha L, Mooventhan A. Anti-diabetic effect and it’s mechanism of action of Ocimum species (basil leaves) in type 2 diabetes mellitus: A narrative review. Advances in Integrative Medicine. 2024 Aug 14.

Arya R, Faruquee HM, Shakya H, Rahman SA, Begum MM, Biswas SK, Apu MA, Islam MA, Sheikh MM, Kim JJ. Harnessing the Antibacterial, Anti-Diabetic and Anti-Carcinogenic Properties of Ocimum sanctum Linn (Tulsi). Plants. 2024 Dec 16;13(24):3516.

Islam MT. Anti-diabetic potential of Ocimum sanctum Linn. Int J Adv Chem Res. 2022;6:38-41.

Mishra S, Sundaram S, Srivastava S, Dhar R. Phytosynthesis of Silver Nanoparticles Using Oscimum sanctum Leaf Extract and Studies on Its Antidiabetic, Antioxidant, and Antibacterial Properties. ACS Applied Bio Materials. 2023 Sep 26;6(10):4127-37.

Lahon K, Das S. Hepatoprotective activity of Ocimum sanctum alcoholic leaf extract against paracetamol-induced liver damage in Albino rats. Pharmacognosy research. 2011 Jan;3(1):13.

Kamel FO, Karim S, Bafail DA, Aldawsari HM, Kotta S, Ilyas UK. Hepatoprotective effects of bioactive compounds from traditional herb Tulsi (Ocimum sanctum Linn) against galactosamine-induced hepatotoxicity in rats. Frontiers in pharmacology. 2023 Oct 4;14:1213052.

Chatterjee A, Sarkar B. Polyphenols and terpenoids derived from Ocimum species as prospective hepatoprotective drug leads: a comprehensive mechanistic review. Phytochemistry Reviews. 2024 Jul 12:1-43.

Teofilović B, Tomas A, Martić N, Stilinović N, Popović M, Čapo I, Grujić N, Ilinčić B, Rašković A. Antioxidant and hepatoprotective potential of sweet basil (Ocimum basilicum L.) extract in acetaminophen-induced hepatotoxicity in rats. Journal of Functional Foods. 2021 Dec 1;87:104783.

Madhavilatha J, Kothamasu S, Kattepogu NK. Phytochemical profile, anti-oxidant and hepatoprotective activity Ocimum tenuiflorum leaves against carbon tetrachloride–induced hepatotoxicity in Mice. International Journal of Current Innovations in Advanced Research. 2024 Apr 1:15-35.

Almatroodi SA, Alsahli MA, Almatroudi A, Rahmani AH. Ocimum sanctum: role in diseases management through modulating various biological activity. Pharmacognosy Journal. 2020;12(5).

Venuprasad MP, Hemanth Kumar K, Khanum F. Neuroprotective effects of hydroalcoholic extract of Ocimum sanctum against H 2 O 2 induced neuronal cell damage in SH-SY5Y cells via its antioxidative defence mechanism. Neurochemical research. 2013 Oct;38:2190-200.

Mataram MB, Hening P, Harjanti FN, Karnati S, Wasityastuti W, Nugrahaningsih DA, Kusindarta DL, Wihadmadyatami H. The neuroprotective effect of ethanolic extract Ocimum sanctum Linn. in the regulation of neuronal density in hippocampus areas as a central autobiography memory on the rat model of Alzheimer’s disease. Journal of chemical neuroanatomy. 2021 Jan 1;111:101885.

Nandini HS, Krishna KL, Apattira C. Combination of Ocimum sanctum extract and Levetiracetam ameliorates cognitive dysfunction and hippocampal architecture in rat model of Alzheimer’s disease. Journal of Chemical Neuroanatomy. 2022 Mar 1;120:102069.

Sarangi SC, Pattnaik SS, Katyal J, Kaleekal T, Dinda AK. An interaction study of Ocimum sanctum L. and levetiracetam in pentylenetetrazole kindling model of epilepsy. Journal of ethnopharmacology. 2020 Mar 1;249:112389.

Rodrigues V, Rao MS, Rao GS, KG MR. Neuroprotective potential of ocimum sanctum (Linn) leaf extract in preventing and attenuating stress induced substantia nigral neuronal damage in rats. Journal of Ayurveda and Integrative Medicine. 2022 Oct 1;13(4):100651.

Pattnaik SS, Sarangi SC, Sharma D, Sinha S, Nag TC, Nambirajan A, Tripathi M. Ocimum sanctum extract preserves neuronal echotexture and controls seizure in lithium-pilocarpine induced status epilepticus rats. Asian Pacific Journal of Tropical Biomedicine. 2023 Nov 1;13(11):479-87.

Seyed MA, Ayesha S, Azmi N, Al-Rabae FM, Al-Alawy AI, Al-Zahrani OR, Hawsawi Y. The neuroprotective attribution of Ocimum basilicum: A review on the prevention and management of neurodegenerative disorders. Future Journal of Pharmaceutical Sciences. 2021 Dec;7:1-4.

Harsha M, Kumar KM, Kagathur S, Amberkar VS. Effect of Ocimum sanctum extract on leukemic cell lines: A preliminary: in-vitro: study. Journal of Oral and Maxillofacial Pathology. 2020 Jan 1;24(1):93-8.

Hasan MR, Alotaibi BS, Althafar ZM, Mujamammi AH, Jameela J. An update on the therapeutic anticancer potential of Ocimum sanctum L.:“Elixir of life”. Molecules. 2023 Jan 25;28(3):1193.

Perna S, Alawadhi H, Riva A, Allegrini P, Petrangolini G, Gasparri C, Alalwan TA, Rondanelli M. In vitro and in vivo anticancer activity of basil (Ocimum spp.): Current insights and future prospects. Cancers. 2022 May 11;14(10):2375.

Luke AM, Patnaik R, Kuriadom ST, Jaber M, Mathew S. An in vitro study of Ocimum sanctum as a chemotherapeutic agent on oral cancer cell-line. Saudi Journal of Biological Sciences. 2021 Jan 1;28(1):887-90.

Ahmad N, Ansari MA, Al-Mahmeed A, Joji RM, Saeed NK, Shahid M. Biogenic silver nanomaterials synthesized from Ocimum sanctum leaf extract exhibiting robust antimicrobial and anticancer activities: Exploring the therapeutic potential. Heliyon. 2024 Aug 15;10(15).

Boonyanugomol W, Rukseree K, Prapatpong P, Reamtong O, Baik SC, Jung M, Shin MK, Kang HL, Lee WK. An in vitro anti-cancer activity of Ocimum tenuiflorum essential oil by inducing apoptosis in human gastric cancer cell line. Medicina. 2021 Jul 30;57(8):784.

Khatoon S, Kalam N, Balasubramaniam VR, Shaikh MF, Ansari MT. Chemotherapeutic role of polyphenols present in Ocimum sanctum. Anti-Cancer Agents in Medicinal Chemistry-Anti-Cancer Agents). 2022 Dec 1;22(20):3325-42.

Manaharan T, Thirugnanasampandan R, Jayakumar R, Kanthimathi MS, Ramya G, Ramnath MG. Purified essential oil from Ocimum sanctum Linn. triggers the apoptotic mechanism in human breast cancer cells. Pharmacognosy magazine. 2016 May;12(Suppl 3):S327.

Published

2025-04-15

How to Cite

Santosh Kumar S.R., Manoj Manjunath Bongale, Prakash Pralhad Sarwade, K.Vijayalakshmi, Mamta Goswami, Rapborlang Khongshei, & Kavita Narayan Gaisamudre(Sarwade). (2025). Ocimum Sanctum: Phytochemistry, Therapeutic Uses Pharmacological Activities and Its Anticancer Activities. Asian Journal of Pharmaceutical Research and Development, 13(2), 119–125. https://doi.org/10.22270/ajprd.v13i2.1239

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