RP-HPLC Method Development for Simultaneous Estimation of Azilsartan Medoxomil and Chlorthalidone In Pharmaceutical Formulations

Authors

  • Amol Kakde Faculty of Pharmaceutical Sciences, Ram Krishna Dharmarth Foundation University, Bhopal, M.P.
  • Neha Jain Faculty of Pharmaceutical Sciences, Ram Krishna Dharmarth Foundation University, Bhopal, M.P.

Abstract

Simple, accurate, economical and reproducible RP-HPLC method for simultaneous estimation of two component drug mixture AzilsartanMedoxomil and Chlorthalidone in combined tablet dosage form has been developed. Developed HPLC method is reversed phase chromatographic method using Inertsil C18 column and gradient mobile phase, detection of eluent was carried out using PDA detector at a flow rate of 1.2ml/ min. Forced degradation studies under various stress conditions, including hydrolysis, oxidation, thermal, humidity, and photolytic degradation, were performed. The developed method was validated in terms of specificity, linearity, precision, intermediate precision, accuracy, robustness and solution stability. The linearity was observed in concentration range of 12.5 ppm to 37.5 ppm for AzilsartanMedoxomil and from 3.5 ppm to 22.7ppm for Chlorthalidone. Results of analysis were validated statistically and by recovery studies. The proposed RP-HPLC method achieved satisfactory resolution between AzilsartanMedoxomil and Chlorthalidone. It can be used for determination of AzilsartanMedoxomil in drug substance and pharmaceutical preparation. Thismethod is suitable for routine quality control and stability testing of AzilsartanMedoxomil and Chlorthalidone in pharmaceutical formulations.

 

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Author Biographies

Amol Kakde, Faculty of Pharmaceutical Sciences, Ram Krishna Dharmarth Foundation University, Bhopal, M.P.

Faculty of Pharmaceutical Sciences, Ram Krishna Dharmarth Foundation University, Bhopal, M.P.

Neha Jain, Faculty of Pharmaceutical Sciences, Ram Krishna Dharmarth Foundation University, Bhopal, M.P.

Faculty of Pharmaceutical Sciences, Ram Krishna Dharmarth Foundation University, Bhopal, M.P.

References

AAAAAAAAAAAAAAAAAA

Published

2025-02-15

How to Cite

Amol Kakde, & Neha Jain. (2025). RP-HPLC Method Development for Simultaneous Estimation of Azilsartan Medoxomil and Chlorthalidone In Pharmaceutical Formulations. Asian Journal of Pharmaceutical Research and Development, 13(2), 164–169. Retrieved from https://ajprd.com/index.php/journal/article/view/1201

Issue

Vol. 13 No. 2 (2025): Volume 13 Issue 2 March- April. 2025

Section

Research Articles

Copyright (c) 2025 Amol Kakde, Neha Jain

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