Polymerase Chain Reaction Analysis of Pork DNA in Unauthorized Jamu and Fish Oil Capsule Shells

  • Ridho Asra School of Pharmaceutical Science (STIFARM), Padang, Indonesia
  • Fitra Fauziah School of Pharmaceutical Science (STIFARM), Padang, Indonesia
  • Gemmy Sarina School of Pharmaceutical Science (STIFARM), Padang, Indonesia
  • Rahmi Zhavira Hasan School of Pharmaceutical Science (STIFARM), Padang, Indonesia
  • Ghina Muharrani Yahya School of Pharmaceutical Science (STIFARM), Padang, Indonesia

Abstract

Capsules for pharmaceutical dosage forms are generally manufactured from beef gelatin. However, several reports claimed the alleged misuse of pork gelatin was improperly used in producing illegitimate capsules for the Muslim community. This study aimed to analyze pork DNA in unregistered jamu and fish oil capsules using the Polymerase Chain Reaction (PCR) method. The sample was isolated to obtain pure pork DNA and amplified using a specific primer to generate millions of DNA using the multi-step PCR method. The PCR product was analyzed to identify the pork DNA's existence via gel electrophoresis in 1.5% agarose. The analysis showed that several samples were positive at 132 bp compared to reference pork DNA. The result of this study revealed that unregistered products should be evaluated and followed up on to prevent product abuse.


 

Keywords: Capsule, DNA, PCR, Pork

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Author Biographies

Ridho Asra, School of Pharmaceutical Science (STIFARM), Padang, Indonesia

School of Pharmaceutical Science (STIFARM), Padang, Indonesia

Fitra Fauziah, School of Pharmaceutical Science (STIFARM), Padang, Indonesia

School of Pharmaceutical Science (STIFARM), Padang, Indonesia

Gemmy Sarina, School of Pharmaceutical Science (STIFARM), Padang, Indonesia

School of Pharmaceutical Science (STIFARM), Padang, Indonesia

Rahmi Zhavira Hasan, School of Pharmaceutical Science (STIFARM), Padang, Indonesia

School of Pharmaceutical Science (STIFARM), Padang, Indonesia

Ghina Muharrani Yahya, School of Pharmaceutical Science (STIFARM), Padang, Indonesia

School of Pharmaceutical Science (STIFARM), Padang, Indonesia

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How to Cite
Asra, R., Fauziah, F., Sarina, G., Hasan, R., & Yahya, G. (2022). Polymerase Chain Reaction Analysis of Pork DNA in Unauthorized Jamu and Fish Oil Capsule Shells. Asian Journal of Pharmaceutical Research and Development, 10(5), 19-22. https://doi.org/https://doi.org/10.22270/ajprd.v10i5.1175