Acute Toxicity of Ethanolic Extract of Leucaena leucocephala (Lam) de Wit Seeds in Wistar Rats

  • Irzal Fanany Department of Pharmacy Biology, Faculty of Pharmacy, University of Sumatera Utrara, Medan, Indonesia
  • Marline Nainggolan Department of Pharmacy Biology, Faculty of Pharmacy, University of Sumatera Utrara, Medan, Indonesia
  • Yuandani Y Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia
  • Saddam Husein Department of Pharmacy Biology, Faculty of Pharmacy, University of Sumatera Utrara, Medan, Indonesia

Abstract

Objective: This study was conducted to investigate the toxic effect of ethanol extract of Leucaena leucocephala(Lam) de Wit seeds(EELLS) in rats.


Method: simplicia powder of L.leucochepala seeds extracted by maceration with 80% of ethanol. Evaluation of toxicity effect used the fixed dose method which refer to OECD (Organization of Economic Cooperation and Development)420.


Result: Acute toxicity evaluation in this experiment using different doses varying from 50-2000mg/kgbw, did not result in the death of any animals until 14 days of observation in the experimental evaluation period and there was no change in behavior towards rats.


Conclusion: These results have shown that oral administration of L. leuceocephala did not produce any significant toxic effect in rats. Hence, L. leucocephala could be regarded as a safe natural product for therapeutic use


 

Keywords: L.leucocephala, acute toxicity, ethanolic extract

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Author Biographies

Irzal Fanany, Department of Pharmacy Biology, Faculty of Pharmacy, University of Sumatera Utrara, Medan, Indonesia

Department of Pharmacy Biology, Faculty of Pharmacy, University of Sumatera Utrara, Medan, Indonesia

Marline Nainggolan, Department of Pharmacy Biology, Faculty of Pharmacy, University of Sumatera Utrara, Medan, Indonesia

Department of Pharmacy Biology, Faculty of Pharmacy, University of Sumatera Utrara, Medan, Indonesia

Yuandani Y, Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia

Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia

Saddam Husein, Department of Pharmacy Biology, Faculty of Pharmacy, University of Sumatera Utrara, Medan, Indonesia

Department of Pharmacy Biology, Faculty of Pharmacy, University of Sumatera Utrara, Medan, Indonesia

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How to Cite
Fanany, I., Nainggolan, M., Y, Y., & Husein, S. (2021). Acute Toxicity of Ethanolic Extract of Leucaena leucocephala (Lam) de Wit Seeds in Wistar Rats. Asian Journal of Pharmaceutical Research and Development, 9(1), 43-45. https://doi.org/https://doi.org/10.22270/ajprd.v9i1.920