The Activity of Combination of Hydrolyzed Virgin Coconut Oil and Chitosan Toward Wound Healing Parameters on NIH 3T3 Cells Using in Vitro Methods
DOI:
https://doi.org/10.22270/ajprd.v7i3.534Keywords:
Rhizomucor miehei, viability, proliferation, migration, expressionAbstract
Objectives: The objective of this study was to investigate the activity of combination of hydrolyzed VCO (HVCO) and chitosan on NIH 3T3 cell proliferation activity, NIH 3T3 cell migration, COX-2 and VEGF protein expression.
Design: In vitro cytotoxic assay was determined by MTT (MicrocultureTetrazoliumTehnique) assay, cell proliferation activity was measured by calculating cell viability incubated 24 hours, 48 hours and 72 hours, wound closure percentage was tested by scratch wound healing method, expression of COX-2 protein and VEGF protein were measured by immunocytochemical method.
Interventions: The variable that was intervened in this study was the concentration of HVCO and chitosan.
Main Outcome Measures: The main measurements carried out in this study were the absorbance value of HVCO and chitosan which was converted into viability cell, proliferation activity, percentage of wound closure, and percentage of COX-2 and VEGF protein expression.
Results: Cytotoxic activity of HVCO and chitosan resulted the best concentration at 31.25 μg/ml, scratch wound healing assay from a combination HVCO and chitosan resulted the best migration of fibroblast cells at a ratio of 1:1 with HVCO 62.5 μg/ml and chitosan 62.5 μg/ml, combination of HVCO 62.5 μg/ml and chitosan 62.5 μg/ml (1:1) increased expression of COX-2 and VEGF.
Conclusion: Combination of HVCO and chitosan could increase NIH 3T3 cell migration, COX-2 and VEGF protein expression. Combination of HVCO and chitosan had better wound healing activity in vitro than single use.
Keywords: Rhizomucor miehei, viability, proliferation, migration, expression
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2. Wang PH, Huang BS, Horng HC, Yeh CC, Chen YJ. Wound Healing. Journal of the Chinese Medical Association. 2018; 81: 94-101.
3. Soliman AM, Lin TS, Ghafar NA, Das S. Virgin Coconut Oil and Diabetic Wound Healing: Histopathological and Biochemical Analysis. European Journal of Anatomy. 2018; 22(2):135-44.
4. Lieberman S, Enig MG, Preuss HG. A Review of Monolaurin and Lauric Acid Natural Virucidal and Bactericidal Agent. Alternative & Complementary Therapies. 2006; 12(6):310-314.
5. Silalahi J, Surbakti C. Burn Wound Healing Activity of Hydrolyzed Virgin Coconut Oil. International Journal of PharmaTech Research. 2015; 8(1):67-73.
6. Silalahi J, Permata YM, Putra ED. Antibacterial Activity of Hydrolyzed Virgin Coconut Oil. Asian Journal Pharmaceutical and Clinical Research. 2014; 7(2):90-4.
7. Silalahi J, Situmorang P, Patilaya P, Silalahi YC. Antibacterial Activity of Chitosan and Hydrolized Coconut Oil and Their Combination against Bacillus cereus and Escherichia coli. Asian Journal of Pharmaceutical and Clinical Research. 2016; 9(5): 69-73.
8. Loung FS, Silalahi J, Suryanto D. Antibacterial Activity of Enzymatic Hydrolized of Virgin Coconut Oil and Palm Kernel Oil Against Staphylococcus aureus, Salmonella thypii and Escherichia coli. International Journal of PharmaTech Research. 2014; 6:628-33.
9. Margata L, Silalahi J, Harahap U, Satria D. The Effect of Dietary Oils and Hydrolyzed Coconut Oil on Minerals Absorption in Rats. Asian Journal Pharmaceutical and Clinical Research. 2018; 11:185-90.
10. Cancer Chemoprevention Research Center. Fixed Procedure of Sample Preparation. Faculty of Pharmacy, University of Gadjah Mada; 2009. p. 1-3.
11. Satria D, Silalahi J, Haro G, Ilyas S, Hasibuan PAZ.Cell Cycle Inhibition of Ethylacetate Fraction of Zanthoxylum Acanthopodium DC. Fruit against T47D Cells. Macedonian Journal of Medical Sciences. 2019; 7(5):726—29.
12. Hasibuan PAZ, Sumaiyah. The Anti-Proliferative and Pro-Apoptotic Properties of Ethanol Plectranthusamboinicus (Lour.) Spreng. Leaves Ethanolic Extract Nanoparticles on T47D Cell Lines. Asian Pacific Journal of Cancer Prevention. 2019; 20(3):897-901.
13. Harahap U, Hasibuan PAZ, Sitorus P, Arfian N, Satria D. Antimigration Activity of an Ethylacetate Fraction of Zanthoxylum acanthopodium DC. Fruits in 4T1 Breast CancerCells. Asian Pacific Journal of Cancer Prevention. 2018; 19(2):565-69.
14. Nugroho AE, Hermawan A, Putri DDP, Novika A, Meiyanto E. Combinational Effects of Hexane Insoluble Fraction of Ficusseptica Burm. F. and doxorubicin chemotherapy on T47D breast cancer cells. Asian Pacific Journal of Tropical Biomedicine. 2013; 3(4):297-302.
15. Dayrit FM. The Properties of Lauric Acid and Their Significance in Coconut Oil. Journal of the American Oil Chemists' Society. 2015; 92:1-15.
16. Wild T, Rahbarnia A, Kellner M, Sobotka L. Basic in Nutrition and Wound Healing. Nutrition. 2015; 26:862-66.
17. Kim SK. Chitin, Chitosan, Oligosaccharides and Their Derivates: Biological Activities and Applications. CRC Press; 2011. p. 13, 461-73.
18. Cheung RCF, Ng TB, Wong JH, Chan WY. Chitosan: An Update On potential Biomedical and Pharmaceutical Aplications. Marine Drugs. 2015; 13:5156-86.
19. Nevin KG, Rajamohan T. Effect of Topical Application of Virgin Coconut Oil on Skin Components and Antioxidant Status during Dermal Wound Healing in Young Rats. Skin Pharmacology and Physiology. 2010; 23:290-97.
20. Miura S, Tatsuguchi A, Wada K, Takeyama H, Shinji Y, Hiratsuka T, Futagami S, Miyake K, Gudis K, Mizokami Y, Matsuoka T, Sakamoto C. Cyclooxygenase-2-Regulated Vascular Endothelial Growth Factor Release in Gastric Fibroblasts. American Journal of Physiology Gastrointestinal and Liver Physiology. 2004; 287:444-51.
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