Antimicrobial and Antioxidant Activity of Pomegranate Peel Extracts Obtained by Sequential Extraction Method

  • Missa Mohammed Saleh Abdealsiede Department of Chemistry, Central Laboratory, Ministry of Higher Education & Scientific Research, P. O. Box  Office 7099,  Khartoum, Sudan
  • Ayat Ahmed Alrasheid Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences and Technology,  Khartoum, Sudan
  • Mei Musa Ali Omar 2Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan
  • Abdalla Ahmed Elbashir Department of Chemistry, Faculty of Science, University of Khartoum, Khartoum, Sudan

Abstract

Punica granatum L. (Pomegranate) is a plant belongs to Lythraceae family. The objective of this study was to evaluate the antimicrobial activity of  petroleum ether, chloroform , acetone, ethyl acetate and ethanol extracts from the peel fruit of Punica granatum against standard microorganism.  This plant has been used as a traditional treatment for several diseases such as microbial infections. Extracts were evaluated for their effectiveness against four bacterial strains including both Gram-positive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria as well as fungal species (Candida albicans and Aspergillus niger) using disc diffusion method. The ethyl acetate, acetone and petroleum ether extracts showed higher antibacterial activity than the chloroform and ethanol extracts with inhibition zone ranged between (7-16 mm). The petroleum ether and chloroform extracts showed the higher antifungal activity compared with the other extracts, the diameter inhibition zone ranged between (11-16 mm). The antioxidant potential of peel extracts was determined on the basis of their scavenging activity of the stable 1, 1-diphenyl-2-picryl hydrazyl (DPPH) free radical and 2,2'azino-bis(ethyl benzthiazoline -6-sulfonic acid (ABTS+). All extracts showed high antioxidant activity . The highest result of antioxidant activity by DPPH scavenging assay was in acetone extract (89.40%), but high result by ABTS in ethanol extract (72.07%).


KEYWORDS: 

Keywords: Punicagranatum L. peel , antibacterial activity, antifungal, antioxidant activity, sequential extraction

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Author Biographies

Missa Mohammed Saleh Abdealsiede, Department of Chemistry, Central Laboratory, Ministry of Higher Education & Scientific Research, P. O. Box  Office 7099,  Khartoum, Sudan

Department of Chemistry, Central Laboratory, Ministry of Higher Education & Scientific Research, P. O. Box

 Office 7099,  Khartoum, Sudan

Ayat Ahmed Alrasheid, Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences and Technology,  Khartoum, Sudan

Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences and Technology,

 Khartoum, Sudan

Mei Musa Ali Omar, 2Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan

Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences and Technology,

 Khartoum, Sudan

Abdalla Ahmed Elbashir, Department of Chemistry, Faculty of Science, University of Khartoum, Khartoum, Sudan

Department of Chemistry, Faculty of Science, University of Khartoum, Khartoum, Sudan

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How to Cite
Abdealsiede, M. M., Alrasheid, A., Ali Omar, M., & Elbashir, A. (2020). Antimicrobial and Antioxidant Activity of Pomegranate Peel Extracts Obtained by Sequential Extraction Method. Asian Journal of Pharmaceutical Research and Development, 8(2), 14-20. https://doi.org/https://doi.org/10.22270/ajprd.v8i2.710