Wound Healing Activities of Hydrolyzed Virgin Coconut Oil (HVCO) and Fucoidan Combination: An In Vitro Assay

Authors

  • Evayanti Meiliana Sagala Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia
  • Jansen Silalahi Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.22270/ajprd.v7i3.532

Keywords:

wound healing, MTT, viability, proliferation, HVCO, fucoidan, migration, immunocytochemistry

Abstract

combination, in the NIH 3T3 cell line using in vitro assay, and compared with single HVCO and single fucoidan. Methods: NIH 3T3 Cell viability and proliferation were assessed using the MTT method, migration activity was assessed using scratch wound healing assays and expression of COX-2 and VEGF protein were determined using immunocytochemistry (ICC). Results: The results from the proliferative activity assay show that the effective concentrations for all samples were 31.25 μg /ml. NIH 3T3 cells migration activity assay showed that the best combination of the HVCO and fucoidan was 50:50. From COX 2 and VEGF protein expression test results, the combination of HVCO and fucoidan has a higher percentage of expression than single HVCO or single fucoidan Conclusion: The results reveal that the combination of HVCO and fucoidan has better wound healing activity than single HVCO or single fucoidan

 

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Author Biographies

Evayanti Meiliana Sagala, Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia

Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia

Jansen Silalahi, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia

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Published

2019-06-15

How to Cite

Sagala, E. M., & Silalahi, J. (2019). Wound Healing Activities of Hydrolyzed Virgin Coconut Oil (HVCO) and Fucoidan Combination: An In Vitro Assay. Asian Journal of Pharmaceutical Research and Development, 7(3), 40–45. https://doi.org/10.22270/ajprd.v7i3.532