Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Lansoprazole, Clarithromycin and Tinidazole in Combined Dosage Form

  • Bharat Dadhich Dept of Pharmaceutical Chemistry Mahatma Gandhi College of Pharmaceutical Sciences, Jaipur, Rajasthan, India
  • Rakesh Goyal Dept of Pharmaceutical Chemistry Mahatma Gandhi College of Pharmaceutical Sciences, Jaipur, Rajasthan, India
  • Dilip Agarwal Dept of Pharmaceutical Chemistry Mahatma Gandhi College of Pharmaceutical Sciences, Jaipur, Rajasthan, India

Abstract

Analytical chemistry is one of the supreme branches of science which deals with the separation, identification and quantification of various natural and chemical compounds. It includes both qualitative and quantitative analysis. Quantitative analysis is having importance from the point of pharmaceutical chemistry. Quantification methods include chromatographic techniques, spectrophotometery, fluorimetery etc. One of the most popular method for quantitative analysis is High Performance Liquid Chromatography (HPLC). In this article, a reverse phase – HPLC method was developed for the simultaneous determination of Lansoprazole, Clarithromycin and Tinidazole in combined dosage form and validation of the developed method. The development of method includes selection of mobile phase, chromatographic method and wavelength whereas validation involves the parameters like linearity, accuracy, precision, Limit of detection (LOD), Limit of Quantification (LOQ), robustness, system suitability of the developed method. The result showed that the developed method is best fitted to the simultaneous determination of Lansoprazole, Clarithromycin and Tinidazole and validated as per standards.


 

Keywords: Validation, HPLC, accuracy, precision

Downloads

Download data is not yet available.

Author Biographies

Bharat Dadhich, Dept of Pharmaceutical Chemistry Mahatma Gandhi College of Pharmaceutical Sciences, Jaipur, Rajasthan, India

Dept of Pharmaceutical Chemistry Mahatma Gandhi College of Pharmaceutical Sciences, Jaipur, Rajasthan, India

Rakesh Goyal, Dept of Pharmaceutical Chemistry Mahatma Gandhi College of Pharmaceutical Sciences, Jaipur, Rajasthan, India

Dept of Pharmaceutical Chemistry Mahatma Gandhi College of Pharmaceutical Sciences, Jaipur, Rajasthan, India

Dilip Agarwal, Dept of Pharmaceutical Chemistry Mahatma Gandhi College of Pharmaceutical Sciences, Jaipur, Rajasthan, India

Dept of Pharmaceutical Chemistry Mahatma Gandhi College of Pharmaceutical Sciences, Jaipur, Rajasthan, India

References

1. Douglas A Skoog, Donald M West, James F Holler, Stanley R Crouch. 2007. Fundamentals of Analytical Chemistry. 8th edn., Thomson Asia Pvt. Ltd: Singapore; 4, 921, 975.
2. Sharma BK. 2002. Instrumental Methods of Chemical Analysis. 22nd edn., Krishna prakshan Media Pvt. Ltd: Meerut; C-9, C-292, C-295. Beckett AH, Stenlake JB. 2007. Practical Pharmaceutical Chemistry. Part-II. 4th edn., CBS Publishers and Distributors: New Delhi; 85, 86.
3. Jeffery GH, Bassett J, Mondham J, Denney RC. 1989, Singapore; 5, 216-217., Hobart H
4. Willard, Lynne L Merritt, Jr., John A Dean, Frank A Settle, Jr. 1986. Instrumental
5. Methods of Analysis. 7th edn., CBS Publishers and Distributors: New Delhi; 1, 592, 622-628., Mendham J, Denney RC, Barnes JD, Thomas MJK. 2008, New Delhi; 29, 36,289-295.
6. International Conference on Harmonization, "Q2A: Text on Validation of Analytical Procedures," Federal Register 1995; 60(40):11260–11262.
7. Lloyd R Slyder, Joseph J Kirkland, Joseph L Glajch. 1997. Practical HPLC Method Development. 2nd edn. John Wiley and Sons, Inc., USA; 22-24, 42, 235-24.
8. Anonymous. www.ich.org.
9. Anonymous. 1994. ICH Harmonized Tripartite Guidelines, Text on Validation of Analytical Procedures: Text and Methodology. Q2A. Geneva; 1-8.
10. The United States of Pharmacopeia. 1995. 23/NF, 18, United States of Pharmacopeial Convention, inc., Rock Ville, MD., 1063,1961, 1988, 1990.
11. Gupta SC, Kapoor VK. 1996. Fundamentals of Mathematical Statistics. 9th edn. Sultan Chand and Sons: New Delhi; 2.6, 3.2-3.28.
12. FDA Guidance for industry, Analytical procedures and method validation (draft guidance), August 2000.
13. Szepesi G, 1989 Selection of High performance chromatographic methods in pharmaceutical analysis. J. Chromatograph. 464:265-278. Carr GP, Vahlich JC. 1990 A practical approach to method validation in pharmaceutical analysis, J. Pharmaceutical Biomedical Analysis, 86, 613-618.
14. https://www.drugbank.ca/drugs/DB01001,
Statistics
80 Views | 100 Downloads
How to Cite
Dadhich, B., Goyal, R., & Agarwal, D. (2021). Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Lansoprazole, Clarithromycin and Tinidazole in Combined Dosage Form. Asian Journal of Pharmaceutical Research and Development, 9(1), 102-109. https://doi.org/https://doi.org/10.22270/ajprd.v9i1.872