Lead Exposure Causes Alteration of Haematological Indices in Adult Female Wistar Rats

Authors

  • Adeyomoye O I Department of Physiology, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo City, Nigeria
  • Adewumi N A Department of Physiology, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo City, Nigeria.

DOI:

https://doi.org/10.22270/ajprd.v7i6.610

Keywords:

Lead, Environment, Toxicant, Haematological indices, Blood auto-analyzer

Abstract

Objective: Lead is a heavy metal widely distributed in the environment. It is known to be a toxicant and has no biological function. Humans are constantly exposed to lead from many sources and its effects on the haematological indices have not been fully elucidated. Therefore, this study aimed to investigate the changes in haematological profile of adult female Wistar rats exposed to lead.

Design: Forty-five female Wistar rats with average weight of 150 ± 20g were randomly distributed into 3 groups of 15 rats each. Group 1 served as the control and was exposed to water only, group 2 was exposed to lead acetate (500mg/L) while group 3 was exposed to lead acetate (1000mg/L). The rats were exposed to lead for 52 days. On the 26th, 39th and 52nd days of exposure, five rats (n=5) were sacrificed from each group after mild anaesthesia and their blood samples were taken into EDTA bottles for haematological analysis using blood auto-analyzer. Results were analyzed using two-way ANOVA and p<0.05 were considered statistically significant.

Main outcome measure: There was significant increase (p<0.05) in white blood cell and lymphocyte counts in 500mg/L and 1000mg/L lead exposed groups when compared to the control. There was significant decrease (p<0.05) in monocyte and granulocyte counts in 500mg/L and 1000mg/L lead exposed groups when compared to control group. Platelet counts significantly increased (p<0.05) in 500mg/L and 1000mg/L lead exposed groups when compared to control group.

Conclusion: In conclusion, exposure to lead at 500 and 1000mg/L increased white blood cells, lymphocyte and platelet counts while it decreased monocyte and granulocyte counts in adult female Wistar rats. Therefore, lead at the exposed levels caused some alterations in the haematological parametersin adult female Wistar rats.

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Author Biographies

Adeyomoye O I, Department of Physiology, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo City, Nigeria

Department of Physiology, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo City, Nigeria

Adewumi N A, Department of Physiology, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo City, Nigeria.

Department of Physiology, Faculty of Basic Medical Sciences, University of Medical Sciences, Ondo City, Nigeria.

References

1. Srinivasa-Reddy Y, Pullakhandam R, Radha Krishna KV, Uday Kumar P, Dinesh Kumar B.Lead and essential trace element levels in school children: a cross-sectional study. Ann Hum Biol. 2011; 38(3):372-7.
2. World Health Organization. Brief guide to analytical methods for measuring lead in blood 2011
3. Gagan-Flora F, Deepesh G, and Archana T. Toxicity of lead: A review with recent updates Interdiscip Toxicol. 2012; 5(2):47–58.
4. Centers for Disease Control and Prevention, U. S. Adult Blood Lead Epidemiology & Surveillance (ABLES). Retrieved 2017, https://www.cdc.gov/niosh/topics/ables/ description.html
5. Ab-Latif Wani, Anjum Ara, and Jawed Ahmad UsmaniLead toxicity: a review Interdiscip Toxicol. 2015; 8(2):55–64.
6. Deborah E. Keil, Jennifer Berger-Ritchie, Gwendolyn A. McMillinTesting for Toxic Elements: A Focus on Arsenic, Cadmium, Lead, and Mercury Laboratory Medicine, 2011; 42(12):735–742,
7. Alan A. Lead and children; Clinical management for family physicians; Can Fam Physician. 2010; 56(6):531–535.
8. Paul B Tchounwou, Clement G Yedjou, Anita K Patlolla, and Dwayne J Sutton. Heavy Metals Toxicity and the Environment. EXS. 2012; 101:133–164.
9. Agency for Toxic Substances and Disease Registry (ATSDR). Toxicological profile for lead. Atlanta,GA: US Department of Health and Human Services, 2007.
10. World Health Organisation. Lead poisoning and health. Fact sheet on Lead 2016, http://www.who.int/mediacentre/factsheets/fs379/en/
11. Michael A. Rieger and Timm Schroeder. Hematopoiesis Cold Spring Harb Perspect Biol. 2012; 4(12):a008250.
12. Debdatta Basu and Rajendra Kulkarni. Overview of blood components and their preparation. Indian J Anaesth. 2014; 58(5): 529–537.

13. Laura K. Vricella, Judette M. Louis, Edward Chien, and Brian M. Mercer. Am J Obstet Gynecol. 2015; 213(3):408.e1–408.
14. Nunyarat Koomkrong, Chaiwat Boonkaewwan, Watchara Laenoi, and Autchara Kayan Blood haematology, muscle pH and serum cortisol changes in pigs with different levels of drip lossAsian-Australas J Anim Sci. 2017; 30(12):1751–1755.
15. Adeyomoye O.I, Adewoye E.O Phytol Reduces Oxidative Stress and Cyclooxygenase-2 Expression in Kidney of Diabetic Wistar Rats Recent Advances in Biology and Medicine, 2018; 4(1):16-19.
16. Dyatlov V, Lawrence D, Neonatal lead exposure potentiates sickness behavior induced by Listeria monocytogenes infection of mice. Brain. behav. Imm, 2002; 4(16):477-92.
17. McCabe J, Berthiaume L, 1999. Functional roles for fatty acylated amino-terminal domain in subcellular localization. mol. bio.cell,1999; 11(10):3771-86.
18. Piomelli S, Childhood lead poisoning. Pediatr. Clin. North. Amer, 2002; 49:1285-1304.
19. Khan TA, Zafar F. Haematological Study in Response to Varying Doses of Estrogen in Broiler Chicken. International Journal of Poultry science, 2005; 4(10):748-751.
20. Barman T, Kalahasthi R, Rajmohan HR, Effects of lead exposure on the status of platelet indices in workers involved in a lead-acid battery manufacturing plant. J Expo Sci Environ Epidemiol. 2014; 24(6):629-33.
21. Adam SF, Laura MY, Joshua DB, Hong W, Patrick D, Kathleen AR, Ankita P, Mary AP, Braxton DM, Jeffrey RO, William H, Christopher RH, Jonathan DW, Joshua PL. Genetic variation in the platelet endothelial aggregation receptor 1 gene results in endothelial dysfunction. PLoS ONE 10(9): e0138795.

Published

2019-12-15

How to Cite

I, A. O., & A, A. N. (2019). Lead Exposure Causes Alteration of Haematological Indices in Adult Female Wistar Rats. Asian Journal of Pharmaceutical Research and Development, 7(6), 30–34. https://doi.org/10.22270/ajprd.v7i6.610

Issue

Vol. 7 No. 6 (2019): Vol 7 No 6 (2019): Volume 7 Issue 6 Nov - Dec. 2019

Section

Research Articles

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