Assessment of In-Vitro Antioxidant Potential of Ethyl Acetate Fraction of Hydroalcoholic Extract of Aerva Javanica Linn. Flowering Tops

Authors

  • Alok Khunteta LBS College of Pharmacy, Udai Marg, Tilak Nagar, Jaipur-302004, Rajasthan, India
  • Surendra K Swarnkar LBS College of Pharmacy, Udai Marg, Tilak Nagar, Jaipur-302004, Rajasthan, India
  • Manish Kumar Gupta School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, Jaipur-302017, Rajasthan, India
  • Aruna Swarnkar SMS Medical College, Jaipur-302004, Rajasthan, India
  • Pankaj Jain Department of Pharmacy, Banasthali Vidyapith, Banasthali, Newai, Rajasthan, India
  • Sarvesh Paliwal Department of Pharmacy, Banasthali Vidyapith, Banasthali, Newai, Rajasthan, India

DOI:

https://doi.org/10.22270/ajprd.v7i5.520

Keywords:

Aerva, SOD, superoxide, antioxidant, ascorbic acid, FRAP

Abstract

Aerva javanica (Amaranthaceae) is a grey coloured woolly perennial tomentose shrub. Its traditional and folklore usage motivates further investigation on its pharmacognostic parameters and pharmacological potential. Therefore, in order to establish its antioxidant potential, DPPH, SOD and superoxide scavenging and total antioxidant capacity, were determined. Hydro-alcoholic extract (CE) was prepared from flowering tops of A. javanica. In order to work further on activity guided fractions, ethyl acetate (AJEAF) fraction was prepared.  For in-vitro evaluation, ascorbic acid was used as standard antioxidant compound. In DPPH assay IC50 was determined as 89.00 µg/ml, as compared with standard ascorbic acid with IC50 21.80 µg/ml, with a concentration dependent scavenging of free radical. Superoxide scavenging potential in terms of SOD expressed as IC50, was determined as 61.904 µg /ml for AJEAF in contrast to 132.413 µg /ml for standard ascorbic acid. This was equivalent to 16.154 Eq SOD units /mg (EAF) per mg of sample respectively against 7.552 Eq SOD units /mg of standard. Total antioxidant capacity was found to be 283.67 mg Ascorbic acid Eq /g. Results indicated that fraction (AJEAF had significant antioxidant potential which expressed the prospective potential of fraction against metabolic disorders.

 

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Author Biographies

Alok Khunteta, LBS College of Pharmacy, Udai Marg, Tilak Nagar, Jaipur-302004, Rajasthan, India

LBS College of Pharmacy, Udai Marg, Tilak Nagar, Jaipur-302004, Rajasthan, India

Surendra K Swarnkar, LBS College of Pharmacy, Udai Marg, Tilak Nagar, Jaipur-302004, Rajasthan, India

LBS College of Pharmacy, Udai Marg, Tilak Nagar, Jaipur-302004, Rajasthan, India

Manish Kumar Gupta, School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, Jaipur-302017, Rajasthan, India

School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, Jaipur-302017, Rajasthan, India

Aruna Swarnkar, SMS Medical College, Jaipur-302004, Rajasthan, India

SMS Medical College, Jaipur-302004, Rajasthan, India

Pankaj Jain, Department of Pharmacy, Banasthali Vidyapith, Banasthali, Newai, Rajasthan, India

Department of Pharmacy, Banasthali Vidyapith, Banasthali, Newai, Rajasthan, India

Sarvesh Paliwal, Department of Pharmacy, Banasthali Vidyapith, Banasthali, Newai, Rajasthan, India

Department of Pharmacy, Banasthali Vidyapith, Banasthali, Newai, Rajasthan, India

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Published

2019-10-15

How to Cite

Khunteta, A., Swarnkar, S. K., Gupta, M. K., Swarnkar, A., Jain, P., & Paliwal, S. (2019). Assessment of In-Vitro Antioxidant Potential of Ethyl Acetate Fraction of Hydroalcoholic Extract of Aerva Javanica Linn. Flowering Tops. Asian Journal of Pharmaceutical Research and Development, 7(5), 133–136. https://doi.org/10.22270/ajprd.v7i5.520