Isolation and characterization of probiotics bacteria from curd, pickle and fermented rice and screening of antimicrobial activity
DOI:
https://doi.org/10.22270/ajprd.v7i2.481Keywords:
probiotics, antimicrobial activity, curd, fermented rice, pickle, biochemical characterizationAbstract
The live supplement of microorganisms though the dietary product is known as the probiotics. These are some special kind of bacteria they are healthy for the host organisms. These bacteria commonly found in food and dietary product. In our study, probiotics were isolated from fermented rice, curd and pickle .Total isolated strains were studied for their characterization, and also strains were studied antibacterial also their antibiotic susceptibility quality. The antibiotic resistance of potential strain was studied using Vancomycin, gentamycin, chloromphenicol, ciprofloxacin and cefataximide. PB1, PB3 shows the good resistance activity to the antibiotics. The curd produced by those strains has good quality and quantity of nutrients. Specially this study is for isolating, identifying, and characterizing the probiotics .Isolated strains were identified by Gram staining, catalase assay, and 3 molecular identification methods; namely, (GTG) 5-PCR fingerprinting, This experiments shows the better anti-pathogen activity, and acceptable antibiotic susceptibility; it implies we can use these probiotics in different purpose in food industry for modern food synthesis, for antimicrobial susceptibility test we are taking amplicin, karamycin, tetracycline, tripsin by using of the antibiotics concluded that various inhibition zones of given sample of probiotics.
Downloads
References
2. Buckenhuskes HJ selection criteria for lactic acid bacteria to be used as starter culture for the various food commodities FEMS microbiology Rev. 1993; 12:253-272.
3. Fuller R, Newport MJ 1987, the effect of diluted yogurt on the gut microbiology and growth of piglets, food microbiology 4:83-85.
4. Fujisawa T, watabe J, Mitsuoka T lactobacillus aviaries spp, a new species isolated from the curd, system application microbiology. 1984; 5:414-420.
5. Fuller R Probiotic in man and animal: a review J application bacterial. 1989; 66:365-378.
6. Graciela FV, Maria PT, food microbiology protocols , Totowa new jersey, human press inc. chap.21, probiotic properties of lactobacillus 2001; 173-181.
7. MC farland LV, Beneficial microbes health or hazard, eur J gastroentrol hepatol 2005; 12:1069- 1071.
8. Mahantesh m patil, Ajay pal, T Anand and K V ramana, isolation characterization of lab from curd and cucumber, Indian journal of biotechnology, 2010; 9:166-172.
9. Reuter G, klein G and Goldberg m, identification of Probiotics culture feed sample, food research international 2004; 35 : 117-124.
10. Parvathy seema nair Biochemical characterization of lab isolated from fish and prawn, journal of culture collection 2005; 44: 48-52.
11. Sarvedra JM, Abi heena and moore A long term consumption of infant formula containing live Probiotics tolerance and safety, American journal clinical research 2008; 79: 261-67.
12. Keeney KM, Finlay BB, effect of antibiotic on human microbiota and subsequent disease, annu. Rev. Microbiology 2014; 68: 217-35
13. Croswell A, salzman NH the impact of antibiotic on microbial ecology to enteric salmonella infection immune. 2009; 77: 2741-53
14. Felis GE, Dellagilo f. taxonomy of lactobacillus and bifidobacteria. Current issue in intestinal microbiology 2007; 8: 44-61
15. Joint WHO working group report on drafting guideline for the evaluation of Probiotics in food Ontario: 2000
16. Saarela M, Mongensen G fonden R probiotic safety functional and technological properties journal of biotechnology 2000: 84(3): 197- 215
17. Kosin B criteria for production of probiotic, food technology and biotechnology. 2006; 44(3): 371- 379
18. Lahtinen SJ probiotic viability microbiology ecology in health and disease, 2012; 23: 10 -14
19. Salminen S, Benno y, Lee YK probiotic trends in food science and technology. 1999; 10(3): 107-110
20. Dysbiosis in infants is an important environmental factor of development origin of health and disease page: 43-54
21. Beneficial effects of Probiotics for infants and children with dysbiosis. Page 24-27
22. Bernardo petriz, metaproteomic approach to gut microbiota in health and disease pages: 58-78
23. Probiotics and prebiotics, current research and trends , koen venema and paula do carmo. Pages; 34-45
24. Latic acid bacteria and bifidobacteria current progress in advance research, kenji sonomoto and atsushi yokota, pages 45-65
25. Venketeshwar and Leticia rao, Probiotics and prebiotics in human health and nutrition. 2007, pages: 34-67
26. Clinical infection disease and Probiotics benefits volume 45 February2007 pages 58-62
27. Yuan kun lee, baltasar mayo, basic fundamental of probiotic microorganism 14 july 2008, pages: 23-45.
Published
How to Cite
Issue
Section
AUTHORS WHO PUBLISH WITH THIS JOURNAL AGREE TO THE FOLLOWING TERMS:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).