Protective Effects of Ruta Chalepensis L. (Rutacae) Oil Extract Against Potassium Bromate Induced Nephrotoxicity in Male Rats: Histopathological Study

Authors

  • Ibrahim S. Eldurssi Zoology Department, Science Faculty, Omar Al-mukhtar University, El-Beida-Libya,P.O.BOX 919 - El-Beida-Libya
  • Ebtesam MM Zoology Department, Science Faculty, Omar Al-mukhtar University, El-Beida-Libya,P.O.BOX 919 - El-Beida-Libya
  • Gasem MA Gheth Zoology Department, Science Faculty, Omar Al-mukhtar University, El-Beida-Libya,P.O.BOX 919 - El-Beida-Libya
  • Abdullah AH Abdalla Zoology Department, Science Faculty, Omar Al-mukhtar University, El-Beida-Libya,P.O.BOX 919 - El-Beida-Libya
  • Mabroka A Algassi Zoology Department, Science Faculty, Omar Al-mukhtar University, El-Beida-Libya,P.O.BOX 919 - El-Beida-Libya

DOI:

https://doi.org/10.22270/ajprd.v7i2.466

Keywords:

Rats, Kidney, Potassium Bromate, Ruta chalepensis, Histopathology

Abstract

Ruta chalepensis L. (Rutaceae) is a perennial herb, widely distributed in the Mediterranean area, with glabrous stem, alternate bipinnatisect leaves. It is an ancient medicinal plant still being used in the traditional medicine of many countries. Potassium bromate (KBrO3) is a strong oxidizing agent that has been used in flour milling, as a cheese making, in beer malting, as a component of cold hair-wave liquids. This study studies the protective and curative effects of R. chalepensis oil extract against KBrO3 toxicity on kidney of male rats. Accordingly, a total number of fifty male albino rats were divided into five groups. The first group served as a control animals. The second group was administered Rue at an oral daily dose of 0.5 g/Animal for four weeks. The third group received KBrO3 100 mg/kg/b. w. for four weeks. The fourth group (protective group) was initially administered with Rue alone for 2 weeks and followed by KBrO3 in association with Rue for 2 weeks. The fifth group (therapeutic group) was first given KBrO3 alone for 2 weeks and was secondly administered Rue in association with KBrO3 for 2 weeks. At the end of 2nd  and 4th  weeks of treatment, the kidney tissues were collected for histopathological studies. Histopathological studies revealed that the renal reactions of rats from the Rue group showed normal architecture pattern same that observations as in the control rats. The rats treated with KBrO3 showed vacuolation and contraction of glomerular tuft, separation in intertubular connective tissue, necrotic changes in some areas and hyaline changes in tubules. Furthermore, lymphoid infiltration and intratubular hemorrhage, dilated in tubules with loss of cellular boundary. In the protective group minimal changes in the structure of the kidney was revealed compared to control. Both glomeruli and convoluted tubules gained the normal features. In therapeutic group, renal sections revealed improvement in the histological structure though there was a mild necrosis and slight in inflammatory leucocytic infiltration. The results of this study suggest the nephrotoxic effect of KBrO3 and the ameliorative effect of Rue when administrated for protective and therapeutic purposes.

 

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Author Biographies

Ibrahim S. Eldurssi, Zoology Department, Science Faculty, Omar Al-mukhtar University, El-Beida-Libya,P.O.BOX 919 - El-Beida-Libya

Zoology Department, Science Faculty, Omar Al-mukhtar University, El-Beida-Libya,P.O.BOX 919 - El-Beida-Libya

Ebtesam MM, Zoology Department, Science Faculty, Omar Al-mukhtar University, El-Beida-Libya,P.O.BOX 919 - El-Beida-Libya

Zoology Department, Science Faculty, Omar Al-mukhtar University, El-Beida-Libya,P.O.BOX 919 - El-Beida-Libya

Gasem MA Gheth, Zoology Department, Science Faculty, Omar Al-mukhtar University, El-Beida-Libya,P.O.BOX 919 - El-Beida-Libya

Zoology Department, Science Faculty, Omar Al-mukhtar University, El-Beida-Libya,P.O.BOX 919 - El-Beida-Libya

Abdullah AH Abdalla, Zoology Department, Science Faculty, Omar Al-mukhtar University, El-Beida-Libya,P.O.BOX 919 - El-Beida-Libya

Zoology Department, Science Faculty, Omar Al-mukhtar University, El-Beida-Libya,P.O.BOX 919 - El-Beida-Libya

Mabroka A Algassi, Zoology Department, Science Faculty, Omar Al-mukhtar University, El-Beida-Libya,P.O.BOX 919 - El-Beida-Libya

Zoology Department, Science Faculty, Omar Al-mukhtar University, El-Beida-Libya,P.O.BOX 919 - El-Beida-Libya

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Published

2019-04-15

How to Cite

Eldurssi, I. S., MM, E., Gheth, G. M., Abdalla, A. A., & Algassi, M. A. (2019). Protective Effects of Ruta Chalepensis L. (Rutacae) Oil Extract Against Potassium Bromate Induced Nephrotoxicity in Male Rats: Histopathological Study. Asian Journal of Pharmaceutical Research and Development, 7(2), 88–92. https://doi.org/10.22270/ajprd.v7i2.466

Issue

Vol. 7 No. 2 (2019): Volume 7 Issue 2 March - April 2019

Section

Research Articles

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