Screening of Selected Sri Lankan Seasonal Fruit Extracts for Total Antioxidant Activity in vitro

Authors

  • Amarosige Shanoli Kavindya Fernando Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, University of Ruhuna, Galle, Sri Lanka
  • Anoja Priyadarshani Attanayake Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka.
  • Kamani Ayoma Perera Wijewardena Jayatilaka Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka.

DOI:

https://doi.org/10.22270/ajprd.v7i1.459

Keywords:

antioxidant assays, FRAP assay, DPPH assay, nutraceuticals, Sri Lankan fruit extracts, TBA assay

Abstract

The present study aimed to determine the total polyphenol content and total antioxidant activities of ten selected seasonal fruit extracts grown in Sri Lanka. The aqueous refluxed fruit extracts (4h) were used at the initial concentration of 0.05 g/mL. The total polyphenol content was determined according to the Folin-Ciocalteu method. The total antioxidant activity was evaluated by DPPH (2, 2-diphenyl-2-picrylhydrazyl), FRAP (ferric reducing power) and TBA (thiobabituric acid assay) assays with L-ascorbic acid as the reference compound. The total polyphenol content of the fruit extracts varied from 0.81±0 to 17.54±0.50 mg GAE (gallic acid equivalents) per gram of dry weight. The antioxidant activities ranged in IC50 of 46.60±0.60 to 367.90 ± 4.90 μg/mL, 3.41±0.02 to 50.46±1.02 µM, 30.62±0.50 to 182.64±0.90 mg AAE (L-ascorbic acid equivalents) per gram of dry weight for DPPH, FRAP, TBA assays, respectively. All aqueous fruit extracts exert dose dependent in vitro antioxidant activities in different degrees. Among the selected fruit extracts S. carophylatum, P. zeylanica, A. comosus exert relatively high total antioxidant activity together with high total polyphenol content. Hence, the selected fruit extracts are deserved to be as potent sources for antioxidant lead compounds in the development of nutraceuticals using the above edible fruits.

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Author Biographies

Amarosige Shanoli Kavindya Fernando, Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, University of Ruhuna, Galle, Sri Lanka

Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, University of Ruhuna, Galle, Sri Lanka

Anoja Priyadarshani Attanayake, Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka.

Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka.

Kamani Ayoma Perera Wijewardena Jayatilaka, Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka.

Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka.

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Published

2019-02-15

How to Cite

Fernando, A. S. K., Attanayake, A. P., & Jayatilaka, K. A. P. W. (2019). Screening of Selected Sri Lankan Seasonal Fruit Extracts for Total Antioxidant Activity in vitro. Asian Journal of Pharmaceutical Research and Development, 7(1), 7–12. https://doi.org/10.22270/ajprd.v7i1.459