RP-HPLC Method Development and Validation for Estimation of Acebrophylline
DOI:
https://doi.org/10.22270/ajprd.v6i6.445Keywords:
HPLC, RP-HPLCAbstract
Chromatography, a separation technique, is mostly used in chemical analysis in which High-performance liquid chromatography (HPLC) is an extremely versatile technique where analytes are separated by passage through a column packed with micrometer-sized particles. Theses day reversed-phase chromatography is commonly used separation technique in HPLC. The reasons for this include the simplicity, versatility, and scope of the reversed-phase method as it is able to handle compounds of a diverse polarity and molecular mass. Reversed phase chromatography has found both analytical and preparative applications in the area of biochemical separation and purification. Molecules that possess some degree of hydrophobic character, such as proteins, peptides and nucleic acids, can be separated by reversed phase chromatography with excellent recovery and resolution. This review covers the importance of RP-HPLC in analytical method development and their strategies along with brief knowledge of critical chromatographic parameters need to be optimized for an efficient method development.
Key Words- HPLC, RP-HPLC
Downloads
References
2. Liu Y., Lee M.L. Ultrahigh pressure liquid chromatography using elevated temperature. Journal of Chromatography. 2006; 1104 (1-2): 198–202.
3. Abidi,S.L. High-performance liquid chromatography of phosphatidic acids and related polar lipids. J. Chromatogr. 1991; 587: 193-203.
4. Hearn M.T.W. Ion-pair chromatography on normal and reversed-phase systems. Adv. Chromatogr. 1980; 18:59–100.
5. Bergh J. J., Breytenbach, J. C. Stability-indicating High-performance Liquid- chromatographic Analysis of Trimethoprim in Pharmaceuticals. J.Chromatogr. 1987; 387: 528-531.
6. Stubbs C., Kanfer, I. Stability-indi-cating High-performance Liquid-chromato- graphic Assay of Erythromycin Estolate in Pharmaceutical Dosage Forms. Int. J.Pharm. 1990; 3(2): 113-119.
7. MacNeil L., Rice J. J., Muhammad N. Lauback R. G. Stability-indicating Liquid-chromatographic Determination of Cefapirin, Desacetylcefapirin and Cefapirin Lactone in Sodium Cefapirin Bulk and Injectable Formulations. J. Chromatogr. 1986; 361: 285-290.
8. Chromatographic Stability-indicating Determination of Zopiclone in Tablets. J. Chromatogr. 1994; 677(1): 87-93.
9. Lauback R. G., Rice J. J., Bleiberg B., Muhammad N., Hanna, S. A. 1984. Specific High-performance Liquid-chromato- graphic Determination of Ampicillin in Bulks, Injectables, Capsules and Oral Suspensions by Reversed-phase Ion-pair Chromatography. J. Liq. Chromatogr. 1984; 7(6): 1243-1265.
10. Wiklund A E., Dag B., Brita S. Toxicity evaluation by using intact sediments and sediment extracts. Marine Pollution Bulletin (2005); 50(6): 660-667.
11. Kwok Y. C., Hsieh D. P. H., Wong P.K. Toxicity identification evaluation (TIE) of pore water of contaminated marine sediments collected from Hong Kong waters. Marine Pollution Bulletin. 2005; 51(8-12): 1085-1091.
12. Hongxia Yu., Jing C., Cui Y., Shang H., Ding Z., Jin H. Application of toxicity identification evaluation procedures on wastewaters and sludge from a municipal sewage treatment works with industrial inputs. Ecotoxicology and Environmental Safety. 2004; 57(3): 426-430.
13. Ayerton J. Assay of ceftazidime in biological fluids using high-pressure liquid chromatography. J. Antimicrob. Chemother.1981; 8: 227-231.
14. Bowden R.E., Madsen P.O. High- pressure liquid chromatographic assay of sulbactam in plasma, urine and tissue. Antimicrob.Agents Chemother.1986; 30: 31-233.
15. Haginaka J., Yasuda H., Uno T., Nkagawa T. Alkaline degradation and determination by high-performance by high-performance liquid chromatography. Chem. Pharm. Bull. 1984; 32: 2752-2758
16. Fredj G., Paillet Aussel M. F., Brouard A., Barreteau H., Divine C., Micaud M. Determination of sulbactam in biological fluids by high-performance liquid chromatography. J. Chromatogr. 1986; 383: 218-222.
17. Rodenas V., Garcia M.S., Sanchez-Pedreno C., Albero M.I. Flow-injection spectrophotometric determination of frusemideorsulphathiazole in pharmaceuticals. J. Pharm. Biomed. Anal.1997; 15: 1687-1693.
18. Shah A.J., Adlard M.W., Stride J.D. A sensitive assay for clavulanic acid and sulbactam in biological fluids by high-performance liquid chromatography and pre column derivatization. J. Pharm. Biomed. Anal.1990; 5: 437-443.
19. Abidi S.L. High-performance liquid chromatography of phosphatidic acids and related polar lipids. J. Chromatogr. 1991; 587: 193-203.
20. Christie W.W., Gill S., Nordbäck J., Itabashi Y., Sanda S., Slabas A.R. New procedures for rapid screening of leaf lipid components from Arabidopsis. Photochemical Anal.1998; 9: 53-57.
21. Sethi PD. In High Performance Liquid Chromatography Quantitative analysis of Pharmaceutical formulations, New Delhi: CBS Publishers & Distributors, 1997,p 173-210
Published
How to Cite
Issue
Section
AUTHORS WHO PUBLISH WITH THIS JOURNAL AGREE TO THE FOLLOWING TERMS:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
.