ETHANOL EXTRACT AND ACTIVE FRACTION EFFECT OF Moringa Oleifera. Lam IN INHIBITING COX-2 ACTIVITY ON MCF-7 CELL

Authors

  • Azizah D 1Graduate Student, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155 Indonesia
  • Masfria K Graduate Student, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155 Indonesia.
  • Poppy AZH. 3Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155 Indonesia

DOI:

https://doi.org/10.22270/ajprd.v6i4.399

Keywords:

Moringa leaf, cancer cells MCF-7, Apoptosis and COX-2

Abstract

The expression of COX-2 is involved in carcinogenesis through the proliferative process, angiogenesis and inhibition of apoptosis. Moringa oleifera has been shown to function as an anti-oxidant, anti-inflammatory, anti-microbial and anticancer. Moringa leaf extraction was done by percolation method, and continued to liquid-liquid extraction to obtain nonpolar, semipolar, and polar fractions using n-hexane and ethylacetate solvent.The aim of this study was to determine the effect of ethanol extract and the fraction of n-Hexane of  Moringa oleifera  leaf in decreased MCF-7 breast cancer cell and COX-2 expression. This study used true experimentation in vitro design using MCF-7 cells. MTT Assay was performed using a therapeutic dose 6,125-1000 μg / ml to determine IC50 in MCF-7 cells. IC50 of ethanol extract was  obtained  94.44 μg / ml and  97.60 μg / ml for n-Hexane fraction. Selectivity index of both samples was 3.95 μg / ml and 2.87 μg / ml. The expression of COX-2 was tested qualitatively with immunocytochemical methods and showed that ethanol extract and n-hexane fraction of Moringa oleifera leaf were able to suppress COX-2 enzyme expression on MCF-7 cells depending on concentration.

Keywords: Moringa leaf, cancer cells MCF-7, Apoptosis and COX-2

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Published

2018-08-23

How to Cite

D, A., K, M., & AZH., P. (2018). ETHANOL EXTRACT AND ACTIVE FRACTION EFFECT OF Moringa Oleifera. Lam IN INHIBITING COX-2 ACTIVITY ON MCF-7 CELL. Asian Journal of Pharmaceutical Research and Development, 6(4), 1–5. https://doi.org/10.22270/ajprd.v6i4.399

Issue

Vol. 6 No. 4 (2018): VOLUME 6 ISSUE 4 JULY - AUGUST 2018

Section

Research Articles

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