ANALYTICAL METHOD DEVELOPMENT, VALIDATION AND FORCED DEGRADATION STUDIES OF LANSOPRAZOLE BY RP-HPLC

Authors

  • Madhavi K Meher Department of Pharmaceutical Analysis, PRES’s , College of Pharmacy, Chincholi, Nasik. M.S. India
  • Charushila Bhangale Department of Pharmaceutics,PRES’s ,College of Pharmacy, Chincholi, Nasik, M.S. India
  • Ramdas Dholas Department of Pharmacognosy, PRES’s , College of Pharmacy, Chincholi, Nasik. M.S. India
  • Vandana Aher Prashant Department of Medicinal Chemistry, Pravara Rural College of Pharmacy, Loni, M.S, India
  • Rohan Pawar RAP Analytical Research and Training Centre , Nasik , M.S, India

DOI:

https://doi.org/10.22270/ajprd.v6i4.387

Keywords:

Lansoprazole, RP-HPLC, Chromatogram, validation, estimation.

Abstract

The objective of this work is to develop a rapid, precise, accurate and sensitive revere phase liquid chromatographic method and Forced degradation studies for the estimation of Lansoprazole. The chromatographic method was standardized for Lansoprazole using Shimadzu HPLC model reverse phase analytical Inspire grace C18 column (250 mm x 4.5 mm, 5mm particle size) with PC-3000-M Reciprocating Pump (40 Mpa) and UV-3000-M Detector, at 285nm and flow rate of 0.8 ml/min. The mobile phase consists of 80:20 Methanol: water. The linearity of proposed method was investigated in the range of 10-50 µm/ml (R2 = 0.999) of Lansoprazole. The limit of detection (LOD) was found to be 0.12 mm/ml. The limit of quantification (LOQ) was found to be 0.36 mg/ml. The retention time of Lansoprazole found to be 5.4 min. The method was statistically validated and % RSD was found to be less than 2 indicating high degree of accuracy and precision. Hence proposed method can be successfully applied for the estimation of Lansoprazole in further studies.

Keywords: Lansoprazole, RP-HPLC, Chromatogram, validation, estimation.

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Published

2018-08-23

How to Cite

Meher, M. K., Bhangale, C., Dholas, R., Prashant, V. A., & Pawar, R. (2018). ANALYTICAL METHOD DEVELOPMENT, VALIDATION AND FORCED DEGRADATION STUDIES OF LANSOPRAZOLE BY RP-HPLC. Asian Journal of Pharmaceutical Research and Development, 6(4), 21–29. https://doi.org/10.22270/ajprd.v6i4.387