In silico and in vitro testing Ascorbic Acid Protects Nicotine-Treated Human Erythrocytes
Objectives: Nicotine is a naturally occurring drug that is highly addictive and is commonly found in tobacco products. Tobacco products are one of the leading causes of lung and oral cancer worldwide. Because blood is the primary organ in contact with nicotine, our goal in this study was to confirm its effect on blood. We also investigated the protective role of ascorbic acid on nicotine toxicity.
Methods: Various blood toxicity studies were carried out using in-vitro and in-silico methods. Nicotine-induced haematological perturbation resulted in haemolysis, decreased superoxide dismutase and catalase activities, and decreased total antioxidant capacity in erythrocytes.
Results and Conclusions: The results showed that nicotine has a negative effect on red blood cells in the lysis assay and causes clots to form in nicotine-treated samples in the blood clotting analysis. Furthermore, the in-Silico method validated the in-vitro results. According to our findings, ascorbic acid has blood-protective properties. Ascorbic acid was discovered to increase SOD, catalase, and total antioxidant activity. Ascorbic acid also reduced the damage caused by nicotine to RBCs in the lysis assay and demonstrated a high level of protection against the formation of clots when the samples were treated with nicotine. The findings suggest that ascorbic acid, an antioxidant, can protect against nicotine-induced haematological damage.
2. Mishara,A, et al. . Harmful effects of nicotine. Indian J Med Paediatr Oncol, 2015; 36(1), 24–31.
3. Thompson, J., et al. Development of screening assays for nanoparticle toxicity assessment in human blood: preliminary studies with charged Au nanoparticles. Nanomedicine, 2012; 7(9)1355–1364.
4. Uchida S and Kagitani F. Effects of nicotine on regional blood flow in the olfactory bulb in response to olfactory nerve stimulation. The Journal of Physio. Sci., 2020; 70(1)1-10.
5. Mongi S. et al. Protective effects of vitamin C against haematological andbiochemical toxicity induced by deltamethrin in male Wistar rats. Ecotoxicol Environ Saf, 2011; 74(6)1765-1769.
6. Jacobs MH. Osmotic properties of the erythrocyte. III. The applicability of osmoticlaws to the rate of hemolysis in hypotonic solutions of non-electrolytes. Biol Bull,1932; 62(2)178–194.
7. Li YQ, Hu R, Zhong LH, et al. Synergistic effect of trehalose and saccharose pretreatment on maintenance of lyophilized human red blood cell quality. Trop J Pharm Res 2016; 15(3):527–533.
8. Nishikimi M, Rao NA and Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem BiophysRes Commun, 1972; 46(2):849–854.
9. Aebi H.Catalase. Meth Enzymol 1984; 105: 121–126.
10. Aryal S. (2021). Catalase test- principle, uses, procedure, result interpretation with precautions. Retrieved from https://microbiologyinfo.com/catalase-test-principleuses-procedure-result-interpretation-with-precautions/
11. Islam, M., Aryasomayajula, A. and Selvaganapathy, P. A Review on Macroscale and Microscale Cell Lysis Methods, Micromachines , 2017;8(3)83-90.
12. Koracevic, D, Koracevic. G. et al, Total Antioxidant Capacity. J Clin Pathol, 2001;54, 356-361
13. Li YQ, Hu R, Zhong LH, et al. Synergistic effect of trehalose and saccharose pretreatment on maintenance of lyophilized human red blood cell quality. Trop J Pharm Res 2016; 15(3):527–533.
14. Berman HM. The Protein Data Bank: a historical perspective. Acta Crystal Section A: Foundations of Crystallography 2008; 64(1)88-95.
15. Bates S and Weitz, J. Coagulation assays. Circulation, 2005; 112(4)53-60.
16. Vishnuvarthan VJ, Lakshmi KS & Srividya AR. In-silico screening of flavonoids targeted for death receptors in cancer by using Hex molecular docking. Journal of Young Pharmacists, 2017; 9(2)168.
17. Lo, J. O., Schabel, M. C., Roberts, V. H., Morgan, T. K., Rasanen, J. P., Kroenke, C. D., Shoemaker, S. R., Spindel, E. R., & Frias, A. E.. Vitamin C supplementation ameliorates the adverse effects of nicotine on placental hemodynamics and histology in nonhuman primates. Am. J. Obstet. Gynecol,; 2015 :212(3)
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