Phytochemicals of Extract N-Hexane Leaves Bangun-Bangun (Plectranthus Amboinicus) (Lour.) Spreng and Antibacterial Activity Causes Diarrhea

  • Nurbaiti Br. Singarimbun Departemen of Midwifery, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia
  • Defacto Firmawati Zega Departemen of Midwifery, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia
  • Defacto Firmawati Zega Departemen of Midwifery, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia
  • Helen Anjelina Simanjuntak Departemen of Pharmacy, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia
  • Hermawan Purba Departemen of Pharmacy, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia
  • Kasta Gurning Departemen of Pharmacy, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia

Abstract

Objective: This research aims to determine the secondary metabolites contained in the n-hexane extract of P. ambionicus leaves and to determine the effective concentration as an antibacterial causing diarrhea.


Methods: The research method was carried out experimentally with the stages of making simplicia, maceration, extract making, phytochemical screening, and antibacterial activity testing.


Results: The results of phytochemical screening showed the presence of flavonoids, saponins, tannins, steroids/triterpenoids. The results of the antibacterial activity test showed that the n-hexane extract of P.amboinicus leaves at all concentrations (50%, 25%, 12.5%) had antibacterial activity in the strong category (10-20 mm).


Conclusion: The n-hexane extract of P.amboinicus leaves has the potential as an antibacterial with a strong category.


 

Keywords: Phytochemicals, Extract, P.amboinicus, Antibacterial, Diarrhea

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Author Biographies

Nurbaiti Br. Singarimbun, Departemen of Midwifery, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia

Departemen of Midwifery, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia

Defacto Firmawati Zega, Departemen of Midwifery, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia

Departemen of Midwifery, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia

Defacto Firmawati Zega, Departemen of Midwifery, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia

Departemen of Midwifery, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia

Helen Anjelina Simanjuntak, Departemen of Pharmacy, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia

Departemen of Pharmacy, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia

Hermawan Purba, Departemen of Pharmacy, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia

Departemen of Pharmacy, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia

Kasta Gurning, Departemen of Pharmacy, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia

Departemen of Pharmacy, Sekolah Tinggi Ilmu Kesehatan Senior Medan, Indonesia

References

1. Barkatullah BB., Muahmmad I., Niaz A., Naveed M and Rehmanullah. Antispasmodic potential of leaves, barks and fruits of Zanthoxylum armatum DC. African Journal of Pharmacy and Pharmacology. 2013; 7(13):685-693.
2. Gurning K., R. Boangmanalu., H.A. Simanjuntak., N.Singarimbun., R. Rahmiati and W. Lestari. Identification of Secondary Metabolites and Antidiarrheal Activity of Pirdot Leaves Ethanol Extract (Saurauia vulcani Korth.) From West Pakpak North Sumatera Province Indonesia. Rasayan J. Chem. 2020; 13(4):2385-2389.
3. Depkes RI. Target Tujuan Pembangunan MDGs. Direktorat Jenderal Kesehatan Ibu dan Anak. 2011. Jakarta.
4. Bonten M., James RJ., Anita HJ Van de B., Leonida G., Jeroem G., Patricia I de P., Stefan G., Thomas V., Peter H and Jan TP. Epidemiology of Escherichia coli Bacteremia: A Systematic Literature Review. Clinical Infectious Diseases. 2019; 1211-1219.
5. Sarowska J., Bozena FK., Agnieszka JK., Magdalena FM., Marta K., Gabriela BP and Irena CK. Virulence factors, prevalence and potential transmission of extraintestinal pathogenic Escherchia coli isolated from different source: recent reports. BMC. 2019; 11:10
6. Levinson W., Peter CH., Elizabeth A.J., Jesse N and Brian S. Review of Medical Microbiology & Imunologi. 2018. Fiftheenth Edition.McGraw-Hill Education. United States.
7. Tuhin RH., Mst. Marium B., Md. Sohanur R., Rubaba K., Taslima B., Siraj UA., Ronia M., Amir H., Mohamed A-D., and Rayhana B. Wound Healing Effect of Euphorbia hirta Linn. (Euphorbiaceae) in Alloxan Induced Diabetic Rats. BMC Complementary and Alternative Medicine. 2017; 17: 423.
8. Singh A., Anamika D., Diksha P., Om P., Ravendra K., Rishendra K and AK Pant. Methyl nonyl ketone and linalool rich essential oils from three accessions of Zanthoxylum armatum (DC.) and their biological activities. International Journal of Herbal Medicine. 2019; 7(3): 20-28.
9. El-hawary S S., Rabie H E., Azza R A., Rehab S A and Amany A S. Polyphenolics content and biological activity of Plectranthus amboinicus (Lour.) Spreng growing in Egypt (Lamiaceae). PHCOG J. 2012; 4(32):45-54
10. Oliveira F F M D., Alba F T., Thially B G., Gilvandete M P S., Cibele B M C., Milena B A., Lilia M C C., Silvia H R., Alice M C M., Jose T V J and Aparecida T N D. Efficacy of Plectranthus amboinicus (Lour.) Spreng in a Murine Model of Methicillin-Resistant Staphylococcus aureus Skin Abscesses. Evidence-Based Complementary and Alternative Medicine. 2013. Hindawi Publishing Corporation.
11. Bhatt P and Pradeep S N. Antioxidant and Antibacterial Activities in the Leaf Extracts of Indian Borage (Plectranthus amboinicus). Food and Nutrition Science. 2012; 3:146-152
12. Gupta S., Praveena B., Gilbert S J., Pradeep SN., Mandyam C V. Phenolic constituents and biological activities of leaf extract of traditional medicinal plant Plectranthus amboinicus Benth (Lamiaceae). Tang Humanitas Medicine. 2013; 3(4):e32.
13. Asiimwe S., Anna K B K., Muhammad A., Kamatenesi MM., Agnes N and Ndukui J G. Chemical composition and toxicological evaluation of the aqueous leaf extracts of Plectranthus amboinicus Lour. Spreng. International Journal of Pharmaceutical Science Invention. 2014; 3(2):19-27.
14. Hassani MS., I Z., S Z and M Oukessou. Chemical composition and antimicrobial activity of Plectranthus amboinicus (Lour) Spring. Essential Oil from Archipelago of comoros. Journal of Essential oil Bearing Plants. 2012; 15(4):637-644.
15. Arumugam G., Mallappa KS and Uma RS. Plectranthus amboinicus (Lour.) Spreng: Botanical, Phytochemical, Pharmacological and Nutritional Significance. Molecules. 2016; 21.369.
16. Ruan TZ., CL Kao., Y L. Hsieh., H T Li and C Y. Chen. Chemical constituens of the leaves. Chemistry of natural Compounds. 2019; 55(1):124-126.
17. Zhang J Y P., Kao C L., Tsai Y.S., Wu MD., Cheng MJ., Chen C.Y and Liu SL. A New Homomonoterpenoid of Plectranthus amboinicus. Chemical & Pharmaceutical Research. 2022; 4(1):1-3.
18. K Jiyauddin., Samer AD., Darashhni T., Rasha S., Jawad A., M.Kaleemullah., S Budiasih, Rasny MR., M. Qamar., Hamid K., Sakina R., Junainah AH., Fadli A and Eddy Y. Comparison of antibacterial activity of Ocimum tenuiflorum L and Plectranthus amboinicus (Lour). Spreng Against the clinical pathogens Staphylococcus aureus, Pseudomonas aeruginosa and Escherchia coli. World Journal of pharmaceutical Research. 2015; 4(3):1887-1901
19. Simanjuntak, H A. Antibacterial Activity Extract of Kitolod (Hippobroma longiflora) Leaf Against Staphylococcus aureus and Salmonella typhi. Asian Journal of Pharmaceutical Reseacrh and Development. 2020; 8(1):52-54.
20. Musila FM., Joseph MN., Catherine WL and Saifuddin FD. Antibacterial and antifungal activities of 10 Kenyan Plectranthus species in the Coleus clade. Journal of Pharmacy Research. 2017; 11(8):1003-1014.
21. Silitonga M., Syafruddin I., Salomo H and Herbert Sipahutar. Levels of Apigenin and Immunostimulatory Activity of Leaf Extracts of Bangunbangun (Plectranthus amboinicus Lour). International Journal of Biology. 2015; 7(1):46-53)
22. Tajuddin Y., Muhammad H Y and Diveendrran Menon A/L Ravinagaraja M. Preliminary Antimicrobial Study of Plectranthus amboinicus stem and leaf extract from malacca. KPJ Medicinal Journal 8. 2020; 39-42)
23. Slusarczyk S., Adam C., Yulianri RY., Malgorzata SS., Zora V., Marta S., Dorota W and Adam M. Phytochemical profile and antioxidant activities of Coleus amboinicus Lour. Cultivated in Indonesia and Poland. Molecules. 2021; 26.2915.
24. Muzafri A., E Julianti and H.R. The extraction of antimicrobials component of andaliman (Zanthoxylum acanthopodium DC.) and its application on catfish (Pangasius sutchi) fillet. IOP Conf. Series: Earth and Environment Science 122 (2018).
25. Amelia S., NDA Lubis., R Balatif., MF Rozi and S P Sidhi. Antibacterial effect of Andaliman (Zanthozylum acanthopodium) against contaminant in raw common carp (Cyprinus carpio Linnaeus). IOP Conf. Series: Earth and Environmental Science. 2019; 425 (2020).
26. Sepriani O., Nurhamidah dan Dewi H. Potensi Ekstrak Tumbuhan Andaliman (Zanthoxylum acanthopodium DC.) Sebagai Antibakteri Staphylococcus aureus. ALOTROP. Jurnal Pendidikan dan Ilmu Kimia. 2020; 4(2):133-139.
27. Pratiwi D., Irma S dan Mariyam A. Efek antibakteri ekstrak kulit jeruk nipis (Citrus aurantifolia) Terhadap Salmonella typhi secara in vitro. Saintika Medika. 2014; 9(2):110-115.
28. (Simanjuntak, H A and Rahmiati. Antibacterial and Antifungal Activities of Patikan Kebo (Euphorbia hirta L.) Herb Ethanol Extract. Asian Journal of Pharmaceutical Reseacrh and Development. 2021; 9(5):06-09.
29. Silva JMDS., Jackson R G D S A., Cristiane D S C A., Daniel A N., Livia M O D., Camila D S A., Larissa A R and Ana P D O. Antimicrobial Activity from Species Plectranthus amboinicus (Lour.) Sprengm A review. Europoan Journal of Medical Plants. 2020; 31(18):1-14.
30. Wadikar DD and Prakash EP. Coleus aromaticus: a therapeutic herb with multiple potentials. J Food Sci Technol. 2016; 53(7): 2895-2901.
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How to Cite
Singarimbun, N., Firmawati Zega, D., Firmawati Zega, D., Anjelina Simanjuntak, H., Purba, H., & Gurning, K. (2022). Phytochemicals of Extract N-Hexane Leaves Bangun-Bangun (Plectranthus Amboinicus) (Lour.) Spreng and Antibacterial Activity Causes Diarrhea. Asian Journal of Pharmaceutical Research and Development, 10(3), 17-20. https://doi.org/https://doi.org/10.22270/ajprd.v10i3.1133