Analysis of Herbal Drugs by HPTLC: A REVIEW

  • Gunjal Sanket B S.M.B.T. College of Pharmacy, Dhamangaon, Tal. Igatpuri, Dist. Nashik
  • Dighe P. R S.M.B.T. College of Pharmacy, Dhamangaon, Tal. Igatpuri, Dist. Nashik

Abstract

Densitometric evaluation through HPTLC is very useful method for standardization of medicinal plants and other natural products, especially those used in different system of medicine. It has been approved as an authenticated method of analysis in several pharmacopeias, including the USP and the IP. Result from a number of medicinal plants studied in the laboratory have shown that, TLC- densitometry is more advantageous strategy than HPLC or GLC. Improvement in resolution, HPTLC is valuable tool for the investigation of herbal products with respect to different aspects of their quality.  sensitivity and reproducibility are the fundamental attributes. HPTLC plays an important role in the characterization of marker compounds for the development and standardization of herbal medicines. The HPTLC approach is particularly well suited to the examination of herbs and herbal preparations because it allows for easy comparison of samples using fingerprints and quantitative determination using scanning densitometry. Herbs and herbal mixtures are particularly challenging to standardise. Many of the current medications we use today for various ailments are based on plants and plant-based products. This review focused on the various HPTLC methods used to analyse herbal drugs. For each drug, extraction processes as well as the HPTLC analytical method have been optimised.


 

Keywords: HPTLC, Densitometric evaluation, Medicinal plants, Herbal medicines, Quantitative determination.

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Author Biographies

Gunjal Sanket B, S.M.B.T. College of Pharmacy, Dhamangaon, Tal. Igatpuri, Dist. Nashik

S.M.B.T. College of Pharmacy, Dhamangaon, Tal. Igatpuri, Dist. Nashik

Dighe P. R, S.M.B.T. College of Pharmacy, Dhamangaon, Tal. Igatpuri, Dist. Nashik

S.M.B.T. College of Pharmacy, Dhamangaon, Tal. Igatpuri, Dist. Nashikvvvvv

References

1. Sethi PD. HPTLC Qualitative analysis of pharmaceutical formulations. CBS Publishers, First edition. 1996.
2. Shewiyo DH, Kaale E, Risha PG, Dejaegher B, Smeyers-V. J, Heyden Y. V. HPTLC methods to assay active ingredients in pharmaceutical formulations: A review of the method development and validation steps. Journal Pharmaceutical Biomedical Analysis. 2012; 66:11–23.
3. Singh H, Mishra A, Mishra AK. Isolation, characterization and quantification of a bioactive compound from Cleome viscosa L. seeds by HPTLC-Densitometric method. South African Journal Botany. 2018; 117:169–173.
4. Ahmed S, Al-Rehaily AJ, Alam P, Alqahtani AS, Hidayatullah S, Rehman MT. Antidiabetic, antioxidant, molecular docking and HPTLC analysis of miquelianin isolated from Euphorbia schimperi C. Presl. Saudi Pharmaceutical Journal. 2019; 27(5):655–63.
5. Deattu N, Suseela L, Narayanan N. Chromatographic analysis of polyherbal extract and formulation by HPTLC and GC–MS methods. Journal Pharmacy Research. 2013; 6(1):6–10.
6. Chewchinda S, Kongkiatpaiboon S. A validated HPTLC method for quantitative analysis of morin in Maclura cochinchinensis heartwood. Chinese Herbal Medicines. 2020;12(2):200–3.
7. Gupta A, Sheth NR, Pandey S, Shah DR, Yadav JS. Determination of ursolic acid in fractionated leaf extracts of Ocimum gratissimum Linn and in developed herbal hepatoprotective tablet by HPTLC. Pharmacognocy Journal . 2013; 5(4):156–62.
8. Kaur P, Gupta RC, Dey A, Kumar Pandey D. Simultaneous quantification of oleanolic acid, ursolic acid, betulinic acid and lupeol in different populations of five Swertia species by using HPTLC-densitometry: Comparison of different extraction methods and solvent selection. Industrial Crops and Products. 2019; 130:537–46.
9. Rouhani M. Modeling and optimization of ultrasound-assisted green extraction and rapid HPTLC analysis of stevioside from Stevia Rebaudiana. Industrial Crops and Production. 2019; 132:226–35.
10. Irshad S, Khatoon S. A validated HPTLC method for the simultaneous determination of seasonal alterations of two antihypertensive monoterpenoid indole alkaloids in Rauvolfia species from northern India. South African Journal of Botany. 2021; 142:193–200.
11. Deore SL, Mohod MA, Baviskar BA, Khadabadi SS. HPTLC validated stability indicating assay method for marketed herbal antihypertensive formulations. Pharmaceutical Methods. 2013; 4(1):11–5.
12. Gomathi D, Ravikumar G, Kalaiselvi M, Vidya B, Uma C. HPTLC fingerprinting analysis of Evolvulusalsinoides (L.) L. Journal of Acute Medicine. 2012; 2(3):77–82.
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How to Cite
B, G., & R, D. (2022). Analysis of Herbal Drugs by HPTLC: A REVIEW. Asian Journal of Pharmaceutical Research and Development, 10(2), 125-128. https://doi.org/https://doi.org/10.22270/ajprd.v10i2.1056