Evaluation of Antidiabetic Potential of Eucalyptus Globulus Plant Extract In Alloxan-Induced Diabetic Rats
DOI:
https://doi.org/10.22270/ajprd.v9i5.1021Keywords:
Antidiabetic potential, OGTT, Eucalyptus globulus, Alloxan, Insulin, Polyphenols.Abstract
Objective: Evaluation of Antidiabetic Potential of Eucalyptus globules Plant Extract in Alloxan- Induced Diabetic Rats.
Method: Methanolic leaves extract of Eucalyptus globulus plant was prepared by Soxhlet extraction method and stored in refrigerator at 4℃ for two days before use. Male Albino Wistar rats were made diabetic at the dose of Alloxan (150mg/kg/day i.p.). Methanolic leaves extract of Eucalyptus globulus plant (200mg/kg, 400mg/kg & 600mg/kg/day p.o.) was screened for antidiabetic activity. Standard drug Glibenclamide (0.5mg/kg/day i.p.) was administered to the second group of animals for 14 days. Blood glucose level and body weight of rats were recorded on Initial & Final days of treatment. Further hypoglycemic & OGTT evaluation were done. At the end of treatment biochemical estimations & histopathological examination of pancreas were also carried out.
Result: The statistical data indicated, 14 Days oral administration of Methanolic leaves extract of Eucalyptus globulus plant caused a significant (????< 0.05) reductions in blood glucose level, hypoglycemicpotential, significant oral glucose tolerance &gain in body weight as compared with toxic control group. Further showed improvement in altered biochemical parameters associated with diabetes. Concurrent histopathological examination of pancreas of these animals showed regeneration by Methanolic leaves extract which were earlier necrosed by Alloxan.
Conclusion: Results obtained in this study substantiate the Antidiabetic potential of Methanolic leaves extract of Eucalyptus globulus plant the source of Ellagitannins a bioactive polyphenol and could be considered for further evaluation in clinical studies and drug development.
Downloads
References
2. Jain, V., Viswanatha, G. L., Manohar, D., & Shivaprasad, H. N.Isolation of antidiabetic principle from fruit rinds of Punica granatum. Evidence-Based Complementary and Alternative Medicine, 2012. https://doi.org/10.1155/2012/147202
3. Antidiabetic Activity on the Extracts of Embelia ribes in Streptozotocin Induced Diabetic Rats _ Insight Medical Publishing. (n.d.).
4. Dholi, S. K., Raparla, R., Mankala, S. K., & Nagappan, K. Invivo Antidiabetic evaluation of Neem leaf extract in alloxan induced rats. 2011; 01(04), 100–105.
5. Vijayanand, S. Evaluation of Antidiabetic activity of Murraya koenigii on Alloxan Induced Diabetic rats. December.2016, 2015.
6. Ito, H. Metabolites of the Ellagitannin Geraniin and Their Antioxidant Activities. 2011; 1110–1115.
7. Somani R., Kasture S., Singhai A.K. Antidiabetic potential of Butea monosperma in rats. Fitoterapia. 2006;77:86–90.
8. Puri D. The insulinotropic activity of a Nepalese medicinal plant Biophytum sensitivum: preliminary experimental study. J Ethnopharmacol. 2001; 78(1):89–93.
9. Pari L., Maheswari J.U. Hypoglycemic effect of Musa sapientum L. in alloxan-induced diabetic rats. J. Ethnopharmacol. 1999; 68:321–325.
10. Chaudhary, S., Khosa, R. L., Jha, K. K., & Verma, N. Evaluation of Antidiabetic Activity of Cressa Cretica. 2010; 188, 181–188.
11. King, A. J. F.The use of animal models in diabetes research. https://doi.org/10.1111/j.2012;1476-5381,01911.x
12. Glugliano D., Ceriello A., Paolisso G. Oxidative stress and diabetic vascular complications. Diabet. Care. 1996; 19:257–267.
13. Rats, D.Evaluation of Antidiabetic Activity of Ethanolic Extract of Ajuga Parviflora in Diabetic Rats. 2019; 9(4), 112–115.
14. Mathew P.T., Augusti K.T. Hypoglycemic effects of onion, Allium cepa Linn. on diabetes mellitus- a preliminary report. Ind. J. Physiol. Pharmacol. 1975; 19:213–217.
Published
How to Cite
Issue
Section
AUTHORS WHO PUBLISH WITH THIS JOURNAL AGREE TO THE FOLLOWING TERMS:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).