Ameliorative Effect of Allium Cepa L. (Red Onion) Extract against Potassium Bromate Induced Intestinal Injury‏ In Rats

  • Nadia M. Kermani Department of Zoology, Faculty of Science, Zawia University. Zawia, Libya
  • Fikry A Abushofa Department of Zoology, Faculty of Science, Zawia University. Zawia, Libya
  • Amera M. Adaek Department of Zoology, Faculty of Science, Zawia University. Zawia, Libya
  • Fathia G. Jaat Department of Zoology, Faculty of Science, Zawia University. Zawia, Libya
  • Fathia G. Shakhtour Food Science and Technology Department, Faculty of Agriculture, University of Tripoli, Libya.

Abstract

Potassium bromate is an oxidizing agent and one of the cheapest dough improvers in the baking and food industry. This agent is classified as carcinogenic in rats and nephrotoxic in both man and experimental animals when given orally. The red onion, Allium cepa, is used in the daily diet for taste. Compounds in onions also have various medicinal properties, such as being anticancer, antifungal, antioxidant, anti-ulcer, and anti-inflammatory. We therefore sought to investigate whether aqueous extract of red onion would be protective against injury to the intestine


Materials and Methods: 36 Wistar male rats were divided into 6 batches. Over a 4-weeks experimental period, group I rats served as a control and group II received 100 mg/kg b.w of KBrO3 on the 24th and 27th days. Group III received red onion juice at 1ml/100 g bw every day and 100 mg/kg bw of KBrO3 on the 24th and 27th days. Group IV received 50 mg/kg bw of KBrO3 twice per week, group V received red onion juice daily at 1ml/100 g bw and 50 mg/kg bw of KBrO3 twice per week, and group VI received 30 mg/kg KBrO3 every day. The body and four organs weights, including the kidney, testis, lung and liver of all experimental rats were measured. The histopathological investigation was performed for the intestine tissues of all groups.


Results: KBrO3-treated small intestines exhibited destruction in the villi, decreased number of goblet cells, crypt loss and cell infiltration in the epithelial lining. Treatment with extract of red onion appeared to significantly ameliorate the toxic effects of KBrO3.


Conclusion: Our findings suggest that red onion ameliorates the extent of intestinal injury and appears to act as an antioxidant. This study supports that red onion has beneficial properties, although no direct clinical conclusions can be drawn from these data.


 

Keywords: Potassium bromate, histopathological, red onion, intestine, Wistar rats, protective

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Author Biographies

Nadia M. Kermani, Department of Zoology, Faculty of Science, Zawia University. Zawia, Libya

Department of Zoology, Faculty of Science, Zawia University. Zawia, Libya

Fikry A Abushofa, Department of Zoology, Faculty of Science, Zawia University. Zawia, Libya

Department of Zoology, Faculty of Science, Zawia University. Zawia, Libya

Amera M. Adaek, Department of Zoology, Faculty of Science, Zawia University. Zawia, Libya

Department of Zoology, Faculty of Science, Zawia University. Zawia, Libya

Fathia G. Jaat, Department of Zoology, Faculty of Science, Zawia University. Zawia, Libya

Department of Zoology, Faculty of Science, Zawia University. Zawia, Libya

Fathia G. Shakhtour, Food Science and Technology Department, Faculty of Agriculture, University of Tripoli, Libya.

Food Science and Technology Department, Faculty of Agriculture, University of Tripoli, Libya.

References

1. Branen AL, Davidson PM, Salminen S, Thorngate J. Food additives. New York: Taylor & Francis. 2001.
2. Chauhan D, Jain P. A. Scientific study of genotoxic-carcinogenic impacts of Potassium Bromate as food additive on human health. International Research Journal of Engineering and Technology. 2016 ;(3): 1136-1139
3. Ahmad MK, Naqshbandi A, Fareed M, Mahmood R. Oral administration of a nephrotoxic dose of potassium bromate, a food additive, alters renal redox and metabolic status and inhibits brush border membrane enzymes in rats, Food chemistry. 2012 ; (134): 980-985
4. Ahmad MK, Khan AA, Ali SN, Mahmood R. Chemoprotective effect of taurine on potassium bromate-induced DNA damage, dna-protein cross-linking and oxidative stress in rat intestine. Public Library of Science ONE. 2015; 10 (3).
5. Fawell J, Walker M. Approaches to determining regulatory values for carcinogens with particular reference to bromate. Toxicology. 2006; 221(2-3): 149-153.
6. Qin J, Kim MS, Chao K, Dhakal S, Cho BK. Non-targeted and targeted raman imaging detection of chemical contaminants in food powders, Proc. Sensing for Agriculture and Food Quality and Safety. 2018 X, 10665: 106650G
7. Khan N. Nigella .Sativa (black cumin) ameliorates potassium bromate-induced early events of carcinogenesis: diminution of oxidative stress. Human & Experimental Toxicology. 2003; (22):193-203.
8. Bao L, Yao XS, Tsi D, Yau CC, Chia CS, Nagai H, et al. Protective effects of bilberry (Vaccinium myrtillus L.) extract on KBrO3-induced kidney damage in mice. Journal of Agricultural and Food Chemistry. 2008; (56): 420-425
9. Ahmad MK, Khan AA, Mahmood R. Taurine ameliorates potassium bromate-induced kidney damage in rats. Public Library of Science ONE. 2013; 45 (5): 1109-21
10. Ali BH, Al Za’abi M, Karaca T, Al Suleimani Y, Al Balushi KA, et al. Potassium bromate-induced kidney damage in rats and the effect of gum acacia thereon. American Journal of Translational Research. 2018; (10): 126-137
11. Santas J, Almajano MP and Carbo R. Antimicrobial and antioxidant activity of crude onion (Allium cepa, L) extracts. International Journal of Food Science. 2010; (45): 403-409.
12. Rose P, Whiteman M, Moore PK, Zhu ZY. Bioactive S-alk(en)yl cysteine sulphoxide metabolites in the genus Allium: The chemistry of potential therapeutic agents. Natural Product Reports. 2005; (22): 351-368
13. Kermani NM, Abushofa FA, Aldaek AM, Jaat FG. Protective Effect of Allium cepa L.(onion) Against Potassium Bromate-Induced Hematological, Biochemical and Histopathological Alterations in Rats. International Journal of Innovative Science and Research Technology. 2020; 5(11):201-207
14. Dimkpa U, Ukoha U, Anyabolu E, Uchefuna R, Anikeh L, et al. Hepatotoxic Effects of Potassium Bromate on Adult Wistar Rats. Journal of Biology, Agriculture and Healthcare. 2013; 3(7): 111-115
15. Slaoui M, Fiette L. Histopathology procedures: from tissue sampling to histopathological evaluation. Methods in Molecular Biology. 2011; (91):69-82
16. Ige SF, Akhigbe RE, Edeogho O, Ajao FO, Owolabi OQ, et al. Hepatoprotective activities of Allium cepa in cadmium-treated rats. International Journal of Pharmacy and Pharmaceutical Sciences. 2011 ;( 3): 60-63.
17. Ahmed AF, Al-Yousef HM, Al-Qahtani JH, Al-Said MS. A hepatonephro-protective phenolic-rich extract from red onion (Allium cepa L.) peels. Pakistan Journal of Pharmaceutical Sciences. 2017; 30(5):1971-1979.
18. Ige SF, Salawu EO, Olaleye SB, Badmus J, Adeleke AA. Onion (Allium cepa) extract prevent cadmium induced renal dysfunction. Indian Journal of Nephrology. 2009; (19): 140-4.
19. Ara C, Asmatullah SH, Andleeb S, Zahid B, Arshad M. et al. Red Onion Extract Attenuates Aluminum Induced Toxicity in Swiss Albino Mice. Pakistan Journal of Zoology. 2020; 1-4.
20. Ola-Mudathir KF, Suru SM, Fafunso MA, Obioha UE, Faremi TY.. Protective roles of onion and garlic extracts on cadmium-induced changes in sperm characteristics and testicular oxidative damage in rats. Food and Chemical Toxicology. 2008; 46(12): 3604-3611.
21. Kurokawa Y, Maekawa A, Takahashi M, Hayashi, Y. Toxicity and carcinogenicity of potassium bromate - a new renal carcinogen. Environmental Health Perspectives. 1990; (87): 309-335
22. Isoda T, Nakatsu Y, Yamauchi K, Piao J, Yao T, Honda H, Nakabeppu Y, Tsuzuki T, Abnormality in Wnt signaling is causatively associated with oxidative stress-induced intestinal tumorigenesis in MUTYH-null mice. International Journal of Biological Sciences. 2014;10(8): 940
23. Piao J, Nakatsu Y, Ohno M, Taguchi K.I, Tsuzuki T, Mismatch repair deficient mice show susceptibility to oxidative stress-induced intestinal carcinogenesis. International journal of biological sciences. 2014; 10(1):73
24. Aoki Y, Taniguchi Y, Matsumoto M, Matsumoto M, Ohno M, et al. Oxidative-stress-driven in vivo mutagenesis induced by oral administration of potassium bromate in the small intestines of gpt delta mice. Mutation Research/ Genetic Toxicology and Environmental Mutagenesis. 2020; 503136
25. Circu ML, Aw TK. Redox biology of the intestine. Free Radical Research. 2011;(45): 1245-1266
26. Shahid F, Farooqui Z, Khan AA, Khan F. 2018. Oral Nigella sativa oil and thymoquinone administration ameliorates the effect of long-term cisplatin treatment on the enzymes of carbohydrate metabolism, brush border membrane, and antioxidant defense in rat intestine. Naunyn-Schmiedeberg's Archives of Pharmacology, 391: 145-157.
27. Shahid F, Farooqui Z, Rizwan S, Abidi S, Parwez I. et al. Oral administration of Nigella sativa oil ameliorates the effect of cisplatin on brush border membrane enzymes, carbohydrate metabolism and antioxidant system in rat intestine. Exp. Toxicologic Pathology. 2017 ;( 69): 299-306.
28. Rezq A. Potential protective and ameliorate effects of sesame oil and jojoba oil against potassium bromate (KBrO3)- induced oxidative stress in rats. Journal of Studies and Searches of Specific Education, Faculty of Specific Education, Zagazig University. 2017; 3 (1):58-61.
29. Wang Y, Zhao H, Liu J, Shao Y, Li J, et al. Copper and arsenic-induced oxidative stress and immune imbalance are associated with activation of heat shock proteins in chicken intestines. International Immunopharmacology. 2018;( 60): 64-75
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Kermani, N., Abushofa, F., Adaek, A., Jaat, F., & Shakhtour, F. (2021). Ameliorative Effect of Allium Cepa L. (Red Onion) Extract against Potassium Bromate Induced Intestinal Injury‏ In Rats. Asian Journal of Pharmaceutical Research and Development, 9(5), 1-5. https://doi.org/https://doi.org/10.22270/ajprd.v9i5.1012