Pork DNA Contamination in Pharmaceutical Products: A Review

  • Nonik Widya Firma School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147
  • Zikra Azizah School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147
  • Ridho Asra School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147


Objectives: A country with a muslim majority population, halal in food product as well as no food product including pharmaceutical product has been main concerned. Several materials which used on pharmaceutical product such as gelatin, collagen and fat content synthesized or extracted from mammalia such as bovine, fish, and also porcine. Product sourced from pork and its derivatives was prohibited for muslim . This review presents about methods to detect pork content in pharmaceutical products and information about pharmaceutical products that are positively contaminated with pork ingredients and their derivatives related to halal status for muslims.

Data Sources Study Selection: The method used in this research was study of literature. Literatures used in this paper were based on publication of the last ten years (2010-2020). Data sources of this review article taken from various online journal search sites such as digital library, Google, Google Scholar, PubMed, Science Direct, and E-Resources.

Conclusion: Conclusion: A result of this review were found 36 capsule samples, 7 gelatin of soft candy gelatin, 4 cosmetic product, amniotic fluid, placenta, oleic acid, stearate acid/stearic alcohol, lanolin alcohol and propylene glycol are positive contain pork DNA. The analytical method used to detect the content of pork and its derivatives in pharmaceutical products such as PCR, HPLC, Nano UPLCESI-Q-TOFMS, FTIR, Liquid Chromatography–Tandem  Mass Spectroscopy, Liquid Scintillation Counting, PEME, ELISA, and SDS-PAGE Both Conventional and Combined.


Keywords: Pork DNA, Pharmaceutical products, Cosmetic product, Detection method


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Author Biographies

Nonik Widya Firma, School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147

School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147

Zikra Azizah, School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147

School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147

Ridho Asra, School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147

School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147


1. Hashim P, & Mat Hashim D. A Review of Cosmetic and Personal Care Products: Halal Perspective and Detection of Ingredient. Pertanika J. Sci. Technol, 2013; 21(2):281-292.
2. Hassan N, Ahmad T, & Zain N.M. Chemical and Chemometric Methods for Halal Authentication of Gelatin: An Overview. J. Food Sci, 2018; 83(23): 2903–2911.
3. Hunter M. The Emerging Halal Cosmetic and Personal Care Market. Personal Care, 2012; 37–41.
4. Ismail Z, & Ehsan A.H. Halal Nutraceutical Market: Issues and Challenges. Segi Review, 2010; 3(2):96–117.
5. Aziz A.N, Ibrahim I, & Abdul Raof N. The Need for Legal Intervention Within The Halal Pharmaceutical Industry. Procedia-Social and Behavioral Science, 2012; 121:124–132.
6. Hanzaee K.H, & Ramezani M.R. Intention to Halal Products in The World Markets. Interdisciplinary Journal of Research in Business, 2011; 1(5): 1-7.
7. Mursyidi A. The Role of Chemical Analysis in The Halal Authentication of Food and Pharmaceutical Products. Journal of Food and Pharmaceutical Sciences, 2013, 1(1): 1-4.
8. Ballin N. Z. Authentication of Meat and Meat Products. Meat Sci, 2010; 86(3):577–87.
9. Raraswati M.A, Triyana K, & Rohman A. Differentiation of Bovine and Porcine Gelatins in Soft Candy Based on Amino Acid Profiles and Chemometrics. J Food Pharm Sci, 2013; 2(1):1–6.
10. Huang C.Y, Kuo J.M, Wu S.J, & Tsai H.T. Isolation and Characterization of Fish Scale Collagen from Tilapia (Oreochromis sp.) by A Novel Extrusion-Hydro-Extraction Process. Food Chemistry. 2016; 190:997–1006.
11. Hosen, & Nadirsyah. Hilal and Halal: How to Manage Islamic Pluralism in Indonesia?. Asian Journal of Comparative Law, 2012; 7(1):11-12.
12. Badan Pengawasan Obat dan Makanan Republik Indonesia. Pencantuman Informasi Asal Bahan Tertentu Kandungan Alkohol, dan Batas Kedaluwarsa pada Penandaan/Label Obat, Obat Tradisional, Suplemen Makanan, dan Pangan. Peraturan Kepala Badan Pengawas Obat dan Makanan Republik Indonesia Nomor HK. Jakarta: Badan Pengawasan Obat dan Makanan Republik Indonesia; 2010.p.4-5.
13. Sahilah Mohd A.M, Fadly L, Norrakiah A.S, Aminah A, Wan Aida W.M, Ma'aruf Mohd. A.G, & Khan A. Halal Market Surveillance of Soft and Hard Gel Capsules in Pharmaceutical Products Using PCR and Southern-Hybridization on The Biochip Analysis. Int Food Res J, 2012; 19(1):371–5.
14. Azira T, Che Man Y.B, Raja Mohd Hafidz R.N, Aina M.A, Amin I. Use of Principal Component Analysis for Differentiation of Gelatine Sources Based on Polypeptide Molecular Weights. Food Chemistry, 2014; 151:286–292.
15. Shabani H, Mehdizadeh M, Mousavi S.M, Dezfouli E.A, Solgi T, Khodaverdi M, Rabiei M, Rastegar H, & Alebouyeh M. Halal Authenticity of Gelatin Using Species-Specific PCR. Food Chemistry, 2015; 184:203–206.
16. Food and Drug Administration 2006. Alphabetical List of SCOGS Substances. Retrieved from www.cfsan.fda.gov/*dms/ opascogc 23 July 2020.
17. Rohman A, Che Man Y.B. Analysis of Pig Derivatives for Halal Authentication Studies. Food Rev Int, 2012; 28(1):97–112.
18. Yang H, & Shu Z. The Extraction of Collagen from Pig Skin. Journal of Chemical and Pharmaceutical Research, 2014; 6(2):683- 687.
19. Liu Q, Kong B, Xiong Y.L, & Xia X. Antioxidant Activity and Functional Properties of porcine Plasma Protein Hydrolysate As Influenced By The Degree Of Hydrolysis. Food Chemistry, 2010; 118(2):403–410.
20. Pati F, Adhikari B, & Dhara S. Isolation and Characterization of Fish Scale Collagen of Higher Thermal Stability. Bioresource Technology, 2010; 101(10):3737–3742.
21. Zhan J, Duan R, Ye C, & Konno K. Isolation and Characterization of Collagens from Scale of Silver Carp (Hypophthalmichys molitrix). Journal of Food Biochemistry, 2010; 34:1343–1354.
22. Hoyer B, Bernhardt A, Lode A, Heinemann S, Sewing J, Klinger M, Notbohm H, & Gelinsky M. Jelly Fish Collagen Scaffolds for Cartilage Tissue Engineering. Acta Biomaterialia, 2014; 10(2):883– 892..
23. Sugibayashi K, Yusuf E, Todo H, Dahlizar S, Sakdiset P, Arce F.J.R, & See G.L. Review Halal Cosmetics: A Review on Ingredients, Production, and Testing Methods. Cosmetics, 2019; 6(37):1-17.
24. Mohammad A.W, Suhimi N.M, Abdul Aziz A.G.K, & Jahim J.M. Process for Production of Hydrolysed Collagen from Agriculture Resources: Potential for Further Development. Journal of Applied Sciences, 2014; 14(12):1319-1323.
25. Manikas K, & Hansen K.M. Software Ecosystems-A Systematic Literature Review. Journal of Systems and Software, 2013; 86(5):1294–1306.
26. Nikzad J, Shahhosseini S, Tabarzad M, Varcheh N.N, & Thosabi M. Simultaneous Detection of Bovine and Porcine DNA in Pharmaceutical Gelatin Capsules by Duplex PCR Assay for Halal Authentication. DARU Journal of Pharmaceutical Sciences, 2017; 25(3):1-11.
27. Widayat W.T, Winarni Agustini M, Suzery A, Ni’matullah Al-Baarri S, Rahmi Putri, & Kurdianto K. Real Time-Polymerase Chain Reaction (RT-PCR) sebagai Alat Deteksi DNA Babi dalam Beberapa Produk Non-Pangan. Indonesia Journal of Halal, 2019; 2(1):26-33.
28. Azilawati M, Hashim D, Jamilah B, & Amin I. RP-HPLC Method Using 6-Aminoquinolyl-N Hydroxysuccinimidyl Carbamate Incorporated with Normalization Technique in Principal Component Analysis to Differentiate The Bovine, Porcine And Fish Gelatins. Food Chem, 2015; 172:368–376.
29. Pratiwi D, Fitriani N.E, Sudjadi, & Rohman A. Application of Real Time Polymerase Chain Reaction for Analysis of Porcine DNA in Gelatine-Containing Capsule Shell for Halal Authentication. International Food Research Journal, 2018; 25(4):1515-1519.
30. Sudjadi Wardani H.S, Sepminarti T, & Rohman A. Analysis of Porcine Gelatin DNA in A Commercial Capsule Shell Using RealTime Polymerase Chain Reaction for Halal Authentication. Int J Food Prop, 2016; 19(9):2127–34.
31. Maryam St, Sismindari Raharjo T.J, Sudjadi, & Rohman A. Analysis of Porcine Contamination in Dendeng Using Mitochondrial D-loop 686 and Cyt B Gene Primers by Real Time Polymerase Chain Reaction. International Journal of Food Properties, 2015; 19:187-195.
32. Mutalib S.A, Muin N.M, Abdullah A, Hassan O, Mustapha W.A.W, Sani N.A, & Maskat M.Y. Sensitivity of Polymerase Chain Reaction (PCR)-Southern Hybridization and Conventional PCR Analysis for Halal Authentication of Gelatin Capsules. LWT-Food Science and Technology, 2015; 63(1):714–719.
33. Dermihan Y, Ulca P, & Zenyuva H.Z. Detection of Porcine DNA in Gelatine and Gelatine-Containing Processed Food Products-Halal/Kosher Authentication. Meat Science, 2012; 90(3):686-689.
34. Yilmaz M.T, Kesmen Z, Baykal B, Sagdic O, Kulen O, Kacar O, & Baykal A.T. A Novel Method to Differentiate Bovine and Porcine Gelatins in Food Products: Nano UPLC-ESI-Q-TOF-MS E Based Data Independent Acquisition Technique to Detect Marker Peptides in Gelatin. Food Chem, 2013; 141(3):2450–2458.
35. Sultana S, Motalib Hossain M.A, Azlan A, Johan M.R, Chowdhury Z.Z, & Eaqub Ali M. TaqMan Probe Based Multiplex Quantitative PCR Assay for Determination of Bovine, Porcine and Fish DNA in Gelatin Admixture Food Products and Dietary Supplements. Food Chemistry, 2010; 325:1-8.
36. Ali M.E, Razzak M.A, Hamid S.B.A, Rahman M.M, Al Amin M, & Rashid N.R.A. Multiplex PCR Assay for The Detection of Five Meat Species Forbidden in Islamic Foods. Food Chem, 2015; 177:214–224.
37. Sepminarti T, Wardhani H.S, Sudjadi, & Rohman A. Real-Time Polymerase Chain Reaction for Halal Authentication of Gelatin in Soft Candy. Asian Journal of Biochemistry, 2016; 11(1):34-43.
38. Zilhadia K.F, Betha O.S, & Supandi. Differentiation of Bovine and Porcine Gelatin Extracted from Vitamin C Gummy by Combination Method of Fourier Transform Infrared (FTIR) and Principal Component Analysis (PCA). Pharmaceutical Sciences and Research (PSR), 2018; 5(2):90–96.
39. Fadhlurrahman, Wardani A.K, & Widyastuti E. Deteksi Gelatin Babi pada Soft Candy Menggunakan Metode PCR-RFLP Sebagai Salah Satu Pembuktian Kehalalan Pangan. Jurnal Teknologi Pangan, 2015; 16(2):81-88.
40. Sultana S, Hossain M.A.M, Naquiah N.N.A, & Ali Md.E. Novel Multiplex PCR-RFLP Assay Discriminates Bovine, Porcine and Fish Gelatin Substitution in Asain Pharmaceuticals Capsule Shells. Food Additives & Contaminants, 2018; Part A:1-12.
41. Zabidi A.R, Fauzi, F.N, Abd Razak F.N, Rosli D, Jamil M.Z.M., Wan Ibrahim W.K, & Yahaya N. Screening Porcine DNA in Collagen Cream Cosmetic Products. Food Research, 2019; 4(1):151 – 156.
42. Gani S.S.A, Mustafa S, Desa M.N.M, Mokhtar N.F.K, Hanapi U.K, Zakaria Z, Yahaya N, Sulaiman W.M.A, & Wan. Detection of Porcine Adulteration in Cosmetic Cream Formulation via TaqMan Probe Real-Time Polymerase Chain Reaction. International Journal of Engineering & Technology, 2018; 7(4.14):112-115.
43. Aviani N. Deteksi Cemaran Babi Pada Sediaan Kapsul Suplemen Kecantikan Di Kota Yogyakarta Dengan Metode PCR (Polymerase Chain Reaction). 2017; Http://ejournal.uajy.ac.id/12577.
44. Che Man Y.B, Mustafa S, Mokhtar N.F.K, Nordin R, & Sazili A.Q. Porcine-Specific Polymerase Chain Reaction Assay Based on Mitochondrial D-Loop Gene for Identification of Pork in Raw Meat. International Journal of Food Properties, 2012; 15(1):134-144.
45. Kim T, Kim S, Kang W.Y, Baek H, Jeon H.Y, Kim B.Y, Kim C.G, & Kim D. Porcine Amniotic Fluid As Possible Anti Wrinkle Cosmetic Agent. Korean J. Chem. Eng, 2011; 28(9):1839–1843.
46. Seretis A, & Tsiakaras P. Hydrogenolysis of Glycerol to Propylene Glycol by In Situ Produced Hydrogen from Aqueous Phase Reforming of Glycerol Over SiO2–Al2O3 Supported Nickel Catalyst. Fuel Process. Technol, 2016; 142:135–146.
47. Choi Y.L, Park E.J, Kim E, Na D.H, & Shin Y. Dermal Stability and In Vitro Skin Permeation of Collagen Pentapeptides (KTTS and Palmitoyl-KTTS). Biomol. Ther, 2014; 22(4):321–327.
48. Intarakumhaeng R,Wanasathop A, Li K. Effects of Solvents on Skin Absorption of Non Volatile Lipophilic and Polar Solutes Under finite Dose Conditions. Int. J. Pharm, 2018; 536(1):405–413.
49. Hashim D.M, Che Man Y.B, Norakhasa R, & Shuhaimi M. Potential Use of Fourier Transform Infrared Spectroscopy for Differentiation of Bovine and Porcine Gelatins. Food Chemistry, 2010; 118(3):856-860.
50. Hermanto S, & Fatimah W. Differentiation of Bovine and Porcine Gelatin Based on Spectroscopic and Electrophoretic Analysis. J. Food Pharma Sci, 2013; 1(3):68–73.
51. Rezazadeh M, Yamini Y, Seidi S, & Aghaei A. Pulsed Electromembrane Extraction for Analysis of Derivatized Amino Acids: A Powerful Technique for Determination of Animal Source of Gelatin Samples. Talanta, 2015; 136(1):190–197.
52. Tukiran N.A, Ismail A, Mustafa S. & Hamid M. Determination of Porcine Gelatin in Edible Bird’s Nest by Competitive Indirect ELISA Based on Anti-Peptide Polyclonal Antibody. Food Control, 2016; 59 :561–566.
53. Amin M.A, Ismail A, Nhari R, Hafidz R.M. & Che Man Y. Identification Polypeptide Biomarkers of Porcine Skin Gelatin by Twodimensional Electrophoresis. Food Res. Int. J, 2013; 20(3):1395–1399.
54. Malik A, Sutantyo M.L, Hapsari I, Sinurat A.V, Purwati E.M, Jufri M, & Suryadi H. Simultaneous Identification and Verification of Gelatin Type in Capsule Shells by Electrophoresis and Polymerase Chain Reaction. J. Pharma Investig, 2016; 46(5):1–11.
55. Nhari R.M.H.R, Ismail A, Man C, & Yaakob B. Analytical methods for gelatin differentiation from bovine and porcine origins and food products. J. food Sci, 2012; 77(1):42–46.
56. Nur Hanani Z.A, Roos Y.H, & Kerry J.P. Use of Beef, Pork and Fish Gelatin Sources in The Manufacture of Films and Assessment of Their Composition and Mechanical Properties. Food Hydrocolloid, 2012; 29(1):144-151.
57. Zilhadia Yahdiana H, Irwandi J, & Effionora A. Characterization and Functional Properties of Gelatin Extracted from Goat Skin. International Food Research Journal, 2018; 25(1): 275-281..
58. Liu D, Nikoo M, Boran G, Zhou P, & Regenstein J.M. Collagen and Gelatin. Annu Rev Food Sci Technol, 2015; 6(1):527–57.
59. Amqizal H.I.A, Al-Kahtani H.A, Ismail E.A, Hayat K, & Jaswir I. Identification and Verification of Porcine DNA in Commercial Gelatin and Gelatin Containing Processed Foods. Food Control, 2017; 78(1):297-303.
60. Lee J.H, Kim M.R, Jo C.H, Jung Y.K, Kwon K, & Kang T.S. Specific PCR Assays to Determine Bovine, Porcine, Fish and Plant Origin of Gelatin Capsules of Dietary Supplements. Food Chem, 2016; 211: 253-259.
61. Husieh M.K, Shih P.Y, Wei C.F, Vickroy T.W, Chou C.C. Detection of Undeclared Animal by-Products in Commercial Canine Canned Foods: Comparative Analyses by ELISA and PCR-RFLP Coupled with Slab Gel Electrophoresis or Capillary Gel Electrophoresis. J Sci Food Agric, 2016; 96(5):1659–1665.
62. Mane B.G, Mendiratta S.K, & Tiwari A.K. Beef Specific Polymerase Chain Reaction Assay for Authentication of Meat and Meat Products. Food Control, 2012; 28(2):246-249.
63. Ali M.A, Al Amin M, Hamid S.B.A, Hossain M.M, & Mustafa S. Lab-on-A-Chip-Based PCR-RFLP Assay for The Confirmed Detection of Short-Length Feline DNA in Food. Food Addit Contam, 2015; Part A(32):1373-1383.
64. Mohamad N.A, Mustafa S, El Sheikha A.F, Khairil Mokhtar N.F, Ismail A, & Ali M.E. Modification of Gelatin-DNA Interaction for Optimised DNA Extraction from Gelatin and Gelatin Capsule. J Sci Food Agric, 2016; 96(7):2344–51.
65. Yusuf Z.K. Polymerase Chain Reaction (PCR). Jurnal Saintek, 2010; 5(6):1-6.
66. Cai H, Gu X, Scanlan M.S, Ramatlapeng D.H, & Lively C.R. Real-time PCR Assays for Detection and Quantitation of Porcine and Bovine DNA in Gelatin Mixtures and Gelatin Capsules. Journal of Food Composition and Analysis, 2012; 25(1):83–87.
67. Zulfahmi Z. Deteksi Kontaminan Babi Pada Produk Makanan Menggunakan Teknologi DNA Molekuler, Kutubkhanah. J. Penelit. Sos. Keagamaan, 2015; 18(1):1-6.
68. Raharjo T.J, Cahyaningtyas W, Surajiman I, & Pranowo D. Validation of PCR-RFLP Testing Method to Detect Porcine Contamination in Chicken Nugget. Indonesian Journal of Chemistry, 2013; 12(3):302–307.
69. Nemati M, Oveisi M.R, Abdollahi H, & Sabzevari O. Differentiation of Bovine and Porcine Gelatins Using Principal Component Analysis. Journal of Pharmaceutical and Biomedical Analysis, 2014; 34(3):485–492.
70. Widyaninggar A, Triyana K, & Rohman A. Differentiation Between Porcine and Bovine Gelatin in Capsule Shells Based on Amino Acid Profiles and Principal Component Analysis. Indonesian Journal of Pharmacy, 2012; 23(2):104–109.
71. Kaur K, Osman S, & Maziha S. Predicting Working Women Purchasing Behaviour of Malaysian Halal Cosmetic Products by Using Theory of Planned Behaviour. International Academic Research Journal of Business and Management, 2014; 3(1):1-7.
72. Aoun I, & Tournois L. Building Holistic Brands: An Exploratory Study of Halal Cosmetics. Journal Of Islamic Marketing, 2015; 6(1):109-132.
73. Shafii Z, & Wan Siti Khadijah W.M.N. Halal Traceability Framework for Halal Food Production. World Appl. Sci. J, 2011; 17(Towards the Traceability of Halal and Thoyyiban Application):01-05.
74. Yorgancioglu A, & Bayramoglu E.E. Production of Cosmetic Purpose Collagen Containing Antimicrobial Emulsion with Certain Essential Oils. Industrial Crops and Products, 2013; 44:378-382.
75. Rodríguez M.I.A, Barroso L.G.R, & Sánchez M.L. Collagen: A review On Its Sources and Potential Cosmetic Applications. J. Cosmet. Dermatol, 2017; 17(1):20–26.
76. Briliana V, & Mursito N. Exploring Antecedents and Consequences of Indonesian Muslim youths Attitude Towards Halal Cosmetic Products: A Case Study in Jakarta. Asia Pac. Manag. Rev, 2017; 22(4):176–184.
77. Rohman A, & Salamah N. The Employment of Spectroscopic Techniques Coupled with Chemometrics for Authentication Analysis of Halal Pharmaceuticals. Journal of Applied Pharmaceutical Science, 2018; 8(10):63-68.
78. Waskitho D, Lukitalingsih, E, Sudjadi, & Rohman A. Analysis of Lard in Lipstick Formulation Using FTIR Spectroscopy and Multivariate Calibration: A Comparison of Three Extraction Methods. Journal of Oleo Science. 2016; 65(10):815-824.
79. Rohman Abdul, & Che Man Y.B. Analysis of Lard in Cream Cosmetics Formulations Using FTIR Spectroscopy and Chemometrics. Middle-East Journal of Scientific Research, 2011; 7(5):726-732.
80. Dole M.N, Patel P.A, Sawant S.D, & Shedpure P.S. Advance Applications of Fourier Transform Infrared Spectroscopy. International Journal of Pharmaceutical Sciences Review and Research, 2011; 7(2):159-166.
81. Kurniati E, Rohman A, & Triyana K. Analysis of Lard in Meatball Broth Using Fourier Transform Infrared Spectroscopy and Chemometrics. Meat Science, 2014; 96(1):94–98.
82. Lukitaningsih E, Sa’adah M, Purwanto, Rohman A. Quantitative Analysis of Lard in Cosmetic Lotion Formulationusing FTIR Spectroscopy and Partial Least Square Calibration. Journal of theAmerican Oil Chemists Society, 2012; 89(8):1537-1543.
83. Rohman A, Gupitasari I, Purwanto, Triyana K. Quantification of Lard in the Mixture with Olive Oil in Cream Cosmetics Based on FTIR Spectra and Chemometrics for Halal Authentication. Jurnal Teknologi (Sciences and Engineering), 2014; 69(1):113-119.
84. Kim Y.S, Yu H.K, Lee B.Z, & Hong K.W. Effect of DNA Extraction Methods on The Detection of Porcine Ingredients in Halal Cosmetics Using Real-Time PCR. Applied Biological Chemistry, 2018; 61(3):549-555.
85. Husni P, Putriana N.A, Wicaksono I.A. Metode Deteksi Kandungan Babi dan Alkohol dalam Eksipien Farmasi dan Produk Obat untuk Menjamin Kehalalan Sediaan Obat. Majalah Farmasetika, 2017; 2(1):1-7.
86. Zilhadia, Izzah A.N, & Betha O.S. Perbandingan Metode SYBR Green dan Hydrolysis Probe dalam Analisis DNA Gelatin Sapi dan Babi Menggunakan Real Time PCR. Jurnal Sains Farmasi & Klinis, 2017; 4(2):16-23.
87. Rahmawati, Sisimindari, Raharjo T.J, Sudjadi, & Rohman A. Analysis of Pork Contamination in Abon using Mitochondrial D-Loop 22 primer Using Real-Time Polymerase Chain Reaction Method. International Food Research Journal, 2016; 23(1): 370-374.
88. Balia R.L, Suryaningsih L, & Putranto W.S. Pengujian Pemalsuan Bakso dengan Daging Babi Melalui Pendekatan Ensimatis dan Molekuler pada UKM di Kawasan Pendidikan Jatinangor Kabupaten Sumedang. Dharmakarya : Jurnal Aplikasi Ipteks untuk Masyarakat, 2014; 3(2):70-72.
89. Rohman A, Himawati A, Triyana K, Sismindari, & Fatimah S. Identification of Pork in Beef Meatballs Using Fourier Transform Infrared Spectrophotometry and Real-time Polymerase Chain Reaction. International Journal of Food Properties, 2017; 20(3):654-661.
90. Wahyuni S, Maryam S, & Aminah. Validasi Metode Analisis Cemaran DNA Babi pada Bakso Sapi Menggunakan Primer Mitokondria D-Loop22 dengan Metode Polymerase Chain Reaction (PCR). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy), 2019; 5 (1):65-72.
91. Raharjo T.J, Alfiraza E.N, Enjelina E, & Pranowo D. Validation of A Non-Specific Dye Real-Time PCR Assay for Porcine Adulteration in Meatball Using ND5 Primer. Indones. J. Chem, 2017; 17(2):167–174.
92. Nooratiny, Sahilah A.M, Alfie A.R.A, & Farouk M.Y.M. DNA Extraction from Ghee and Beef Species Identification Using Polymerase Chain Reaction (PCR) Assay. International Food Research Journal, 2013; 20(5):2959-2961.
93. Erwanto Y, Sugiyono, Rohman A, Abidin M.Z, & Ariyani D. Identifikasi Daging Babi Menggunakan Metode PCR-RFLP Gen Cytochrome B dan PCR Primer Spesifik Gen Amelogenin. Jurnal Agritech, 2012; 32(4):370-377.
94. Erwanto Y, Sugiyono, Rohman A, Abidin M.Z, & Muslim E.Y.P. Identification of Pork Contaminant in Meat-balls of Indonesia Local Market Using Polymerase Chain Reaction-Restric-tion Fragment Length Polymorphism (PCR-RFLP). Journal of Asian Australas. J. Anim, Sci, 2014; 27(10):1487-1492.
95. Cheng X.L, Wei F, Xiao X.Y, Zhao Y.Y, Shi Y, Liu W, Zhang P, Ma S.C, Tian S.S, Lin, R.C. Identification of Five Gelatins by Ultra Performance Liquid Chromatography/Time-Of-Flight Mass Spectrometry (UPLC/Q-TOF-MS) Using Principal Component Analysis. J. Pharm. Biomed. Anal, 2012; 62:191–195.
96. Sha X.M, Zhang L.J, Tu Z.C, Zhang L.Z, Hu Z.Z, Li Z, Li X, Huang T, Wang H, Zhang L. The Identification of Three Mammalian Gelatins by Liquid Chromatography-High Resolution Mass Spectrometry. LWT Food Sci. Technol, 2018; 89:74–86.
97. Nurjuliana M, Che Man, & Mat Hashim. Rapid Identification of Pork for Halal Authentication Using The Electronic Nose and Gas Chromatography Mass Spectrometer with Headspace Analyzer. Meat Science, 2011; 88(4):638-644.
98. Azira N,T, Amin I, Che Man Y.B. Differentiation of Bovine and Porcine Gelatins in Processed Products Via Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Principal Component Analysis (PCA) Techniques. Int. Food Res. J, 2012; 19(3):1175–1180.
99. Bosque-Sendra J.M, Rodríguez L.C, Samblás C.R, & De la Mata A.P. Combining Chromatography and Chemometrics for The Characterization and Authentication of Fats and Oils from Triacylglycerol Compositional Data-A Review. Analytica Chimica Acta, 2012; 724:1-11.
100. Hajipour B, Gharache M, Hamidizade M.R, Mohammadian F. Raising Halal Cosmetic Awareness Among The Respective Consumers. International Journal of Academic Research in Business and Social Sciences, 2015; 5(7):338-349.
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How to Cite
Widya Firma, N., Azizah, Z., & Asra, R. ( ). Pork DNA Contamination in Pharmaceutical Products: A Review. Asian Journal of Pharmaceutical Research and Development, 8(5), 96-104. https://doi.org/https://doi.org/10.22270/ajprd.v8i5.845