Assess the Anti-Inflammatory and Analgesic Activity of Leaves of Raphanus Sativus – An In Vivo Design

  • Devi Manisha School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India
  • Karnati Naga Rashi School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India
  • Kapilavai Harini School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India
  • Narender Boggula School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India
  • Vasudha Bakshi School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India.
  • Mohammed Sayeed School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India

Abstract

Background: Natural products found from plants have been conveying a vital role among human being since ancient times. An enormous number of scientific reports evidenced of using the medicinal plants as natural remedies. Nowadays, the medicinal plant is using as an alternative medicine to synthetic drugs. The use of medicinal plants singly or in combination in treating different ailments has been practiced by traditional medical healers for a very long time. People of Mesoamerica have utilized plants and products from plants in curing and relieving ailments for centuries.


Aim: The present aim of the study is to assess the anti-inflammatory and analgesic activity of Ethanolic Extract of leaves of Raphanus sativus (EERS) on animal models and therefore to determine the scientific basis for its use in traditional medicine in the management of anti-inflammatory condition.


Methods: Anti-inflammatory activity was evaluated using carrageenan and histamine-induced paw edema methods. Analgesic activity of Ethanolic Extract of leaves of Raphanus sativus (EERS) was estimated against a hot plate, acetic acid induced writhing and formalin tests.


Results: The extracts showed significant anti-inflammatory and analgesic activities with a dose-dependent manner. Anti-inflammatory activity of olive tree extract at 250 and 500mg/kg doses was more important compared to the used standard drugs (p<0.05), in both carrageenan and histamine-induced paw edema tests. In analgesic assays, results showed that 500mg/kg dose of olive tree extract has a significant analgesic effect through both peripheral and central mechanisms.


Conclusion: This study provided evidence on the traditionally claimed uses of the plant in pain and inflammatory diseases, and Raphanus sativus could be potential source for development of new analgesic and anti-inflammatory drugs.


 

Keywords: Raphanus sativus, anti-inflammatory activity, Carrageenan-induced rat paw oedema, Hot plate test, writhing test.

Downloads

Download data is not yet available.

Author Biographies

Devi Manisha, School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India

School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India

Karnati Naga Rashi, School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India

School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India

Kapilavai Harini, School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India

School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India

Narender Boggula, School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India

School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India

Vasudha Bakshi, School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India.

School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India.

Mohammed Sayeed, School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India

School of Pharmacy, Anurag University, Venkatapur, Ghatkesar, Telangana, India

References

1. Ferrucci L, Corsi A, Lauretani F, Bandinelli S, Bartali B, Taub DD et. al. The origins of the age-related proinflammatory state. Blood. 2005; 105:2294-2299.
2. Narender Boggula, Ananda Kumar Chettupalli, Swetha Reddy Naram reddy, Vasudha Bakshi. Anti-diabetic effect of Alstonia scholaris linn bark in Alloxan induced diabetic rats. Journal of Global Trends in Pharmaceutical Sciences. 2017; 8(1):3590-3598.
3. Emmanuel S, Ignacimuthu S, Perumalsamy R and Amalraj T. Anti-inflammatory activity of Solanum trilobatum. Fitoterapia. 2006; 77:611-612.
4. Ch. Krishna Mohan, V. Soundarya, R. Vasanth Kumar, L. Kiran Kumar, K. Vamshi Sharathnath, B. Narender. In-vitro anti inflammatory activity of dried leaves of Borassus flabellifer. Indo American Journal of Pharmaceutical Sciences. 2016; 3(8):809-813.
5. Fito M, Cladellas M, De la Torre R, Marti J, Munoz D, Schroder H et. al. Anti-inflammatory effect of virgin olive oil in stable coronary disease patients: a randomized, crossover, controlled trial. Eur J Clin Nutr. 2008; 62:570-574.
6. Singh P, Singh J. Medicinal and therapeutic utilities of Raphanus sativus. International journal of plant, animal and environmental sciences. 2013; 3(2):103-105.
7. Rosa Martha Perez Gutierrez, Rosalinda Lule Perez. Raphanus sativus (Radish): Their Chemistry and Biology. The Scientific World Journal. 2004; 4:811-837.
8. Guisti MM, Ghanadan H, Wroslstad RE. Elucidation of the structure and conformation of red radish (Raphanus sativus) anthocyanins using one-and two dimensional nuclear magnetic resonance techniques. J Agric Food Chem. 1998; 46:4858-4863.
9. Shyamala, G, Singh PN. An analysis of chemical constituents of Raphanus sativus. Proc. Natl. Acad. Sci. India Sect. B. 1987; 57:157-159.
10. Ahmad F, Hasan I, Chishti D K, Ahmad H. Antibacterial Activity of Raphanus sativus Linn. Seed Extract. Global Journal of Medical Research. 2012; 12(11):25-34.
11. Sreelekshmi G, Pratibha K. A safe body through moolaka-Raphanus sativus linn. International Ayurvedic Medical Journal. 2015; 3(10):3091-3096.
12. Teklic T, Hancock J T, Engler M, Paradikovic N, Cesar V, Lepedus H, Stolfa I, Beslo D. Antioxidative responses in radish (Raphanus sativus l.) Plants stressed by copper and lead in nutrient solution and soil. Acta Biologica Cracoviensia Series Botanica. 2008; 50(2):79-86.
13. Alqasoumi S, AL-Yahya M, AL-Howiriny T, Rafatullah S. Gastroprotective effect of radish Raphanus sativus L. on experimental gastric ulcer models in rats. Farmacia. 2008; 56(2):204-214.
14. Badar A, Jan M. Effect of crude extract of Raphanus sativus roots on isolated trachea of albino rat. Pak j physiol. 2012; 8(1):23-26.
15. Shukla S, Chatterji S, Yadav DK, Watal G. Antimicrobial efficacy of Raphanus sativus root juice. International Journal of Pharmacy and Pharmaceutical Sciences. 2011; 3(5):89-92.
16. Rahman I, Biswas SK, Kirkham PA. Regulation of inflammation and redox signaling by dietary polyphenols. Biochem Pharmacol. 2006; 72:1439-1452.
17. Yaso Deepika Mamidisetti, Nikhila Yammada, Harihara Kumar Siddamsetty, Vasudha Bakshi, Narender Boggula. Phytochemical and analgesic, anti-inflammatory screening of methanolic extract of Ficus religiosa fruits – An in vivo design. The Pharma Innovation. 2018; 7(6):69-74.
18. N.K. Manaswini, S Nazneen, Garige Baba Shankar Rao, Narender Boggula, Vasudha Bakshi, Manda Ram Mohan Evaluation of Ocimum tenuiflorum and Syzygium aromaticum phenolic ethereal oils for In-vitro anti-inflammatory and anti-bacterial activities. Journal of Drug Delivery & Therapeutics. 2019; 9(2):93-96.
19. Bamgbose SOA, Noamesi BK. Studies on cryptolepine. Inhibition of carrageenan-induced edema by cryptolepine. Planta Med. 1981; 42:392-396.
20. Farshid A, Tamaddonfard E, Morvaridi A. Effects of histidine and dexamethasone on the local inflammation induced by histamine in rats. Vet Res Forum. 2011; 2:31-36.
21. Sutar NG, Pal SC. Evaluation of analgesic activity of leaf extracts of pergularia daemia [forsk] in experimental animals. Int J Pharm Pharm Sci. 2014; 6:137-139.
22. Omisore NOA, Adewunmi CO, Iwalewa EO, Ngadjui BT, Watchueng J, Abegaz BM, et. al. Antiniociceptive and anti-inflammatory effects of Dorstenia barteri (Moraceae) leaf and twig extracts in mice. J Ethnopharmacol. 2004; 95:7-12.
23. Dahiya RS, Kaur P, Kashyap P, Katoch N, Gupta S. Pharmacological evaluation of hydroalcohol and chloroform extracts of nyctanthes arbour-tristis l. for antioxidant, anti-inflammatory and analgesic activity. Int J Pharm Pharm Sci. 2014; 6:460-465.
24. Koster R, Anderson M, De Beer E. Acetic acid for analgesic screening. Fed Proc. 1959; 18:412.
25. Mahgoub AA. Grapefruit juice potentiates the anti-inflammatory effects of diclofenac on the carrageenan-induced Rat's Paw oedema. Pharmacol Res. 2002; 45:1-4.
26. Hunskaar S, Hole K. The formalin test in mice: dissociation between inflammatory and non-inflammatory pain. Pain. 1987; 30:103-114.
27. Raman R, Parthiban S, Karthikeyan S, Muthuraman MS, Sivasubramanian A. Antimicrobial and anti-inflammatory studies on sargassum wightii extracts. Int J Pharm Pharm Sci. 2014; 6:611-614.
28. Ribeiro IA, Rocha J, Sepodes B, Mota-Filipe H, Ribeiro MH. Effect of naringin enzymatic hydrolysis towards naringenin on the anti-inflammatory activity of both compounds. J Mol Catal B: Enzym. 2008; 52:8-13.
29. Vineger R, Truax JF, Selph JL. Quantitative studies of the pathway to acute carrageenan inflammation. Fed Proc. 1976; 35:2447-2456.
30. Di Rosa M, Giroud JP, Willoughby DA. Studies of the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine. J Pathol. 1971; 104:15-29.
31. Vasudevan M, Gunman KK, Parle M. Antinociceptive and anti-inflammatory effects of Thespesia populnea bark extract. J Ethnopharmacol. 2007; 109:264-270.
32. Linardi A, Costa SKP, DeSilva GR, Antunes E. Involvement of kinins, mast cells, and sensory neurons in the plasma exudation and paw edema induced by staphylococcal entrotoxin B in the mouse. Eur J Pharmacol. 2002; 399:235-242.
Statistics
20 Views | 20 Downloads
How to Cite
Manisha, D., Rashi, K., Harini, K., Boggula, N., Bakshi, V., & Sayeed, M. (2020). Assess the Anti-Inflammatory and Analgesic Activity of Leaves of Raphanus Sativus – An In Vivo Design. Asian Journal of Pharmaceutical Research and Development, 8(5), 44-51. https://doi.org/https://doi.org/10.22270/ajprd.v8i5.840

Most read articles by the same author(s)