The Degradation Rates of Natural Dyes from Natural Resources: A Review

  • Yesi Armalina School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147
  • Zikra Azizah School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147
  • Ridho Asra School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147


Objectives: Natural plants can produce natural dyes. These dyes are widely used in the pharmaceutical and food fields, such as betacyanin, anthocyanin, carotenoids, flavonoids and others. Several external factors could cause natural dyes to show instability or degradation, some of the external factors are temperature, pH, storage time and so forth. Research on various kinds of natural dyes shows the degradation of these natural dyes. Degradation can cause damage to these plants dyes, therefore steps are needed to prevent the degradation of these dyes. The measurement of degradation was carried out to prevent the deterioration of natural dyes from natural materials that will be used or consumed. The purpose of this review is to determine the rate of degradation of natural dyes sourced from nature in accordance with the results of the studies that have been conducted.

Data Sources Study Selection: Data sources of this review article taken from web Google Scholar, Science Direct, National Center for Biotechnology Information (NCBI), Scopus, and Pubmed.

Conclusion: Natural dyes can be used for manufacture of food or drinks, natural dyes from plants instability due to degradation. Degradation occur because temperature, pH, storage time and sunlight.


Keywords: Activation energy, Degradation, Dyes, Half-life, Natural substances, pH, Reaction order, Storage time,  Temperature.


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Author Biographies

Yesi Armalina, School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147

School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147

Zikra Azizah, School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147

School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147

Ridho Asra, School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147

School of Pharmaceutical Science (STIFARM) Padang, Indonesia 25147


1. Yusuf M, Shabbir M, & Mohammad F. Natural colorants: historical, processing and sustainable prospects, Nat Prod Bioprospect. 2017; 7:123-145.
2. Rymbai H, Sharma RR, & Srivastav M. Biocolorants and its implication in health anf food industry- a review. International Journal of PharmaTech Research, 2011; 3(4).
3. Sigurdson GT, Tang P, & Giusti MM. Natural colorants: food colorants from natural sources. Annual Revies of Food Science and Technology, 2017; 8:261-280.
4. Setiawan MAW, Nugroho EK., & Lestario LN. Ekstraksi betasianin dari kulit umbi bit (Beta vulagaris) sebagai pewarna alami. Jurnal Ilmu Pertanian, 2015; 27(1):38-43
5. Grzybowski A, & Majewski KK. What is color and how it is perceived? Clinic in Dermatology,2019; 37(5):392-401
6. Das M, Sacid A, Hossain, MDF, Jiang GH, Eun JB, & Ahmed M. Influence of extraction parameters and stability of betacyanins extracted from amaranth during storage. Journal Food Science and Technology, 2018; 56(2):643-653.
7. Visalakshi M, & Jawaharlal M. Healty hues-status and implication in industries- brief review. Journal of Agriculture and Allied Sciences, 2013; 3(2):42-51.
8. Maoka T. Carotenoids as natural functional pigments. Journal of Natural Medicines, 2019;7:1-16
9. Chew YM, Hung CH, & King VAE. Accelerated storage test of betalains extracted from peel of pitaya (Hylocereus cacti) fruit. Journal Food Science and Technology, 2019; 56:1595-1600.
10. Rahimi P, Abedimanesh A, Namin SAM, & Ostadrahimi A Betalains, the nature-inspired pigment, in health and diseases. Critical Reviews in Food Science and Nutrition, 2018; 59(1).
11. Jiang T, Mao Y, Sui L, Yang N, Li S, Zhenzhou Z, Wang C, Yin S, He J, & He Y. Degradation of anthocyanins and polymeric color formation during heat treatment of purple sweet potato extract at different pH. Food Chemistry, 2019; 274:460-470.
12. Akogou FUG, Kayode APP, Besten HMW, Linnermann AR, & Fogliano, V. Effects of processing and stroage on the stability of the red biocolorantsapigenidium from sorghum. LWT- Food Science and Technology, 2018; 90: 592-597.
13. Remini H, Mertz C, Belbahi A, Achir N, Dornire M, & Madani K. Degradation kinetic modelling of ascorbic acid and colour intensity in pasteurised blood orange juice durig storage. Food Chemistry, 2015; 173: 665-673.
14. Fenger JA, Moloney M, Robbins RJ, Collins TM, & Dangles O. The influence of acylation, metal binding and natural antioxidants on the thermal stability of red cabbage anthocyanins in neutral solutions. Food & Function, 2019; 10(10):6470-6751.
15. Lopez JAF, Angosto JM, Gimenez PJ, & Leon G. Thermal stability of selected red exctrats used as food colorants. Plant for Human Nutrition, 2013; 68:11-17.
16. Gimenez PJ, Lopez JAF, Angosto JM, & Obon JM., &Obon JM. Comparative thermal degradation patterns of natural yellow colorants used in foods. Plant Food for Human Nutrition, 2015; 70:380-387.
17. Argyropoulos D, & Muller J. Kinetics of change in colour and rosmarinic acid equivalents during concentive drying of lemon balm (Melissa officinalis L.). Journal of Applied Research on Medicinal and Aromatic Plants, 2014; 1(1):e15-e22.
18. Cisse M, Vailant F, Kane A, Nadiaya O, & Dornier M. Impact of the extraction procedure on the kinetics of anthocyanin and colour degradation of roselle extract during storage. Journal Science Food and Agriculture, 2011; 94: 1214-1221.
19. Guneser O. Pigment and color stability of beetroot betalains in cow milk during thermal treatment. Food Chemistry, 2016; 196: 220-277.
20. Pragalyaashree MM, Tiroutchelvame D, & Sashikumar S. Degradation kinetics of antocyanins extracted from roselle calyces (Hibiscus sabdariffa). Journal of Applied Pharmaceutical Science. 2018; 8(11):057-063.
21. Jaiswal AK, Gupta S, & Ghannam NA. Kinetic evaluation of colour, texture, polyphenols and antioxidant capacity of irishyork cabbage after blanching treatment. Food Chemistry, 2012; 131: 63-72.
22. Jaiswal AK., & Ghannam NA. Degradation kinetic modelling of color, texture, polyphenol and antioxidant capacity of york cabbage after microwave processing. Food Research International, 2013; 53:125-133.
23. Wibowo S, Grauwet T, Santiago JS, Tomic J, Vervoort L, Hendrikx M, & Loey AV. Quality changes of pasteurised orange juice during storage: a kinetic study of specific parameters and their relation to colour instability. Food Chemistry, 2015; 187: 140-151.
24. Idham Z, Muhammad II, & Sarmidi MR. Degradation kinetics and color stability of spray-dried encapsulated anthocyanins from Hibiscus sabdariffa L. Journal of Food Engineering, 2011; 35:522-542.
25. Cisilya T, Lestario LN, & Cahyanti MN. Kinetika degradasi serbuk antosianin daun miana (Coleousscutellario des L. Benth) Var. Crispa hasil mikroenkapsulasi. Chimica et Natura Acta, 2017; 5(3):146-152.
26. Patras A, Brunton NP, Donnell CO, & Tiwari BK. Effet of thermal processing on anthocyanin stability in foods; mechanism and kinetic degradation. Food Science & Technology, 2010; 21: 3-11.
27. Chandran J, Nishan P, Rekha SS, & Pandit AB. Degradation of colour in beetroot (Beta Vulgaris L.): a kinetic study. Journal Food Science and Technology, 2012; 51(10):2678-2684.
28. Prada AB, & Otero L. Effect of hyperbaric storage at room temperature on color degradation of strawberry juice. Journal of Food Engineering, 2016;109:141-143.
29. Santos CDd, Ismail M, Cassini AS, Marezak LDF, Tessaro IC, & Farid M. Effect of thermal and high pressure processing on stability of extracted from red beet stalks. Journal Food Science and Technology, 2017; 55(2):569-577.
30. Yusmita L, Kasim A, & Nurdi, H. Pengaruh suhu ekstraksi dan konsentrasi asam sitrat terhadap pigmen betasianin daun kremah merah (Alternata dentata) dan aplikasinya pada pangan. Pro Food (Jurnal Ilmu dan Teknologi Pangan), 2017; 3(1).
31. Patras A. Stability and colour evaluation of red cabbage waste hydroethanolic extract in presence of different food additivies or ingredients. Food Chemistry, 2018; 275:539-548.
32. Woo KK, Ngou FHN, Ago LS, Soong WK, & Tang PY. Stability of betalain pigment from red dragon fruit (Hylocereuspolyrhizus). American Journal of Food Technology, 2011;6(2):140-148.
33. Sari Y. Pengaruh pemanasan terhadap kestabilan pigmen betalain dari buah naga merah (Hylocereuspolyrhizus). Jurnal Pendidikan Kimia, 2018; 2(1).
34. Hidayat T, Pratjojo W, & Widiarti N. Uji stabilitas pigmen dan antioksidan ekstrak zat warna alami kulit buah naga. Indonesian Journal of Chemical Science, 2014; 3(2).
35. Khuzaimah S. Uji stabilitas pigmen hasil ekstraksi zat warna alami dari kulit buah naga (Hylocereusundatus). JTI, 2018; 2(2).
36. Kwartinigsih E, Prastika AK, & Triana DL. Ekstraksi dan uji stabilitas antosianin dari kulit buah naga merah super (Hylocereuscostaricensis). Prosiding, 2016
37. Sarkis JR, Jaeschke DP, Mercali GD, Tessaro LC, & Marczak LDF. Degradation kinetic of anthocyanins in blackberry pulp during ohmic and conventional heating. International Food Reasearch Journal, 2019; 26(1):87-97.
38. Sipahli S, Mohanlall V, & Mellem JJ. Stability and degradation kinetic of crude antocyanins extracts from H. sabdariffa. Food Science and Technology, 2017; 37(2):209-215.
39. Wu HY, Yang KM, & Chiang PY. Roselle anthocyanins: antioxidant porperties and stability to heat and pH. Molecules, 2018; 23(1357).
40. Fendri STJ, Martinus BA, & Haryanti MD. Pengaruh pH dan suhu terhadapstabilitas antosianin dari ekstrak kulit ubi jalar ungu (Ipomoea Batatas (L.) Lam). Chempublish Journal, 2018; 2(2).
41. Jie L, Xiao-ding L, Yun Z, Zheng-dong Z, Zhi-ya Q, Meng L, Shao-hua Z, Liu S, Meng W, & Lu Q. Identification and thermal stability of purple-fleshed sweet potato anthocyanins in aqueous solutions with various pH values and fruit juices. Food Chemistry, 2013; 136:1429-1434.
42. Mulyawanti I, Budijanto S, &Yasni S. Stability of anthocyanins during processing, storage and stimulated digestion of purple sweet potato pasta. Indonesian Journal of Agricultural Science, 2018; 19(1):1-8.
43. Herrero JAH, & Frutos MJ. Degradation kinetics of pigment, colour and stability of the antioxidant capacity in juice model system from six anthocyanin sources. International Journal of Food Science and Technology, 2011; 46:2550-2557.
44. Vital DL, Li Q, West L, & Meija EGd. Anthocyanin condensed forms do not affect color or chemical stability of purple corn pericarp extract stored under different pH. Food Chemistry, 2017; 232:639-647.
45. Goncalves EM, Abreu M, Brandio TSR, & Silva CLM. Degradation kinetics of colour, vitamin C and drip loss in frozen brocolli (Brassica oleracea L. ssp. Italica) during storage at isothermal and non-isothermal conditions. International Journal of Refrigeration, 2011; 34:2136-2144.
46. Musche BM, Speers RA, & Ruspasinghe HRV. Storage temperature impacts on anthocyanina degradation, color changes dan haze development in juice of “Merlot” and “Ruby” grapes (Vitis vinifera). Frontier in nutrition, 2012;5(100).
47. Moldova B, David L, Chishora C, & Cimpoiu C. Degradation kinetics of anthocyanins ferom European Cranberrybush (Viburnum opulus L.) fruit extracts, effests of temperature, pH and storage. Mocules, 2012; 17:11655-11666.
48. Bastante MJC, Hurtando N, Delgado A, & Heredia FJ. Impact of pH and temperature on the colour and betalain content of colombian yellow pitaya peel (Selenicereus Megalanthus). Journal Food Science and Technology, 2016; 53(5):2405-2413.
49. Ali MA, Yusof YA, Chin NI, Ibrahim MN, & Basra SMA. Drying kinetics and colour analysis of Moringa Oleifera leaves. Agriculture and Agricultural Science Procedia, 2014; 2:394-400.
50. Chunthawon S, Achariyaviriya S, Achariyaviriya A, & Nmasanguam K. Color kinetics of longan flesh drying at high temperature. Procedia Engineering, 2012; 32:104-111.
51. Mercali GD, Schwartz S, Marezak LDF, Tessaro IC, & Sastry S. Effect of the electric field frequency on ascorbic acid degradation during thermal treatment by ohmic heating. Journal Agric Food Journal Agric Food Chem, 2013; 65(25):5865-5870.
52. Sonawane A, Pathak S, & Pradhan RC. Effect of processing temperature on dynamic rheological properties and color degradation kinetics of bael fruit pulp. Journal on the Science of Food and Agriculture, 2020.
53. Ahmed J, Al-Salman F, & Almusallam AS. Effect of blanching on thermal color degradation kinetics and rheological behavior of rocket (Eruca sativa) puree. Journal of Food Engineering, 2013; 119:660-669.
54. Wawire M, Oey I, Mathooko FM, Njoroge CK, Shitanda D, & Hendrickx M. Kinetic of thermal inactivation of peroxidase and color degradation of African Cowpea (Vigna unguiculata) leaves. Journal of Food Science, 2016; 91(1).
55. Fratianni A, Albanese D, Mignogna R, Luciana C, Panfili G, & Matten MD Degradation of carotenoids in Apricot (Prunus armeniaca L.) during drying process. Plant Foods for Human Nutrition, 2013; 68(3):241-246.
56. Kara S, & Ercelebi EA. (2013). Thermal degradation kinetics of anthocyanins and visual colour of Ummu mulberry (Morus nigra L.). Journal of Food Engineering, 2013; 116:541-547.
57. Lixia H, Ling B, & Shaojin W. Kinetic of color degradation of chesnut kernel during thermal treatment and storage. International Journal Agriculture & Biological Eng, 2015; 8(4).
58. Rengku PM, Ridhay A, & Prismawiryanti. Ekstraksi dan uji stabilitas betasianin dalam ekstrak buah kaktus (Opuntia elatior Mill.). Kovalen, 2017; 3(2):142-149.
59. Kurniati N, Prasetya AT, & Winarn. Ekstraksi dan uji stabilitas zat warna brazilien dari kayu secang (Caesalpinia sappan L.,). Indonesian Journal of Chemical Science, 2012; 1(1).
60. Rundubello BA, Ridhay A, Hardi J, & Puspitasari DJ. Uji stabilitas pigmen esktrak ubi banggai (Dioscoreabulbifera var celebica Burkill) pada berbagai variasi pH dan lama paparan sinar matahari. KOVALEN: Jurnal Riset Kimia, 2019; 5(1):9-16.
61. Gao Y, Liu Y, Kan C, Chen M, & Chen J. Changes of peel color and fruit quality in navel orange fruits under different storage methods. Scientia Horticultura, 2019;256:108522.
62. Sagdic O, Ekici L, Ozturk I, Tekinay T, Polat B, Tastemur B, Bayram O, & Sentruck B. Cytotoxic and bioactive properties of different color tulip flowers and degradation kinetic of tulip flower anthocyanins. Food and Chemical Technology, 2013; 58:432-439.
63. Zhang Z, Li J, & Fan L. Evaluation of the composition of Chinese bayberry wine and its effects on the color changes during storage. Food Chemistry, 2018; 276:451-457.
64. Kechinski CP, Guimaraes PVR, Norena CPZ, Tessaro IC, & Marozak LDF. Degradatiom kinetics of anthocyanins in blueberry juice during thermal treatment. Journal of Food Science, 2010; 75(2): C173-176.
65. Jimenez N, Bohuon P, Lima J, Dornier M, Vailnat F, & Perez AM. Kinetics of anthocyanins degradation and browning in reconstituted blackberry juice treated at high temperaturs (100-180 C). Journal of Agricultural and Food Chemistry, 2010; 58:2314-2322.
66. Melgarejo P, Martinez R, Hernandez F, Martinez JJ, & Legua P.. Anthocyanin content and color development promagnate jam. Food and Bioproduct Technology, 2011; 89:477-481.
67. Buve C, Kebede BT, Batseller CD, Carrilo C, Pham HTT, Hendrick M, Grauwet T, & Loey AV. Kinetics of colour changes in pasteurised strawberry juice during storage. Journal of Food Engineering, 2017; 216:42-51.
68. Wang Z, Zhang M, & Wu Q. Effects of temperature, pH, and sunlight exposure on the color stability of strawberry juice during processing and storage. LWT-Food Science and Technology, 2014; 60(2):1174-1178.
69. Wibowo S, Vervoort L, Tonic J, Santiago JS, Lemmens L, Pannozo A, Grauwet T, Hendrikx M, & Loey A. Colour and carotenoid changes of pasteurised orange juice during storage. Food Chemistry, 2015; 171; 330-340.
70. Shao-qian C, Luang L, & Si-yi P. (2011). Thermal degradation kinetics of anthocyanins and visual color of blood orange juice. Agricultural Science, 2011; 10(12):1992-199.
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How to Cite
Armalina, Y., Azizah, Z., & Asra, R. (2020). The Degradation Rates of Natural Dyes from Natural Resources: A Review. Asian Journal of Pharmaceutical Research and Development, 8(5), 75-81.