The Antihyperglycemic Activity of Sea Cucumber (Stichopushermanii) Ethanol Extract Against White Male Rats (Rattus novergicus)
Objectives: The purpose of this study was to determine antihyperglycemic activity of Sea Cucumber (Stichopushermanii) ethanol extract on white male rats, by observing changes in blood glucose levels after being induced by streptozotocin.
Design:The research method used was experimental laboratory, where as test animals were divided into several groups given the test substance. The tests performed on animals were glucose tolerance test and antihyperglycemia test after being induced by streptozotosin.
Interventions:Test animals that have been grouped into each group were given 0.5% Na CMC suspension as a negative control, metformin 65 mg / kg bw as a positive control, extracts of 50, 100, 200, 400 and 800 mg / kg bw as test samples.
Main outcome measure:The results showed good results at a dose of 800 mg / kg bw in the 120th minute on the glucose tolerance test with blood glucose levels of 98.33 + 7.02, and the antihyperglycemic activity test after the rats were induced by streptozotocin with blood glucose levels of 65.84 + 2.93 on the 15th day. This value was not significantly different from the positive control (metformin) with a p value> 0.05.
Conclusion:Ethanol extract of sea cucumber (Stichopushermanii) has antihyperglycemic activity at a dose of 800 mg / kg bw.
2. Prawitasari DS, Safitri I, Notopuro H. Effects of Golden Sea Cucumber Extract (Stichopus hermanii) on Fasting Blood Glucose, Plasma Insulin, and MDA Level of Male rats (Rattus novergicus) Induced with Streptozotocin. Fol Med Indones. 2019;55(2):107–11.
3. Windari HAS, Tarman K, Safithri M, Setyaningsih I. Antioxidant Activity of Spirulina platensis and Sea Cucumber Stichopus hermanii in Streptozotocin-Induced Diabetic Rats. Trop Life Sci Res. 2019;30(2):119–29.
4. Nayareh P, Hasan MK. Effect of Chromium Supplementation on Blood Glucose, Hemoglobin A1C, Lipid Profile and Lipid Peroxidation in Type 2 Diabetic Patients. Iran J Diabetes Obes. 2012;4(4):178–84.
5. Slater MJ, Lassudrie M, Jeffs AG. Method for Determining Apparent Digestibility of Carbohydrate and Protein Sources for Artificial Diets for Juvenile Sea Cucumber, Australostichopus mollis. J World Aquac Soc. 2011;42(5):714–25.
6. Kew PL, Don MM. Jewel of the Seabed: Sea Cucumbers as Nutritional and Drug Candidates. Int J Food Sci Nutr. 2012;63(5):616–36.
7. Xu C, Zhang R, Wen Z. Bioactive Compounds and Biological Functions of Sea Cucumbers as Potential Functional Foods. J Funct Food. 2018;49:73–84.
8. Khotimchenko Y. Pharmacological Potential of Sea Cucumbers. Int J Mol Sci. 2018;19(5):1342.
9. Meliani N, El Amine Dib M, Allali H, Tabti B. Hypoglycaemic Effect of Berberis vulgaris L. in Normal and Streptozotocin-Induced Diabetic Rats. Asian Pac J Trop Biomed. 2011;1(6):468–71.
10. Elekofehinti OO, Kamdem JP, Kade IJ, Adanlawo IG, Rocha JBT. Saponnins from Solanum anguivi Lam. Fruit Exhibit In Vitro and In Vivo Antioxidant Activities in Alloxan-Induced Oxidative Stress. Asian J Pharm Clin Res. 2013;6(2):249–54.
11. Husni A, Ismed F, Afriyandi D. Standardization Study of Simplicia and Extract of Calamondin (Citrus microcarpa Bunge) Peel, Quantification of Hesperidin and Antibacterial Assay. Pharmacogn J. 2020;12(4):777–83.
12. Yulianingtyas A, Kusmartono B. Optimization of Solvent Volume and Maceration Time on Extraction of Flavonoids from Averrhoa bilimbi Leaves. J Tek Kim. 2016;10(2):58–64.
13. Azwanida NN. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants. 2015;4(196):412–2167.
14. Capdevilla S, Giral M, Ruiz de la Torre L. Acclimatization of Rats after Ground Transportation to a New Animal Facility. Lab Anim. 2007;41:255–61.
15. Langley SC, Browne RF, Jackson AA. Altered Glucose Tolerance in Rats Exposed to Maternal Low Protein Diets in Utero. Comp Biochem Physiol Part A Physiol. 1994;109(2):223–9.
16. Islam A, Akhtar A, Khan RI, Hossain S, Alam K, Ibne Wahid I, et al. Oral Glucose Tolerance Test (OGTT) in Normal Control and Glucose Induced Hyperglycemic Rats with Coccinia cordifolia L. and Catharanthus roseus L. Pak J Pharm Sci. 2009;22(4):402–4.
17. Muruganadan S, Srinivasan K, Gupta S, Gupta PK, Lal J. Effect of Mangiferin on Hyperglycemia and Atherogenicity in Streptozotocin Diabetic Rats. J Ethnopharmacol. 2005;97(3):497–501.
18. Rasch R. Prevention of Diabetic Glomerulopathy in Streptozotocin Diabetic Rats by Insulin Treatment. Diabetologia. 1979;16:319–24.
19. Pringgenies D, Rudiyanti S, Yudiati E. Exploration of Sea Cucumbers Stichopus hermanii from Karimunjawa Islands as Production of Marine Biological Resources. In: IOP Conference Series: Earth and Environmental Science. 2018. p. 1–8.
20. Arulselvan P, Subramanian SP. Beneficial Effects of Murraya koenigii Leaves on Antioxidant Defense System and Ultra Structural Changes of Pancreatic β-cells in Experimental Diabetes in Rats. Chem Biol Interact. 2007;165(2):155–64.
21. Tiong SH, Looi CY, Hazni H, Arya A, Paydar M, Wong WF, et al. Antidiabetic and Antioxidant Properties of Alkaloids from Catharanthus roseus (L.) G. Don. Molecules. 2013;18(8):9770–84.
22. Elekofehinti OO. Saponins: Anti-Diabetic Principles from Medicinal Plants – A Review. Pathophysiology. 2015;22(2):95–103.
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