Characterization and Anti-Inflammatory Activity of Ethanol Extract of Sikkam (Bischofia Javanica Blume) Stem Bark

  • Aswan Pangondian Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Marline Nainggolan Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Aminah Dalimunthe Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

Abstract

One of the medicinal plants that have been known since ancient times is sikkam (Bischofia javanica Blume) which is used as a natural dye in rattan and bamboo matting. Lawang Agung Village Community Kab. Lahat South Sumatra uses sikkam as a spice in cooking which is believed to be anti-inflammatory.  This study aimed to assess the anti-inflammatory activity of ethanol extract of sikkam stem bark (Bischofia Javanica Blume) to reduce the edema volume of male white rats induced by carrageenan 1% and also to determine the effective dose of extract to reduce the volume of rat foot edema.Ethanol extract of sikkam bark (Bischofia Javanica Blume) was obtained by maceration. Anti-inflammatory activity test was divided into 5 groups. Group I (negative control) was given 0.5% CMC, Group II (positive control) was given diclofenac sodium 2.25 mg /kgBW, while Group III, IV and V were given ethanol extracts of sikkam bark with doses of 50,100 and 200 mg/kgBW respectively. Each rat was induced by 1% carrageenan subplantar injection. Examination of anti-inflammatory effects was measured using a digital plethysmometer at 30 to 360 minutes. Data were analyzed statistically using ANOVA (analysis of variance). The results showed that negative controls did not show an anti-inflammatory effect that had a significant difference with the other treatment groups. In conclusion, the ethanol extract of sikkam bark (Bischofia Javanica Blume) has effective anti-inflammatory activity at a dose of 200 mg/kgBW.

Keywords: Anti-inflammatory, Bischofia javanica Blume, flavonoids.

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Author Biographies

Aswan Pangondian, Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

Marline Nainggolan, Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

Aminah Dalimunthe, Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

 Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

References

1. han T.A, Anjaria J.K, Vanish D, Gohel A.K.. Bioactivity Guided Fractination and Antiinflammatory activity of Acacia Nilotica Pods. International Journal of Pharmacy and Pharmaceutical Sciences, 2015;7(7):380-3
2. Bachheti R.K., Indra R & Archana J. Chemical Composition, Mineral and Nutritional Value of Wild Bischofia javanica seed. Intenasional Food Research Journal, 2013; 20(4):1747-1751
3. Harmida, Sarno & Yuni V.F . Ethnophytomedic Study in Lawang Agung Village, Mulak Ulu District, Lahat Regency, South Sumatra. Jurnal Penelitian Sains, 2011; 14(1)
4. Otshudi L.A., Vercruysse A., & Foriers A. Contributions to Ethnobotanical, Phytochemical and Pharmacological Studies of Traditionally Used Medical Plant in the Treatment of Dysentery and Darrhoea in Lomela Area, Democratic Republik of Congo (DRS), Journal of Ethonopharmacol, 2000; 71(3):411
5. Winarti, C and Nurdjanah N. Opportunities for spices and medicinal plants as functional food sources. Jurnal Litbang Pertanian, 2005; 24(2):47-55
6. Ajaib, M. and Khan, Z.U. Bischofia javanic: A New Record to The Flora of Pakistan. Pakistan: Biologia. 2012; 58(1,2): 179-183.
7. Jambak K, Nainggolan M & Dalimunthe A. Antioxidant Activity of Ethanolic Extract and N-Hexane Fraction from Sikkam (Bischofia javanica Blume) Stem Bark. Asian Journal of Pharmaceutical Research and Development.2019;7(2):01-05
8. Levya-Lopez N, Gutierrez-Grijalva E.P, Ambriz-Perez D.L & Heredia JB. Flavonoids as Cytokine Modulators A Possible Therapy For Inflammation-Related Diseases. International Journal Molecular sciences. 2016; 17(6):921;
9. Directorate General of The Drug and Food Regulatory Agency. Indonesian Materia Medica 6th Edition. Jakarta: Republic of Indonesia Ministry of Health; 1995.p.299-303,321, 333-338.
10. Farmsworth NR. Biologycal and Phytochemical Screening of Plants. Journal of Pharmaceutical Science. 1966; 55(3):262-264
11. Harbone JB. Phytochemical Method, Modern Ways Guide to Analyze Plants. Kosasih Padmawinata translator, 2nd Edition. Bandung: ITB Press; 1987. P.6-8, 104, 152
12. Vogel H.G., Bernward A.S, Jurgen S., Gunter M., & Wolfgang F.V. Drug Discovery and Evaluation Pharmacological Assay. 2nd Ed. New York: Springer-Verlag Berlin Heidelberg. 2008 p. 759-760, 767-768.
13. Fehrenbacher JC, Vasko MR, Duarte DB. Models of inflammation: Carrageenan- or complete Freund's Adjuvant (CFA)-induced edema and hypersensitivity in the rat. Curr Protoc Pharmacol. 2012;Chapter 5:Unit 5.4.
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Pangondian, A., Nainggolan, M., & Dalimunthe, A. (2020). Characterization and Anti-Inflammatory Activity of Ethanol Extract of Sikkam (Bischofia Javanica Blume) Stem Bark. Asian Journal of Pharmaceutical Research and Development, 8(4), 16-20. https://doi.org/https://doi.org/10.22270/ajprd.v8i4.779