Screening of Preliminary Phytochemicals and GC-MS Analysis of Chloroform Extract of Chaetomorpha media (C.Ag.) Kuetzing

Authors

  • Anusathya Priya P Research Scholar, Centre for Advanced Research in Plant Sciences (CARPS), Department of Botany, St.Xavier’s  College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.
  • John Peter Paul J Assistant Professor of Botany and Director, Centre for Advanced Research in Plant Sciences (CARPS), St.  Xavier’s College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.
  • Iniya Udhaya C Assistant Professor, Department of Botany, Sri Kaliswari College (Autonomous), Sivakasi - 626 123, Tamil  Nadu, India.
  • Fredrick Raja E Research Scholar, Centre for Advanced Research in Plant Sciences (CARPS), Department of Botany, St.Xavier’s  College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.
  • Ponnudurai G Research Scholar, Centre for Advanced Research in Plant Sciences (CARPS), Department of Botany, St.Xavier’s  College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.
  • Muthu Sheeba M Research Scholar, Centre for Advanced Research in Plant Sciences (CARPS), Department of Botany, St.Xavier’s  College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.

DOI:

https://doi.org/10.22270/ajprd.v8i4.773

Keywords:

Marine macro green algae, Phytochemicals, Chaetomorpha media, Chloroform extract, GC-MS.

Abstract

Objectives: The present study was carried out to analyse the chloroform extract of marine green macro alga Chaetomorpha media (C.Ag.) Kuetzing, belonging to the family Chlorophyceae. 

Design: The plant materials were collected from Manapad, located in Thoothukudi district, Tamil Nadu, India. The preliminary phytochemical analysis was carried out by Harborne method and followed by the characterization of biochemicals were predicted using GC-MS analysis.

Intervention: Among the various solvent extracts, chloroform extract was intervened in this study

Main outcome measure: The main measurement results in this study were to determine the presence of various phytochemicals in the chloroform extract of Chaetomorpha media (C.Ag.) Kuetzing

Results: In the preliminary phytochemical analysis of the chloroform extract of Chaetomorpha media (C.Ag.) Kuetzing, seventeen different types of secondary metabolites such as alkaloids, anthocyanin, anthraquinone, catechin, cardiac glycosides, coumarins, flavonoids, glycosides, phenolic groups, phlobatannins, phytosteroids, quinones, saponins, tannins, terpenoids, emodins and diterpenes were present in the chloroform extract. GC-MS spectrum of chloroform extract of Chaetomorpha media (C.Ag.) Kuetzing revealed 18 different major peaks which indicated the presence of eighteen compounds. The prevailing compounds in chloroform extract were 6-Methyloctahydro coumarin, Cyclododecane methanol, 2,3,4-Trimethoxyphenyl acetonitrile, Silicic acid, diethyl bis(trimethylsilyl) ester, Propanamide, 3-bromo-N-(4-bromo-2-chlorophenyl), 2-Ethylacridine, Bis(2-ethylhexyl) phthalate, Silane, trimethyl [5-methyl-2-(1-methylethyl)phenoxy], 1H-Indole, 1-methyl-2-phenyl, Benzo[h]quinoline, 2,4-dimethyl, Indole-2-one, 2,3-dihydro-N-hydroxy-4-methoxy-3,3-dimethyl, Tris(tertbutyl dimethylsilyloxy) arsane,  [1,2,4]-Triazolo[4,3-a] [1,3,5]-triazine 5,7-diacetylamino-3-methyl, Cyclotrisiloxane, hexamethyl, Benzo[h]quinoline, 2,4-dimethyl,  N,N-Dimethyl-4-nitroso-3-(trimethylsilyl) aniline,  2-Methyl-7-phenylindole, 1,1,1,3,5,5,5-Heptamethyltrisiloxane.

Conclusion: The chloroform extract of Chaetomorpha media (C.Ag.) Kuetzing showed the presence of various phytochemicals which can be used as medicine to cure various diseases.

 

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Author Biographies

Anusathya Priya P, Research Scholar, Centre for Advanced Research in Plant Sciences (CARPS), Department of Botany, St.Xavier’s  College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.

Research Scholar, Centre for Advanced Research in Plant Sciences (CARPS), Department of Botany, St.Xavier’s

 College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.

John Peter Paul J, Assistant Professor of Botany and Director, Centre for Advanced Research in Plant Sciences (CARPS), St.  Xavier’s College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.

Assistant Professor of Botany and Director, Centre for Advanced Research in Plant Sciences (CARPS), St.

 Xavier’s College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.

Iniya Udhaya C, Assistant Professor, Department of Botany, Sri Kaliswari College (Autonomous), Sivakasi - 626 123, Tamil  Nadu, India.

Assistant Professor, Department of Botany, Sri Kaliswari College (Autonomous), Sivakasi - 626 123, Tamil

 Nadu, India.

Fredrick Raja E, Research Scholar, Centre for Advanced Research in Plant Sciences (CARPS), Department of Botany, St.Xavier’s  College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.

Research Scholar, Centre for Advanced Research in Plant Sciences (CARPS), Department of Botany, St.Xavier’s

 College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.

Ponnudurai G, Research Scholar, Centre for Advanced Research in Plant Sciences (CARPS), Department of Botany, St.Xavier’s  College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.

Research Scholar, Centre for Advanced Research in Plant Sciences (CARPS), Department of Botany, St.Xavier’s

 College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.

Muthu Sheeba M, Research Scholar, Centre for Advanced Research in Plant Sciences (CARPS), Department of Botany, St.Xavier’s  College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.

Research Scholar, Centre for Advanced Research in Plant Sciences (CARPS), Department of Botany, St.Xavier’s

 College (Autonomous), Palayamkottai - 627 002, Tamil Nadu, India.

References

1. Bhakuni DS, Rawat DS. Bioactive Marine Natural Products. New Delhi, Anamaya Publishers. 2005:382.
2. Gajalakshmi D, Shettu N, Murugesan S. Phytochemical screening of marine red alga Botryocladia leptopoda (J.Agardh) Kylin, International Journal of Interdisciplinary Research and Innovations. 2018; 6(2):463-70.
3. Deepa S, Bhuvana B, Hemamalini S, Janet C, Sathesh Kumar K. Therapeutic potential and pharmacological significance of the marine algae Gracilaria corticata, Pharmaceutical and Biological Evaluations. 2017; 4(2):68-72.
4. Mohana Priya K, Shabana Kouser A. Antibacterial activity of aqueous extract of seaweed Ulva fasciata: An in vitro study. International


5. Journal of Current Pharmaceutical Review and Research. 2011; 2(2):101-5.
6. Priscila IU, Mariama TNS, Luiz CDS, Luciano B, Ary F. Antibacterial activity of medicinal plant extracts. Brazilian Journal of Microbiology. 2007; 38:717-19.
7. Chiara L, Jeanette H, Andersen EH, Marte A, Laura E, Francesco E, Kirsti H, Kine H, Giovanna R, Adrianna I. Bioactivity screening of microalgae for antioxidant, anti-inflammatory, anticancer, anti-diabetes and antibacterial activities. Frontiers in marine science. 2016; 3:68.
8. Hanumantha R, Balaji R, Arumugam S, Thiruvengadam D. Hepatoprotective nature of seaweed alcoholic extract on acetaminophen induced hepatic oxidative stress, Journal of Health Science. 2004; 50(1):42-6.
9. Muthu Sheeba M, John Peter Paul J. CNS depressant activity of methanolic extract of Chaetomorpha litorea Harvey (Green Seaweed) in Kanyakumari Coast, Tamil Nadu, India, Journal of Emerging Technologies and Innovative Research. 2019; 6(3):240-43.
10. Maharashtra MMN, Sabale AB. Antioxidant potential of seaweeds from Kunakeshwar along the West Coast. Asian Journal of Biomedical & Pharmaceutical Sciences. 2013; 3(22):45-50.
11. John Peter Paul J, Patric Raja D. Seaweeds of the south coast of Tamil Nadu, India. 2014. P.52.
12. Iniya Udhaya C, John Peter Paul J. Screening of preliminary phytochemicals of Gracilaria cylindrica Boergesen in Koothankuzhi, Tirunelveli district, Tamil Nadu, India. Indo American Journal of Pharmaceutical Sciences. 2017; 4(12):4590-4.
13. Iniya Udhaya C, John Peter Paul J. Screening of anti-oxidant activity of methanolic extract of Gracilaria fergusonii J.Ag. (Red seaweed) in Hare Island, Thoothukudi, Tamil Nadu, India. Indo American Journal of Pharmaceutical Sciences, 2017; 4(9):2724-7.
14. Harborne JB. Phytochemical Methods: A guide to modern techniques of plants analysis, Chapman & Hall. London, Ltd. 1998. P.49-188.
15. John Peter Paul J. GC-MS analysis of acetone extract of Caulerpa racemosa (Forssk.) Web. V. Bosse collected from Kanyakumari in the south east coast of Tamil Nadu, India. Journal of Drug Delivery & Therapeutics. 2019; 9(4):571-3.

Published

2020-08-15

How to Cite

P, A. P., Paul J, J. P., C, I. U., E, F. R., G, P., & M, M. S. (2020). Screening of Preliminary Phytochemicals and GC-MS Analysis of Chloroform Extract of Chaetomorpha media (C.Ag.) Kuetzing. Asian Journal of Pharmaceutical Research and Development, 8(4), 37–40. https://doi.org/10.22270/ajprd.v8i4.773