In-vitro Antioxidant and Free Radical Scavenging Activity of A 50% Hydroethanolic Polyherbal Crude Extract
The multiple activities of the plant based medicinal preparations meant for varieties of inflammatory diseases offer enormous scope for combating the threat of diseases. The present study was designed to develop safer, effective and viable anti-inflammatory herbal combination to control oxidative stress related ailments as new alternatives to synthetic drugs. The synergistic potential of herbal combination of two medicinal plants was assessed through curative and preventive mode of treatment. Among the numerous traditional herbs, Gymnea sylvestre and Urgenia Indica is widely used for its traditional value. Free radicals induce numerous diseases by lipid peroxidation, and DNA damage. It has been reported that numerous plant extracts have antioxidant activities to scavenge free radicals. In the present study, the antioxidant properties of crude 50 % hydroethanolic polyherbal crude extract of Gymnea sylvestre and Urgenia Indica were studied in in-vitro models viz. radical scavenging activity by DPPH reduction Assay, Scavenging of SO, H2O2 and reducing power, DOT PLOT and FRAP assay. The extract was found to contain large amounts of phenolic compounds and flavonoids. 50% hydroethanolic polyherbal crude extract of Gymnea sylvestre and Urgenia Indica possessed significant antioxidant activity. These results suggest that synergistic activity of the crude extract has potential to develop a new functional dietary agent to treat chronic metabolic diseases, such as diabetes , hyperlipidemia and various inflammatory diseases.
2. Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology. 2005; 4(7):685–8.
3. Srivastava. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods of Enzymol., 2013; 299:152-178.
4. Jayakumar and Parasraman., Scavenging Capacity of Superoxide Radical and Screening of Antimicrobial Activity of Castanea sativa Mill. Extracts. Czech. J. Food. Sci. 2010; 28(1):61-68
5. Satheesh Kumar bhandary, S., Biswas, A., Dhali, G. K., Chowdhury, A., Boyer, J. L., and Santra, A.Oxidative pressure and hepatic stellate cell initiation are entering occasions in arsenic instigated liver fibrosis in mice. Toxicology and connected pharmacology, 2012; 251(1):59-69.
6. Chandrasekar, York DA, Rössner S, Hubbard V, Caterson I,Hayman LL et al. American Heart Association: Prevention conference VII obesity, a Worldwide epidemic related to heart disease and stroke: executive summary. Circulation 2006; 110:2968–2975.
7. Manohar SH, Naik PM, Praveen N, Murthy HN. Distribution of gymnemic acid in various organs of Gymnema sylvestre. J Forestry Res. 2009; 20(3):268-70.
8. Singh VK, Umar S, Ansari SA, Iqbal M. Gymnema sylvestre for diabetics. J Herbs Spices Medici Plants. 2008; 14(1-2):88-106.
9. Deb DB , Dasgupta S: Studies on Indian squill Urginea indica.Journal of Crude Drug Research 1976; 14:49- 60.
10. Kirtikar K R, Basu BD:, International Book Distributors Book sellers and Publishingers, Dehradun, Indian Medicinal Plants.Vol.4:1999.
11. Harini SS, Leelambika M, Shiva Karmeswari MN, Satyanarayana N: Optimization of DNA isolation and PCR-RAPD methods for molecular analysis of Urginea indica (Kunth). International Journal of Integrative Biology 2008; 2(2):138-144
12. Chen Z, Bertin R & Froldi G, ECestimation of antioxidant activity in DPPH assay using several statistical programs, Food Chem, 2013; 138(1):414-20.
13. Sahu Rajeshwari & Saxena Jyoti, Screening of total phenolic and flavonoid content in conventional and non-conventional species of curcuma, J Pharmaco Phytochem,2013; 2(1):176-179
14. 14.John B, Sulaiman CT, George S & Reddy VRK, Spectrophotometric estimation of total alkaloids in selected Justicia species, Int J Pharm Pharm Sci,2014; 6(5):647-648
15. Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I. Antioxidant principles from Bauhinia terapotensis. J Natur Prod. 2001; 64(7):892-5.
16. Liu F, Ooi VE, Chang ST. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci. 1997;60(10):763-71.
17. Nabavi S, Ebrahimzadeh M, Nabavi S, Hamidinia A, Bekhradnia A. Determination of antioxidant activity, phenol and flavonoids content of Parrotia persica Mey. Pharmacology Online. 2008; 2:560-7
18. Benzie I F F, Strain JJ: Ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal Biochem 1996, 239:70-7.
19. Chang.R.,Fedekar, M. F., and Abdel-Daim, M. M.andGarret.A.F.PG/and Inflammation. Arteriosclerosis Thrombosis Vascular Biology, 2002; 31, 986-1000.
20. Satheesh Kumar bhandary, S., Biswas, A., Dhali, G. K., Chowdhury, A., Boyer, J. L., and Santra, A. Oxidative pressure and hepatic stellate cell initiation are entering occasions in arsenic instigated liver fibrosis in mice. Toxicology and connected pharmacology, 2012; 251(1):59-69.
21. Sengupta Rupa, P. V., Shire, P. S., Nazim, S., and Choudhari, A. B. Advancement and assessment of polyherbal plan for mitigating movement. J Nat Prod Plant Resour, 2016; 1:85-90.
22. VinothaShanmugarajah., and Ripoll, C. Can an apple daily ward off the specialist. Current Pharmaceutical Design, 2013; 10(27):3419-3429.
23. Sundey J Ameh, E. S., Rao, P. R., Rao, B. G., and Rao, T. M. Assessment of HPA of Gynandropsisgynandra. Diary of Pharmacy Research, 2010; 6(9):928-932.
24. Mehdi Zarei, Ghante MH, Upaganlawar AM, Yeole PG. Antihyperlipidemic effect of the methanolic extract from Lagenaria siceraria Standard fruit in hyperlipidemic rats. Journal of Ethnopharmacology 2010; 124(2):333-337.
25. Sengupta , Oshodi AA. Proximate Composition and Some Functional Properties of Three Varieties of Lagenaria siceraria Melon Seeds. Research Journal of Agriculture and Biological Sciences 2014; 6(2):108-112.
26. Croteau, K. C., Ramesh, An., and Mohan, G. K. (2000). Hepatoprotective and Antioxidant Effects of MucunaPruriens against Acetaminophen-Induced Hepatotoxicity in Albino Wister Rats. Research Journal of Pharmacy and Technology, 2014; 7(1):70-73.
27. Olemy, Upadhya L, Singh RN. Effect of Terminalia arjuna on lipid profile of rabbit fed hypercholesterolemic diet. International Journal of Crude Drug Research 2014; 28:48–51.
28. Garrat, Pulichino, A. M., Rowland, S., Wu, T., Clark, P., Xu, D., Mathieu, M. C., and Audoly, L. P. Prostacyclin threat diminishes torment and aggravation in rat models of hyperalgesia and perpetual joint inflammation. Diary of Pharmacology and Experimental Therapeutics, 2016; 319(3), 1043-1050.
29. Sawarkar, F. M., Levitsky, D. O., and Dembitsky, V. M. Aziridine alkaloids as potential remedial specialists. European diary of restorative science, 2009; 44(9), 3373-3387.
30. Narayanasamy and Ragavan. (2012). Difficulties and openings in tranquilize revelation from plants. Current science, 1251-1257.
31. Patel, S. J., Nimbalkar, S. S., Kulkarni, A. D., and Madhavi, D. L. (2011). Lipid oxidation in natural and nourishment frameworks. Nourishment.Pietta and Elliss, P. DP and DB: A world wide scenario. DB, Res. and Clini. Practice, 2000; 88:303-311.
32. Gulcin. Hypocolesteremic Effects of T. arjuna tree bark. Journal of ethonopharmacology 2016; 55:165-169.
33. Kosanic,Patel MB, Rai PD, Mishra SH. Assessment of anti-oxidant and wound healing potential of Eclipta alba. Centella asiatica and their combination with Piper nigrum 2011; 9(1):21-26.
34. Parejo., Joseph Oyepata, S., EmemEkpo, U.HPA of the concentrate of Homaliumletestui stem against paracetamol-prompted liver damage. Avicenna J Phytomed, 2002; 7(1):27– 36.
35. Ganapathy. An., Angadi, N. B., and Matule, S. M. Impact of dipeptidyl peptidase 4 inhibitors on intense and subacute models of aggravation in male Wistar rats: A test think about. Worldwide Journal of Applied and Basic Medical Research, 2014; 7(1), 26.
36. Patel, S. J., Nimbalkar, S. S., Kulkarni, A. D., and Madhavi, D. L. Lipid oxidation in natural and nourishment frameworks. Nourishment Akin Pelu,Kaur G, Kulkarni SK. Antiobesity effect of a polyherbal formulation, OB-200G in female rats fed on cafeteria and atherogenic diets. Indian Journal of Pharmacology 2010; 32:294-299.
37. Sadowska Woda, Singh RB, Niaz MA, Ghosh S. Hypolipidemic and antioxidant effects of Commiphora mukul as an adjunct to dietary therapy in patients with hypercholesterolemia. Cardiovas. Drugs Ther 2010; 8:659-64.
38. Govindarajan.,Kaur, S., Kumar, S., and Singh, P. Rubiacordifolia L. what's more, Glycyrrhizaglabra L. restorative plants as potential wellspring of COX-2 inhibitors. Am J Biomed Sci, 2004; 2(2):108-120.
39. Mir, N., Shakiba, Y., and Mahmoudi, M. CLand oxidative worry as a noteworthy reason for age-related maladies and disease. Late licenses on aggravation and sensitivity tranquilize revelation, 2013; 3(1):73-80.
40. Sharma, O. E., Olayemi, F. O., Ning-Hua, T., and Guang-Zhi, Z. In vitro cell. Reinforcement movement, add up to phenolic and flavonoid substance of ethanol concentrate of stem and leaf of Grewiacarpinifolia. Beni-Suef University Journal of Basic and Applied Sciences, 2013; 6(1), 10-14.
41. Katyayani.,Hari, R., Vasuki, R., and Geetha, HPA of Bi-home grown ethanolic separate on CCl4 actuated hepatic harm in rats. African Journal of Biochemistry Research, 2012; 2(2):061-065.
42. Atgulcin, Jackson HC, Vickers SP, Heal DJ, Dickinson K, Jones RB et al. Novel targets for the treatment of obesity: a review of progress. Drug Discovery today: Therapeutic strategies 2008; 1(2):227-235.
43. Aboul- Enein Williamson EM, Okpako DT. Selection, Preparation and Pharmacological Evaluation of Plant Material John Wiley and Sons, 2003, 1-3.
44. Deepa Babu., and Olorunnisola, O. S. Hepatoprotective capability of some neighborhood Medicinal Plants against 2-Acetylaminoflourene-incited harm in rodent Diary of toxicology, 2013; 4(2):15-19.
45. Rajkumar ,Zamani M, Alireza O, Sarker SK. Assessment of anti-hyperlipidemic effect of Citrullus colocynthis. Brazilian . Journal of Pharmacognosy 2008; 17(4):492-496.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
AUTHORS WHO PUBLISH WITH THIS JOURNAL AGREE TO THE FOLLOWING TERMS:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).