Antidiarrheal Activity of n-Hexane Fraction Seeds of Leucaenae leucocephala (Lam) de Wit on Rat

  • Saddam Husein Department of Pharmacy Biology, Faculty of Pharmacy, University of Sumatera Utrara, Medan, Sumatera  Utara, Indonesia
  • Marline Nainggolan Department of Pharmacy Biology, Faculty of Pharmacy, University of Sumatera Utrara, Medan, Sumatera Utara, Indonesia
  • Yuandan Y Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Sumatera Utara, Indonesia

Abstract

Objective: To evaluate the antidiarrheal activity of the n-hexane fraction of L. leucocephala (Lam) de Wit seeds induced by castor oil and intestinal motility methods for rats.


Method: L.leucochepala seeds simplicia powder extracted by maceration with 80% ethanol. Ethanol extract was fractionated with n-hexane solvent. Investigating of antidiarrheal activity of L.leucocephala fraction by induction of castor oil and intestinal motility methods.


Result: The n-hexane L.leucocephala fraction reduced diarrhea based on observations of the onset of diarrhea, frequency, consistency, weight of stool and duration of diarrhea compared with negative controls. The n-hexane L.leucocephala fraction showed antidiarrheal activity at doses of 200 and 400 mg/kg  bw (P <0.05) but differed significantly from loperamide (P <0.05). In the intestinal motility method of the n-hexane fraction doses of 200 and 400 mg/kg bw the activity in inhibiting intestinal motility was significantly different from 0.5% CMC Na (P <0.05).


Conclusion: Then-hexane fraction of L. leucocephala seeds based on antidiarrheal activity testing has the ability to reduce diarrhea.


 


 

Keywords: Leucaena leucocephala, antidiarrhea, castor oil induced diarrhea, intestinal motility

Downloads

Download data is not yet available.

Author Biographies

Saddam Husein, Department of Pharmacy Biology, Faculty of Pharmacy, University of Sumatera Utrara, Medan, Sumatera  Utara, Indonesia

Department of Pharmacy Biology, Faculty of Pharmacy, University of Sumatera Utrara, Medan, Sumatera

 Utara, Indonesia

Marline Nainggolan, Department of Pharmacy Biology, Faculty of Pharmacy, University of Sumatera Utrara, Medan, Sumatera Utara, Indonesia

Department of Pharmacy Biology, Faculty of Pharmacy, University of Sumatera Utrara, Medan, Sumatera

 Utara, Indonesia

Yuandan Y, Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Sumatera Utara, Indonesia

Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Sumatera Utara, Indonesia

References

1. Jeejeebhoy, K. N. Symposium on Diarrhea Definition and Mechanisms of diarrhea. Scientific Section. CMA Journal. 1977; 116(7):737-739.
2. Raj V, Rai A, Singh M. Detection of bioflavonoids from methanol bark extracts of Acacia and their role as antidiarrhoeal agents. J Anal Pharm Res. 2017; 5(4):1–7.
3. Kumar, B., Divakak, K.,Tiwari, P., Salhan, M.,and Goli, D. Evaluation of antidiarrheal effect of aqueous and ethanolic extracts of fruit pulp of Terminalia belerica in rats. Int J Drug Dev Res. 2010; 2(4):769–79.
4. Balaji, G., Chalamaiah, M., Ramesh, B., and Reddy, Y.A. Antidiarrhoeal activity of ethanol and aqueous extracts of Carum copticum seeds in experimental rats. Asian Pac J Trop Med. 2012; 2(2):S1151-S1155.
5. KIM, Hack-Seang. Do not put too much value on conventional medicines. Journal of ethnopharmacology. 2005; 100.1-2:37-39.
6. Karimi, A., Majlesi, M., And Rafieian-Kopaei, M. Herbal versus synthetic drugs; beliefs and facts. J Nephropharmacol. 2015; 4(1): 27-30
7. Gupta, H. K., and P. P. Atreja. "Influence of ferric chloride treated Leucaena leucocephala on metabolism of mimosine and 3-hydroxy 4 (1H)-pyridone in growing rabbits." Animal feed science and technology. 1998; 74(1):45-55.
8. Khamseekhiew, B., Liang, J. B., Wong, C.C., Jalan, Z.A. Ruminal and Intestinal Digestibility of Some Tropical Legume Forages. Asian-Aust. J. Anim. Sci. 2001; 14(3):321-325.
9. Degu, A., Engidaword, E., Shibeshi, W. Evaluation of the anti-diarrheal activity of the leaf extract of Croton macrostachyus Hocsht. ex Del. (Euphorbiaceae) in mice model. BMC Complementary and Alternative Medicine. 2016; 16:379.
10. Meite, S., N’Guessan, J.D., Bahi, C., Yapi, H.F., Djaman, A.J., dan Guina, F.G. Antidiarrheal Activity of The Ethyl Acetate Extract of Morindamorindoides in Rats. Tropical Journal of Pharmaceutical Research. 2009; 8(3):201-207.
11. Bussman, R.W., Glenn, A., And Sharo, D. Antibacterial Activity of Medical Plants of Northen Peru - Can Traditional Applications Provide Leads for modern Science, Indian J. of Traditional Knowledge. 2010; 9(4):742-743.
12. Chanwitheesuk, A., Teerawutgulrag, A., Rakariyatham, N. Screening of Antioxidant Activity and Antioxidant Compounds of Some dible Plants of Thailand. J. Food Chemistry. 2005; 92:491–497.
13. Deodhar, U.P., Paradkar, A.R., Purohit, A.P. (1998). Preliminary evaluation of Leucaena leucocephala seed gum as a tablet binder. Drug Dev. Ind. Pharm. 1998; 2(4)6:577-582.
14. Verma PRP, Balkishen R. Studies on disintegrant action of Leucaena leucocephala seed gum in ibuprofen tablet and its mechanism. Journal of Scientific and Industrial Research. 2007; 66: 550-557.
15. Indonesian herbal pharmacopea. 1sted. Jakarta; Departemen Kesehatan Republik Indonesia, 2008:174
16. Yuandani and Nazira. Evaluation of antidiarrheal effect of Artocarpus altilis (Park.) leaves in mice. Int. Res. J. Pharm. 2018; 9(12):53-56.
17. Awouter F, Niemegeer CJE, Lenaerts FM, Janseen PA. Delay of castor oil diarrhoea in rats: a new way to evaluate inhibitors of prostaglandin biosynthesis. J. Pharm. Pharmac. 1978; 30:41-45.
Statistics
76 Views | 78 Downloads
How to Cite
Husein, S., Nainggolan, M., & Y, Y. (2020). Antidiarrheal Activity of n-Hexane Fraction Seeds of Leucaenae leucocephala (Lam) de Wit on Rat. Asian Journal of Pharmaceutical Research and Development, 8(2), 10-13. https://doi.org/https://doi.org/10.22270/ajprd.v8i2.685