HPLC & It’s Utilization in Disease Diagnosis

  • Aadil Ansari Kota College of Pharmacy, Kota, Rajasthan, India
  • Ravi Gupta Kota College of Pharmacy, Kota, Rajasthan, India
  • Mitaksha Jhanwar Kota College of Pharmacy, Kota, Rajasthan, India
  • Peehu Kaushik Kota College of Pharmacy, Kota, Rajasthan, India
  • Divyanshu Sharma Kota College of Pharmacy, Kota, Rajasthan, India
  • Mohd. Shahid Khan Maharishi Arvind International Institute of Pharmacy, Kota, Rajasthan, India
  • Jagdish Chandra Nagar Kota College of Pharmacy, Kota, Rajasthan, India

Abstract

High Performance Liquid Chromatography is a technique in analytical chemistry used to separate, identify and quantify each component in a mixture. The HPLC is suitable for a variety of clinical applications, including pharmaceutical development, legal application such as detecting the Presence of illicit drugs in urine & blood. High-performance liquid chromatography is a technique introduced for the accurate diagnosis of hemoglobinopathies, thalassemias & estimation of glycosylated hemoglobin. The advantage of the HPLC system is the excellent resolution, reproducibility & quantification of several normal & abnormal hemoglobin resulting in accurate diagnosis of thalassemia syndromes. HPLC technique which is used to separate the component in across the two equivalents phases i.e. stationary phases  is solid or liuqid supported on a solid  which is packed into a column while sample moves in along with mobile phase  is liuqid or gas. The purpose of this study is to evaluate the HPLC technique in diagnosis of thalassemia syndromes and also correlate it with clinicohematological profile in these cases.


 

Keywords: Hemoglobinopathies, Thalassemias, Glycosylated, Resolution, Clinicohematological

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Author Biographies

Aadil Ansari, Kota College of Pharmacy, Kota, Rajasthan, India

Kota College of Pharmacy, Kota, Rajasthan, India

Ravi Gupta, Kota College of Pharmacy, Kota, Rajasthan, India

Kota College of Pharmacy, Kota, Rajasthan, India

Mitaksha Jhanwar, Kota College of Pharmacy, Kota, Rajasthan, India

Kota College of Pharmacy, Kota, Rajasthan, India

Peehu Kaushik, Kota College of Pharmacy, Kota, Rajasthan, India

Kota College of Pharmacy, Kota, Rajasthan, India

Divyanshu Sharma, Kota College of Pharmacy, Kota, Rajasthan, India

Kota College of Pharmacy, Kota, Rajasthan, India

Mohd. Shahid Khan, Maharishi Arvind International Institute of Pharmacy, Kota, Rajasthan, India

Maharishi Arvind International Institute of Pharmacy, Kota, Rajasthan, India

Jagdish Chandra Nagar, Kota College of Pharmacy, Kota, Rajasthan, India

Kota College of Pharmacy, Kota, Rajasthan, India

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How to Cite
Ansari, A., Gupta, R., Jhanwar, M., Kaushik, P., Sharma, D., Khan, M., & Nagar, J. (2020). HPLC & It’s Utilization in Disease Diagnosis. Asian Journal of Pharmaceutical Research and Development, 8(2), 114-116. https://doi.org/https://doi.org/10.22270/ajprd.v8i2.666