ISOLATION AND CHARACTERIZATION COLLAGEN OF PATIN FISH SKIN (Pangasius Sp.)

  • Valentina Girsang Faculty of Pharmacy, Universitas Sumatera Utara,Medan, Indonesia
  • Julia Reveny Faculty of Pharmacy, Universitas Sumatera Utara,Medan, Indonesia
  • Marline Nainggolan Faculty of Pharmacy, Universitas Sumatera Utara,Medan, Indonesia

Abstract

Objective: This study aims to isolate and characterize the collagen isolated from the skin of patin fish.Method: Collagen isolation consisted of three steps, namely deproteinization process using NaOH solution concentration of 0,05 M and long soaking time for 3x24 hours; immersion in CH3COOH solution with a concentration of 0,05 M and long soaking time 3x24 hours; Furthermore, collagen was extracted with water at 40°C for 2 hours. The isolated collagen was tested for characteristics including chemical and physical properties compared to commercial collagen.Results: Chemical characteristics of collagen isolated from patin fish skin isolation compared to commercial collagen, respectively: 6,55% and 6,68% water content; ash content of 0,19% and 0,18%; protein content 93,63% and 97,42% and fat content 0,41% and 0,30%; The metal content in the collagen isolated from the skin of patin fish and commercial collagen is below the threshold (Pb); 0,5 mg/kg. Amino acid test results from patin fish skin collagen obtained glycine (234381,88 mg/kg), proline (109404,17 mg/kg), alanine (83988,79 mg/kg), arginine (80918,44 mg/kg) and glutamate (87315,88 mg/kg). The physical characteristics of the patin fish skin collagen showed the presence of amide group A (3286,7cm-1), amide B (2947,23cm-1), amide I (1651,07cm-1), amide II (1450,47cm-1), and amide III (1246,02 cm-1).Conclusion: Patin fish skin can be isolated into collagen that meets the quality requirements of collagen as a cosmetic preparation. 

Keywords: patin fish, isolation, characterization, collagen, skin

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Author Biographies

Valentina Girsang, Faculty of Pharmacy, Universitas Sumatera Utara,Medan, Indonesia

Faculty of Pharmacy, Universitas Sumatera Utara,Medan, Indonesia

Julia Reveny, Faculty of Pharmacy, Universitas Sumatera Utara,Medan, Indonesia

Faculty of Pharmacy, Universitas Sumatera Utara,Medan, Indonesia

Marline Nainggolan, Faculty of Pharmacy, Universitas Sumatera Utara,Medan, Indonesia

Faculty of Pharmacy, Universitas Sumatera Utara,Medan, Indonesia

References

1. Kementerian Kelautan dan Perikanan (KKP). 2015. Kelautan dan Perikanan dalam Angka 2015. Pusat data statistik dan informasi Sekretariat Jenderal Kementerian Kelautan dan Perikanan, Jakarta.
2. Sathivel, S., Yin, H., Prinyawiwatkul, W., King, J.M. dan Xu, Z. 2002. Economical methods to extract and purify Catfi sh oil. Published Article in the Louisiana Agiculture, LSU AgCenter, Department of Food Science, Baton Rouge La.
3. Zuta, C.P., Simpson, B.K., Chan, H.M. dan Philips, L. Concentrating PUFA from Mackerel processing waste. J Am Oil Chem. Soc.2003; 80:933-936.
4. Singh, P., Benjakul, S., Maqsood, S., & Kishimura, H. (2011). Isolation and characterisation of collagen extracted from the skin of striped catfish (Pangasianodon hypophthalmus). Food Chem.2011; 124:97-105.
5. Standar Nasional Indonesia 2891. (1992). Analisa kadar abu. Jakarta: Badan Standarisasi Nasional.
6. AOAC. 1995. Official Methods of Analysis of Association of Official Analytical Chemist. AOAC International. Virginia USA.
7. Muyonga JH, Cole CGB, Duodu KG.Characterisation of acids soluble collagen from skins of young and adult Nile perch (Lates niloticus). Food Chem.2004; 85:81-89.
8. AOAC (2005). Association of Official Analytical Chemist. Edisi Revisi. Edisi 18 2005. Official Methods of Analysis, Washington DC.
9. Zhou, P., & Regenstein, J.M. Effects of alkaline and acid pretreatments on alaska pollock skin gelatin extraction. J Food Sci, 2005; 70(6):C392-C396.
10. Jaswir I, Monsur HA, Salleh HM. Nano-structural analysis of fish collagen extracts for new process development. African Journal of Biotechnology. 2011; 10(81):18847-18854.
11. Karim, A.A., & Bhat, R.,Fish gelatin: properties, challenges, and prospects as an alternative to mammalian gelatins. Food Hydrocolloid, 2009; 23:563-576.
12. Standar Nasional Indonesia 8076:2014. (2014). Kolagen Kasar dari Sisik Ikan – Syarat Mutu dan Pengolahan. Jakarta: Badan Standarisasi Nasional.
13. Jamilah, B., Hartina, M.U., Hashim, D.M., & Sazili, A.Q. (2013). Properties of collagen from barramundi (Lates calcarifer) skin. International Food Research Journal.2013; 20(2):835-842.
14. Ariesta, C. (2014). Ekstraksi dan karakterisasi kolagen dari kulit ikan cobia (Rachycentron canadum) (skripsi). Bogor: Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, IPB.
15. Potaros, T., Raksakulthai, N., Runglerdkreangkrai, J., & Worawattanamateekul, W.Characteristics of collagen from nile tilapia (Oreochromis niloticus) skin isolated by two different methods. Kasetsart Journal2009; 43:584-593.
16. Standar Nasional Indonesia 7387-2009. (2009). Batas maksimum cemaran logam berat dalam pangan. Jakarta: Badan Standarisasi Nasional.
17. Peng, Y., Glattauer, V., Werkmeister, J.A., & Ramshaw, J.A.M. Evaluation for collagen products for cosmetic application. J Cosmestic Sci.2004; 55, 327-341.
18. Hartati I., & Kurniasari, L. Kajian produksi kolagen dari limbah sisik ikan secara ekstrasi enzimatis. Momentum.2010; 6 (1), 33-35.
19. Haris, M.A. (2008). Pemanfaatan limbah tulang ikan nila (Oreochromis niloticus) sebagai gelatin dan pengaruh lama penyimpanan pada suhu ruang (skripsi). Bogor: Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, IPB.
20. Friess W. Collagen – biomaterial for drug delivery. Eur J Pharm Biopharm.1998; 45:113-136.
21. Nalinanon S, Benjakul S, Visessanguan W, Kishimura H. Use of pepsin for collagen extraction from the skin of bigeye snapper (Priacanthus tayenus). Food Chemistry.2011; 104(2): 593–601.
22. Nagarajan, M., Benjakul, S., Prodptan, T., Songtipya, P dan Kishimura, H.Characteristic and functional properties of gelatin from splendid squid (Loliga formasana) skin as affected by extraction temperature. Food Hydrocolloid,2012; 20:389-397
23. Coates, J. (2000). Interpretation of infrared spectra, a practical approach. Di dalam: Meyers RA, editor. Encyclopedia of Analytical Chemistry. Chichester: John Wiley & Sons Ltd.
24. Kong, J., & Yu, S. Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta bioch bioph sin,2007; 39 (8), 549-559.
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How to Cite
Girsang, V., Reveny, J., & Nainggolan, M. (2020). ISOLATION AND CHARACTERIZATION COLLAGEN OF PATIN FISH SKIN (Pangasius Sp.). Asian Journal of Pharmaceutical Research and Development, 8(1), 47-51. https://doi.org/https://doi.org/10.22270/ajprd.v8i1.661