Phytochemicals Analysis and Cytotoxicity Activity of Ethanol Extract of Litseacubeba Lour.Heartwood

  • Fujiko M Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia,
  • Dalimunthe A Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia,
  • Masfria M Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia

Abstract

 


Objective: The purpose of this study was to determine the chemical compounds whichcontained in the ethanol extract and cytotoxic activity ethanol extract of Litseacubeba heartwood induced in T47D cells.


Methods: The ethanol extract was extracted by maceration using ethanol 96% solvent. Cytotoxic activity was determined with MTT method and the IC50analyzed using SPSS 23.


Results: Phytochemicals screening were showed that the ethanol extract of Litseacubeba heartwood contained steroids/triterpenoids, glycosides, alkaloids, flavonoids, saponins and tannins. The IC50of ethanol extract of Litseacubeba heartwood were 349,57 ±0,35μg/ml in T47D cells.


Conclusions: Ethanol extract of Litseacubeba heartwood has activity as an anticancer to T47D cells breast cancer agents.


 

Keywords: heartwood, Litseacubeba Lour, T47D cell, cytotoxicity, phytochemicals.

Downloads

Download data is not yet available.

Author Biographies

Fujiko M, Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia,

Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia,

Dalimunthe A, Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia,

Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia,

Masfria M, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155,  Indonesia

References

1. Dalimunthe, A., Achmad, S., and Satria, D. (2016). Phenolic, flavonoid content and antioxidant activities of ethylacetate extract of Litsea cubeba (Lour.) Pers. Heartwoods. Der Pharma Chemica.2016; 8: 466-468.
2. Trisonthi P, Sato A, Nishiwaki H, Tamura H. A new diterpene from Litseacubeba fruits: structure elucidation and capability to induce apoptosis in HeLa cells. Molecules. 2014 May 23; 19(5):6838-50.
3. Atta-ur-Rahman. (2009). Bisbenzylisoquinoline alkaloids from Cocculus pendulus. Natural Product Research.2009; 23(14):1265-1273.
4. Torre LA, et al. (2015.) Global cancer statistics, CA Cancer J Clin. 2015; 1:65(2):87– 108.
5. Siegel RL, Miller KD, and Jemal A. Cancer statistics, CA Cancer J Clin.2017; 1:67(1):7–30.
6. Conze, D. et al., Autocrine Production of Interleukin 6 Causes Multi Drug Resistance In Breast Cancer Cells. Cancer Research. 2001; 61. 8851–8858.
7. Shapiro, G.I., and Harper, J.W.,Anticancer Drug Targets: Cell Cycle and Checkpoint Control, J. Clin. Invest., 1999; 104, 1645-1653.
8. Fisher, D.E., Apoptosis in Cancer Therapy: Crossing The Threshold. Cell. 1994; 78. 539-542.
9. Kitagawa, S.Inhibitory Effect of Polyphenols on P-Glycoprotein-Mediated Transport, Biol. Pharm Bull. 2006; 29, 1-6.
10. Desai AG, et al. Medicinal plants and cancer chemoprevention. Curr Drug Metab.2008 ; 9(7):581–591.
11. Dalimunthe, A., Poppy, A., Jansen, S., Siti, F., and Denny, S., (2018). Antioxidant Activity of Alkaloid Compounds from Litsea cubeba Lour. Oriental Journal of Chemistry, 2018; 34: 1149-1152.
12. D. Satria, J. Silalahi, G. Haro, S. Ilyas, P. A. Z. Hasibuan. Chemical Analysis and Cytotoxic Activity of N-Hexane Fraction of ZanthoxylumacanthopodiumDC. Fruits. Rasayan J Chem. 2019; 12(2):803-808.
13. Hutagaol, Sherly.,Rosidah., Masfria., and Denny, S., Combination effect of ethylacetate extract leaves of MoringaoleiferaL. and Doxorubicin against MCF-7 cell lines. Journal of Innovations in Phramaceutical and Biological Sciences, 2018; 5(3) : 42-46.
14. Dalimunthe, A., Poppy, A., Jansen, S., and Denny, S., (2018). Aktivitas Sitotoksik Fraksi Alkaloid Kulit Batang dan Buah Attarasa (Litsea cubeba Lour.) terhadap Sel Kanker Payudara T47D. TM Conference Series 2018; 01 :052–055.
15. Tussannti, Iin., Andrew Johan and Kisdjamiatun, 2014, Sitotoksisitas in vitro ekstraketanolikbuahparijoto (Medinillaspeciosa, reinw.ex bl.) terhadapselkankerpayudara T47D, Jurnal Gizi Indonesia, 2014; 2(2):1858-4942.
16. Suffness, M, Pezzuto, JM, Assays Related to Cancer Drug Discovery. In Methods in Plant Biochemistry: Assays for Bioactivity; Hostettmann, K, Ed.; Academic Press: London, UK, 1990; 6, 71–133.
Statistics
9 Views | 10 Downloads
How to Cite
M, F., A, D., & M, M. (2020). Phytochemicals Analysis and Cytotoxicity Activity of Ethanol Extract of Litseacubeba Lour.Heartwood. Asian Journal of Pharmaceutical Research and Development, 8(1), 14-17. https://doi.org/https://doi.org/10.22270/ajprd.v8i1.643