Inhibitory AKT2 And Vegr2 Genes Expression of Avicennia Marina Using RT-PCR On Widr Cells
Objectives: To evaluate the activities the anticancer of n-hexane extract of Avicennia marina leaves on WiDr cells in down-regulated of expression of Akt2 and VEGFR2 genes.
Methods: Avicennia marina leaves were dried and extracted with n-hexane, analyzed the down-regulated Akt 2 and VEGFR2 expression which was determined the Reverse Transcription Polymerase Chain Reaction (RT-PCR) method.
Results:N-hexane extract of Avicennia marina, in which there were down regulated expression Akt 2 and VEGFR2 of 0.43 and 0.50 WiDr cells. N-hexane extract of mangrove leaves has cancer chemoprevention activity with down-regulated on WiDr cells.
Conclusions: :N-hexane extract of Avicennia marina leaves had anticancer activity with down-regulated on WiDr cells, suggest that significantly inhibit the expression of Akt2 and VEGFR2 genes.
2. Kurnijasanti, R., Hamid, S.I., and Rahmawati, K. Efek Sitotoksik In Vitro dari Ekstrak Buah Mahkota Dewa (Phaleria macrocarpa) Terhadap Kultur Sel Kanker Mieloma.Media Eksakta. 2008;7(1): 48–54.
3. Akter, R, Uddin, SJ, Grice, I.D and Tiralongo, E. Cytotoxic activity screening of Bangladeshi medicinal plant extracts. J Nat Med. 2013: 1-7.
4. Niranjana, R., Gayathri, R., MoSN, Sugawara, T., Hirata, T., Miyashita, K., and Ganesan, P. Carotenoids modulate the hallmar Control of cancer cells. Funct J Foods. 2015;18:968-985.
5. Sari, P.D, Basyuni, M., and Hasibuan, PAZ. Inhibition Polyisoprenoids from Nypa fruticans leaves on cyclooxygenase two expressions of Widr colon cancer cells. Asians Journal of Pharmaceutical and Clinical Research. 2018; 11:154-157..
6. Illian, ND, Basyuni, M., and Hasibuan, PAZ. Polyisoprenoids From A. marina and Avicennia lanata Widr cells inhibit proliferation. Pharmacognosy Magazine. 2018; 14:513-518.
7. Brugge J, Hung M,C and Mills G,B. A new mutational Aktivation in the PI3K pathway. Cancer Cell. 2007.12:104 –107.
8. Testa, J.R, and Bellacosa, A. AKT plays a central role in tumorigenesis. PNAS 98. 2001:10983 – 10985.
9. Bellacosa, A. Molecular alterations of the AKT2 oncogene in ovarian and breast carcinomas. Int J Cancer. 1995. 64:280 –285.
10. Rychahou, P.G, Jackson, L.N, Silva, S.R, Rajaraman. S, and Evers, B.M .Targeted molecular therapy of the PI3K pathway: Therapeutic significance of PI3K subunit targeting in colorectal carcinoma. Ann Surg . 2006; 243:833– 842, discussion 843– 834.
11. Byrne, A.M, Bouchier-Hayes, D.J, and Harmey, J.H. Angiogenic and cell survival functions of vascular endothelial growth factor (VEGF). J Cell Mol Med. 2005;9: 777–794.
12. Strizzi, L, Catalano, A, Vianale, G, Orecchia, S, and Casalini, A. Vascular endothelial growth factor is an autocrine growth factor in human malignant mesothelioma. J Pathol. 2001; 193: 468–475.
13. Basyuni, M, Sagami, H, Baba, S and Oku, H. Distribution, occurrence and cluster analysis of new polyprenyl acetones and other polyisoprenoids from North Sumatran mangroves. Dendrobiology. 2017; 78:18–31.
14. Cancer Chemoprevention Research Centre. The protocol of apoptic test double staining method. Yogyakarta. 2013;1-4.
15. Zhang, Y, Guan, X.Y, Dong, B, Zhao, M, Wu, J.H, and Tian, X.Y. Expression of MMP-9 and WAVE3 in colorectal cancer and its relationship to clinicopathological features. J Cancer Res Clin Oncol. 2011; 138:2035-2044.
16. Kamal, Reddy, K.S, Khan, M.N, Shetti, R.V,Ramaiah, MJ, and Pushpavalli, S.N. Synthesis, DNA-binding ability and anticancer activity of benzothiazole/benzoxazole–pyrrolo [2,1-c][1,4] benzodiazepine conjugates. Bioorg Med Chem. 2010; 18(13):4747–61.
17. Liren. Lemon Pepper Fruit Extract (Zanthoxylum acanthopodium DC.) Suppresses the Expression of Inflammatory Mediators in Lipopoly saccharide-Induced Macrophages In-Vitro. American Journal of Biochemistry and Biotechnology. 2011;7(4):190-5. https://doi.org/10.3844/ajbbsp.2011.190.195.
18. Yoon, D., Wang, Y., Stapleford, K., Wiesmuller, L and Chen, J. P53 Inhibits strand exchange and replication fork regression promoted by Human Rad 51. Journal of Molecular Biology. 2004. 336(3). 639- 654.
19. Radhakrishnan, P., Baraneedharan, U.,Veluchamy, S, Dhandapani, M, Pinto, D.D, and Thiyagarajan, S. Inhibition of Rapamycin-Induced AKT Activation Elicits Differential Antitumor Response in Head and Neck Cancers. Cancer Research. 2013; 73(3):1118-27.
20. Khursheed, A. Plant-based natural compounds and herbal extracts as promising apoptotic agents: their implications for cancer prevention and treatment. Advances in Biomedicine and Pharmacy. 2016; 03(04):225-48.
21. Momota, H, Nerio, E, and Holland, E.C. Perifosine inhibits multiple signaling pathways in glial progenitors and cooperates with temozolomide to arrest cell proliferation in gliomas in vivo. Cancer Res. 2005. 65:7429 –7435.
22. Giatromanolaki, A., Sivridis, E., and Koukourakis, M.I. Angiogenesis in colorectal cancer: Prognostic and therapeutic implications. Am J Clin Oncol. 2006; 29:408–17.
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