Anti-Inflammation Activity of Virgin Coconut Oil In- Vitro Against Raw Cells 264.7

  • Amin M Department of Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Silalahi J Department of Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Harahap U Departemen of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia;
  • Satria D Departemen of Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

Abstract

Objective:This study aimsto determine the anti-inflammation activity of pure coconut oil on Raw Cell 264.7 induced by LPSMethods: The anti-inflammation activity test for Virgin Coconut Oil (VCO) was carried out by testing the expression of TNF-α, IL-6, IL-1β, iNOS, and COX-2 genes on RAW 264.7 cells induced by LPS by the method Reverse Transcription-Polymerase Chain Reaction (RT-PCR).Results: The test results of iNOS, TNF-α, IL-6, IL-1β, and COX-2 gene expression from VCO on RAW 264.7 cells induced by lipopolysaccharide (LPS) decreased the value of VCO density. In iNOS expression, VCO density values ​​(0,90±0,015) showed significantly different effects with positive and negative controls p<0,05, VCO IL-1β density values ​​(2,47±0,010) showed different effects. significant with normal control, positive control and negative control p<0,05, TNF-α density value on VCO (0,91±0,010) showed significantly different effects from positive control and negative control p<0,05, then the value IL-6 density at VCO (1,23 ± 0,015) showed significantly different effects from normal and positive controls p<0,05, and COX-2 density values ​​at VCO (1,02±0,015) showed significantly different effects with normal and positive controls p<0,05.Conclusion: Based on the results of this study VCO on RAW 264.7 cells induced by LPS can inhibit the expression of TNF-α, IL-6, IL-1β, iNOS, and COX-2 genes so that VCO effectively has anti-inflammatory activity. 

Keywords: VCO, Antiinflammation, RAW 264.7 Cells, Gene Expression, Lipopolysaccharides

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Author Biographies

Amin M, Department of Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

Department of Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

Silalahi J, Department of Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

Department of Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

Harahap U, Departemen of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia;

Departemen of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia;

Satria D, Departemen of Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

Departemen of Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

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How to Cite
M, A., J, S., U, H., & D, S. (2020). Anti-Inflammation Activity of Virgin Coconut Oil In- Vitro Against Raw Cells 264.7. Asian Journal of Pharmaceutical Research and Development, 8(1), 55-58. https://doi.org/https://doi.org/10.22270/ajprd.v8i1.633