n-Hexane Extract of Mangrove Leaves Effect on p21 and Akt 2 Gene Expressions of WiDr Cells

  • Istiqomah M A Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia
  • Hasibuan P. A. Z Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia
  • Basyuni M Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Medan, 20155, Indonesia

Abstract

Objectives: The study aims to examine the anticancer effect of polyisoprenoid of Nypa fruticans, Ceriops tagal, and Rhizophora mucronata leaves in WiDr cells by evaluating the regulation of p21 and Akt 2 gene.


Design: Nypa fruticans, Ceriops tagal, and Rhizophora mucronata leaves were dried and extracted with n-hexane, analyzed the increase or decrease in regulation of p21 gene and Akt 2 expression which was determined the Reverse Transcription Polymerase Chain Reaction (RT-PCR) method.


Interventions: The variable that was intervened in this study was the 3 sample mangrove leaves.


Main outcome measure: The main measurement results in this study were to study n-hexane extracts of mangrove leaves able to suppress the expression of p21 and Akt 2 genes so that cancer cell growth is inhibited.


Results: n-hexane extract of Ceriops tagal leaves was more effective than Nypa fruticans and Rhizophora mucronata, in which there was up-regulated (p21) of 1.19 and down regulated (Akt 2) of 0.78 on colon cancer cells (WiDr). N-hexane extract of mangrove leaves has cancer chemoprevention activity with up-regulated and down-regulated on WiDr cells, in which the sample was more effective than n-hexane extract of Ceriops tagal leaves.


Conclusion: N-hexane extract of mangrove leaves had cancer chemoprevention activity with up-regulated and down-regulated on WiDr cells, in which the sample was more effective than n-hexane extract of Ceriops tagal leaves.


 


 

Keywords: Rhizophora mucronata, Nypa fruticans, leaves, Ceriops tagal, daun

Downloads

Download data is not yet available.

Author Biographies

Istiqomah M A, Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia

Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia

Hasibuan P. A. Z, Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia

Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia

Basyuni M, Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Medan, 20155, Indonesia

Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Medan, 20155, Indonesia

References

1. Kawada M, Uehara Y, Mizuno S, Yamori T, Tsuruo T. Upregulation of p27 Kip1 correlates inversely with anchorage independent growth of human cancer cell lines. Jpn J Cancer Res. 1998; 89(2):110-115.
2. Sarioglu S, Sokmen S, Terzi C. Relationship of Angiogenesis and p53 Protein Expression in Colorectal Carcinomas. Colorect Dis. 2001; 3(1):38–41.
3. Toyoshima H, Hunter T. p27, a novel inhibitor of G1 cyclin–Cdk protein kinase activity, is related to p21. Cell. 1994; 78(1):67–74.
4. Harper JW, Adami GR, Wei N. The p21 Cdk-interacting Protein Cip1 is Potent Inhibitor of G1 cyclin-dependent kinases. Cell. 1993; 75(4):805–816.
5. Xiong Y, Hannon GJ, Zhang H. p21 is A Universal Inhibitor of Cyclin Kinases. Nature. 1993; 366:701–704.
6. Polyak K, Lee MH. Erdjument–Bromage. Cloning of p27 Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals. Cell. 1994; 78:59–66.
7. El-Deiry WS, Harper JW. WAF1/CIP1 is Induced in p53-mediated G1 Arrest and Apoptosis. Cancer Res. 1994; 54:1169–1174.
8. Barbareschi M, Caffo O, Doglioni C. p21 WAF1 Immunohistochemical Expression in Breast Carcinoma: correlation with clinicopathological data, estrogen receptor status, MIB1 expression, p53 gene and protein alterations and relapse-free survival. Br J Cancer. 1996; 74:208–215.
9. Baker SJ, Markowitz S, Fearon SR, Willson JK, Vogelstein B. Suppression of Human Colorectal Carcinoma Cell Growth By Wild Type P53. Science.1990; 249:912−915.
10. Purnobasuki H. Potensi Mangrove Sebagai Tanaman Obat Prospect of Mangrove as Herbal Medicine. Surabaya. UNAIR. 2004
11. Illian DN, Basyuni M, Wati R, Hasibuan PAZ. Polyisoprenoids from Avicennia marina and Avicennia lanata Inhibit WiDr Cells Proliferation.Phcog Mag. 2018; 14(58):516-518(2018),
12. Sari DP, Basyuni M, Hasibuan PAZ, Wati R. The Inhibition of Polyisoprenoids from Nypa fruticans Leaves on Cyclooxygenase 2 Expression of WiDr Colon Cancer Cells. Asian J Pharm Clin Res. 2018; 11(8):156.
13. Illian DN, Hasibuan PAZ, Sumardi S, Nuryawan A, Basyuni M. Anticancer Activity of polyisoprenoids from Avicennia alba Blume. in WiDr Cells. Iran J Pharm Res. 2019; 18(3):1477–1487.

14. Basyuni M, Sagami H, Baba S, Oku H. Distribution and occurrence of new polyprenyl acetone and other polyisoprenoids in Indonesian mangroves. Dendrobiology. 2017; 78:18–31.
15. Nomani A, Fouladdel S, Haririan I, Rahimnia R, Ruponen M, Gazori T, et.al. Poly (amidoamine) dendrimer silences the expression of epidermal growth factor receptor and p53 gene in vitro. Afr J Pharm Pharmacol. 2012; 6(8):530–537.
16. Dong M, Yang G, Liu H, Liu X, Lin S, Sun S, et. al. Aged black garlic extract inhibits HT29 colon cancer cell growth via the PI3K/Akt signaling pathway. Biomed Rep. 2014; 2(2):250–254.
17. Matsumoto K, Arao T, Tanaka K, Kaneda H, Kudo K, Fujita Y, et. al. mTOR signal and hypoxia-inducible factor-1α regulate CD133 expression in cancer cells. Cancer Res. 2009; 69(18):7160-64.
18. Wang H, Duan L, Zou Z, Li H, Yuan S, Chen X, et. al. Activation of the PI3K/Akt/mTOR/p70S6K pathway is involved in S100A4-induced viability and migration in colorectal cancer cells. Int J Med Sci. 2014; 11(8):841.
19. Hassan ZK, Elamin MH, Daghestani MH, Omer SA, Al-Olayan EM, Elobeid MA, et. al. Oleuropein induces anti-metastatic effects in breast cancer. Asian Pac J Cancer Prev. 2012; 13(9):4555–4559.
20. Zhang Y, Guan XY, Dong B, Zhao M, Wu JH, Tian XY, et. al. Expression of MMP-9 and WAVE3 in colorectal cancer and its relationship to clinicopathological features. J Cancer Res Clin Oncol. 2011; 138:2035–2044.
21. Kamal, Reddy KS, Khan MN, Shetti RV, Ramaiah MJ, Pushpavalli SN, et. al. Synthesis, DNA-binding ability and anticancer activity of benzothiazole/benzoxazole–pyrrolo [2, 1-c][1, 4] benzodiazepine conjugates. Bioorg Med Chem. 2010; 18(13):4747–61.
22. Kawauchi S, Goto Y, Ihara K, Furuya T, Oga A, Tsuneyoshi M, et. al. Survival Analysis with p27 Expression and Apoptosis Appears to Estimate the Prognosis of Patients with Synovial Sarcoma More Accurately. Cancer. 2002; 94(10):2712-2718.
23. Brissett AE, Olsen KD, Kasperbauer JL, Lewis JE, Goellner JR, Spotts BE, et. al. Merkel cell carcinoma of the head and neck: a retrospective case series. Head Neck. 2002; 24(11):982-988.
24. Ahmed NU, Ueda M, Ichihashi M. p21WAF1/CIP1 Expression in Non-Melanoma Skin Tumors. J Cutan Pathol. 1997; 24(4):223-227.
25. Geisler GE, Geisler JP, Miller GA, Geisler MJ, Wiemann MC, Zhou Z, et. al. p21 and p53 in ovarian carcinoma: their combined staining is more valuable than either alone. Cancer. 2001; 92(4):781-786.
26. Stefanaki K, Rontogiannis D, Vamvouka C, Bolioti S, Chaniotis V, Sotsiou F, et al. Immunohistochemical detection of BCl 2, p53, mdm2 and p21/waf1 proteins in small-cell lung carcinomas. Anticancer Res. 1998; 18(3A):1689-1695.
27. Barak Y, Juven T, Haffner R, Oren M. MDM 2 Expression is Induced By Wild Type p53 Activity. Embo. J. 1993; 12:461−468.
28. Bischoff JR, Kirn DH, Williams A, Heise C, Horn S, Muna M, et. al. An Adenovirus Mutant That Replicates Selectively in p53 Deficient Human Tumor Cells. Sciences. 1996; 274:373-376.
Statistics
79 Views | 112 Downloads
How to Cite
A, I., Z, H., & M, B. (2019). n-Hexane Extract of Mangrove Leaves Effect on p21 and Akt 2 Gene Expressions of WiDr Cells. Asian Journal of Pharmaceutical Research and Development, 7(6), 20-24. https://doi.org/https://doi.org/10.22270/ajprd.v7i6.624