Antiinflammatory Activity of Pagoda Flower (Clerodendrum Paniculatum L.) Ethanol Extract Using Paw Edema Method

  • Ihsanul Hafiz Faculty of Pharmacy and Health, Institut Kesehatan Helvetia,Kapten Sumarsono Street 107, Medan, Sumatera Utara, Indonesia
  • Mandike Ginting Faculty of Pharmacy and Health, Institut Kesehatan Helvetia,Kapten Sumarsono Street 107, Medan, Sumatera Utara, Indonesia

Abstract

Objectives: The purpose of this study was to determine antiinflammatory activity of pagoda flower (Clerodendrum paniculatum L.) ethanolic extract


Design: This study uses an experimental laboratory design. This research uses paw edema method by inducing carrageenin in the legs of male white rats as an induction of inflammation.


Interventions: The sample used was pagoda flower ethanol extract in various dosages of 25, 50 and 100 mg / kg. As a comparison, acetosal dose 33 mg / kg was used. Na CMC suspension was used as a negative control.


Main outcome measure: The results in this study are the difference in the volume of edema volume from rat feet per unit time. The measurement of the rat's leg volume was measured at 30, 60, 120, 180, 240 and 300 minutes.


Conclusion: Pagoda flower ethanol extract does not have good anti-inflammatory activity. there were no significant differences between groups except at dose 100 and positive control at minute 300 of negative control.


 


 

Keywords: antiinflammatory activity, Clerodendrum paniculatum L., paw edema

Downloads

Download data is not yet available.

Author Biographies

Ihsanul Hafiz, Faculty of Pharmacy and Health, Institut Kesehatan Helvetia,Kapten Sumarsono Street 107, Medan, Sumatera Utara, Indonesia

Faculty of Pharmacy and Health, Institut Kesehatan Helvetia,Kapten Sumarsono Street 107, Medan, Sumatera Utara, Indonesia

Mandike Ginting, Faculty of Pharmacy and Health, Institut Kesehatan Helvetia,Kapten Sumarsono Street 107, Medan, Sumatera Utara, Indonesia

Faculty of Pharmacy and Health, Institut Kesehatan Helvetia,Kapten Sumarsono Street 107, Medan, Sumatera Utara, Indonesia

References

1. Hafiz I, Rosidah, Silalahi J. Antioxidant and anti-inflammatory activity of pagoda leaves (clerodendrum paniculatum l.) ethanolic extract in white male rats (Rattus novergicus). Int J PharmTech Res. 2016; 9(5):165-170.
2. Matsushima K, Terashima Y, Toda E, Shand F, Ueha S. Chemokines in inflammatory and immune diseases. Inflamm Regen. 2011;31(1):11–22.
3. Yankanchi SR, Koli SA. Anti-inflammatory and Analgesic activity of mature leaves methanol extract of Clerodendrum inerme L.(Gaertn). J Pharm Sci Res. 2010; 2(11):782.
4. Bhangare NK, Pansare TA, Ghongane BB, Nesari TM. Screening for anti-inflammatory and anti-allergic activity of Bharangi {Clerodendrum serrratum (Linn.) moon} in animals. Int J Pharma Bio Sci. 2012; 3(4): 245–54.
5. Parekar RR, Dash KK, Marathe PA, Apte AA, Rege NN. Evaluation of anti-inflammatory activity of root bark of Clerodendrum phlomidis in experimental models of inflammation. International Journal of Applied Biology and Pharmaceutical Technology. 2012; 3(3):55-60.
6. Chandrashekar R, Rao SN. Chronic anti-inflammatory activity of ethanolic extract of leaves of Clerodendrum viscosum by carrageenin induced paw oedema in Wistar albino rats. Br J Pharm Res. 2013 ;3(4):579.
7. Hafiz I. The Phytochemical Screning and Assesment of Bioactivity of Pagoda Flower (Clerodendrum paniculatum L.) Using Brine Shrimp (Artemia salina) Lethality Assay. Asian J Pharm Res Dev. 2019; 7(3):10–3.
8. Azwanida NN. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants. 2015; 4(196):412–2167.
9. Lemaire C, Plenevaux A, Aerts J, Del Fiore G, Brihaye C, Le Bars D, et al. Solid phase extraction—an alternative to the use of rotary evaporators for solvent removal in the rapid formulation of PET radiopharmaceuticals. J Label Compd Radiopharm Off J Int Isot Soc. 1999; 42(1):63–75.
10. Carvalho GD, Masseno APB, Zanini MS, Zanini SF, Porfirio LC, Machado JP, et al. Clinical evaluation of laboratory rats (Rattus novergicus Wistar strain): sanitary biological and physiological parameters. Rev Ceres. 2009; 56(1):51–7.
11. Fraser D. Animal ethics and animal welfare science: bridging the two cultures. Appl Anim Behav Sci. 1999; 65(3):171–89.
12. Phuneerub P, Limpanasithikul W, Palanuvej C, Ruangrungsi N. In vitro anti-inflammatory, mutagenic and antimutagenic activities of ethanolic extract of Clerodendrum paniculatum root. J Adv Pharm Technol Res. 2015; 6(2):48.
13. Winter CA, Risley EA, Nuss GW. Carrageenin-induced edema in hind paw of the rat as an assay for antiinflammatory drugs. Proc Soc Exp Biol Med. 1962; 111(3):544–7.
Statistics
83 Views | 110 Downloads
How to Cite
Hafiz, I., & Ginting, M. (2019). Antiinflammatory Activity of Pagoda Flower (Clerodendrum Paniculatum L.) Ethanol Extract Using Paw Edema Method. Asian Journal of Pharmaceutical Research and Development, 7(6), 43-45. https://doi.org/https://doi.org/10.22270/ajprd.v7i6.622