Assessment of In-Vivo Antioxidant Potential of Hydro-Alcoholic Extract and Ethyl Acetate Fraction of Aerva Javanica Linn. Flowering Tops

Authors

  • Alok Khunteta LBS College of Pharmacy, Jaipur-302004, Rajasthan, India
  • Surendra K Swarnkar LBS College of Pharmacy, Jaipur-302004, Rajasthan, India
  • Manish Kumar Gupta School of Pharmaceutical Sciences, Jaipur National University, Jaipur-302017, Rajasthan, India
  • Aruna Swarnkar SMS Medical College, Jaipur-302004, Rajasthan, India
  • Swapnil Sharma Department of Pharmacy, Banasthali Vidyapith, Banasthali, Newai, Rajasthan, India
  • Sarvesh Paliwal Department of Pharmacy, Banasthali Vidyapith, Banasthali, Newai, Rajasthan, India

DOI:

https://doi.org/10.22270/ajprd.v7i6.614

Keywords:

Patharphori, Tris, OECD, TBARS, Malondialdehyde, Silymarin

Abstract

Aerva javanica (Amaranthaceae) is a grey coloured woolly perennial tomentose shrub. Its traditional and folklore usage motivates further investigation on its pharmacognostic parameters and pharmacological potential. Hydro-alcoholic extract (AJCE) was prepared from flowering tops of A. javanica. In order to work further on activity guided fractions, ethyl acetate (AJEAF) fraction was prepared.  Therefore, in order to establish its antioxidant potential, in-vivo effect on LPO, GSH, SOD and catalase activity was determined. For comparison, silymarin and Centella asiatica extract (CAE) were used as standard antioxidant compound/extract. Lipid peroxidation in term of MDA content expressed as nM/mg, which was 82.18 and 67.39 for AJCE with increasing doses of complete hydro-alcoholic extract (AJCE represented as AJCE-1 and AJCE-2) and 51.65 for AJEAF in contrast to 40.64 nM/mg for standard silymarin and 46.81 nM/mg for standard CAE. GSH content was determined as 3.12, 3.82 and 4.56 μg/mg wet tissue in contrast to 5.59 for standard silymarin and 4.42 for standard CAE. Superoxide scavenging was expressed as SOD U/mg wet tissue, determined as 7.26, 9.16 and 9.91 U/mg wet tissue for AJCE-1 (250 mg/kg i.p. b.w), AJCE-2 (500 mg/kg i.p. b.w), and AJEAF respectively in comparison to silymarin (10.11) and CAE (46.81 U/mg wet tissue). Catalase activity expressed as μM of H2O2 decomposed / min / mg wet tissue was determined as 0.61, 0.72 and 0.78 repectively for AJCE-1 (250 mg/kg i.p. b.w), AJCE-2 (500 mg/kg i.p. b.w), and AJEAF. Results indicated the SOD values and total antioxidant power of DEE and EAF fractions even better than standard ascorbic acid which expressed the prospective potential of fractions (DEE and EAF) against metabolic disorders.

 

Downloads

Download data is not yet available.

Author Biographies

Alok Khunteta, LBS College of Pharmacy, Jaipur-302004, Rajasthan, India

LBS College of Pharmacy, Jaipur-302004, Rajasthan, India

Surendra K Swarnkar, LBS College of Pharmacy, Jaipur-302004, Rajasthan, India

LBS College of Pharmacy, Jaipur-302004, Rajasthan, India

Manish Kumar Gupta, School of Pharmaceutical Sciences, Jaipur National University, Jaipur-302017, Rajasthan, India

School of Pharmaceutical Sciences, Jaipur National University, Jaipur-302017, Rajasthan, India

Aruna Swarnkar, SMS Medical College, Jaipur-302004, Rajasthan, India

SMS Medical College, Jaipur-302004, Rajasthan, India

Swapnil Sharma, Department of Pharmacy, Banasthali Vidyapith, Banasthali, Newai, Rajasthan, India

Department of Pharmacy, Banasthali Vidyapith, Banasthali, Newai, Rajasthan, India

Sarvesh Paliwal, Department of Pharmacy, Banasthali Vidyapith, Banasthali, Newai, Rajasthan, India

Department of Pharmacy, Banasthali Vidyapith, Banasthali, Newai, Rajasthan, India

References

1. Nair S, Nair M, Nair D, Juliet S, Padinchareveetil S, Samraj S, et al. Wound Healing , Anti Inflammatory Activity and Toxicological Studies of Leea Asiatica (L.) Ridsdale. Int J Biol Pharm Res. 2014;5(9):745–9.
2. Kirtikar K, Basu B. Indian Medicinal plants Plates. 1918; 4:791
3. Chopra R, Nayar S, Chopra I. Glossary of Indian Medicinal Plants. CSIR,(New Delhi, India). 1956; 186–7.
4. Zaveri M, Movaliya V, Setty MM. Pharmacognostical Studies on Aerial Parts of Aerva javanica. Adv Res Pharm Biol. 2012; 2(III):250–3.
5. Soliman MA. Cytogenetical studies on Aerva javanica ( Amaranthaceae ). Flora Mediterr. 2006; (16):333–9.
6. Khunteta A, Swarnkar S, Gupta M, Swarnkar A, Jain P, Paliwal S. Assessment of In-Vitro Antioxidant Potential of Ethyl Acetate Fraction of Hydroalcoholic Extract of Aerva Javanica Linn. Flowering Tops. Asian J Pharm Res Dev, 2019; 7(5):133–6.
7. Swarnkar S, Parnami A, Barotia K, Kumar Gupta M, Sharma D, Paliwal S. Phytochemical standardization of extracts of Aerva javanica Linn. flowering tops through determination of total phenolic, flavonoid and flavonol content. Asian J Biochem Pharm Res, 2019;(SI):12–6.
8. Swarnkar SK, Dwivedi D, Sharma R, Gupta D, Kaushik K, Gupta P. Assessment of superoxide scavenging and total antioxidant potential of hydroalcoholic extract of Aerva javanica Linn. flowering tops. Asian J Biochem Pharm Res 2019;(SI):22–6.
9. Manda H, Rao BK, Yashwant, Kutty NG, Swarnkar A, Swarnkar SK. Antioxidant , Anti-Inflammatory and Antipyretic Activities of Ethyl Acetate Fraction of Ethanolic Extract of Schrebera swietenioides roxb. Root. Int J Toxicol Pharmacol Res. 2009; 1(1):7–11.
10. Swarnkar S, Jain Y, Kumawat M, Khunteta A, Paliwal S. Exploration of autonomic involvement in mechanism of antinociceptive activity of flowering top extract of Aerva javanica. Asian J Biochem Pharm Res. 2019; (SI):33–6.
11. OECD/OCDE. OECD guideline for testing of chemicals: Acute oral toxicity. 2001; 12.
12. Laxane SN, Swarnkar SK, Zanwar SB, Manjunath Setty M. Anti-inflammatory studies of the alcoholic extract of Zornia gibbosa. Pharmacologyonline. 2011; 1:67–76.
13. Naskar S, Islam A, Mazumder UK, Saha P, Haldar PK, Gupta M. In Vitro and In Vivo Antioxidant Potential of Hydromethanolic Extract of Phoenix dactylifera Fruits. J Sci Res. 2010 ;2(1):144–57.
14. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95(2):351–8.
15. Onoja SO, Omeh YN, Ezeja MI, Chukwu MN. Evaluation of the in vitro and in vivo antioxidant potentials of Jatropha tanjorensis methanolic leaf extract. J Trop Med. 2014;6.
16. Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 1990 ;186:421–31.
17. Marklund S, Marklund G. Involvement of the Superoxide Anion Radical in the Autoxidation of Pyrogallol and a Convenient Assay for Superoxide Dismutase. Eur J Biochem. 1974; 47:469–74.
18. Sumner J, Somers G. Chem. and methods of enzymes. 2nd ed. New York; 1947. 209 p.
19. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82(1):70–7.
20. Mohamed Saleem TS, Madhusudhana Chetty S, Ramkanth S, Rajan VST, Mahesh Kumar K, Gauthaman K. Hepatoprotective herbs - A review. Int J Res Pharm Sci. 2010; 1(1):1–5.
21. Nagano K, Umeda Y, Senoh H, Gotoh K, Arito H, Yamamoto S, et al. Carcinogenicity and chronic toxicity in rats and mice exposed by inhalation to 1,2-dichloroethane for two years. J Occup Health. 2006; 48(6):424–36.
22. Zama D, Meraihi Z, Tebibel S, Benayssa W, Benayache F, Benayache S, et al. Chlorpyrifos-induced oxidative stress and tissue damage in the liver, kidney, brain and fetus in pregnant rats: The protective role of the butanolic extract of Paronychia argentea L. Indian J Pharmacol. 2007; 39(3):145.
23. Abdel-Sattar EA, Mouneir SM, Asaad GF, Abdallah HM. Protective effect of Calligonum comosum on haloperidol-induced oxidative stress in rat. Toxicol Ind Health. 2014;30(2):147–53.
24. Malomo SO, Ore A, Yakubu MT. In vitro and in vivo antioxidant activities of the aqueous extract of Celosia argentea leaves. Indian J Pharmacol. 2011; 43(3):278–85.
25. Alici E, Arabaci G. Determination of SOD, POD, PPO and CAT Enzyme Activities in Rumex obtusifolius L. Annu Res Rev Biol, 2016; 11(3):1–7.
26. Mabeau S, Baty-Julien C, Hélias AB, Chodosas O, Surbled M, Metra P, et al. Antioxidant activity of artichoke extracts and by-products. Acta Hortic. 2007; 730:491–6

Published

2019-12-15

How to Cite

Khunteta, A., Swarnkar, S. K., Gupta, M. K., Swarnkar, A., Sharma, S., & Paliwal, S. (2019). Assessment of In-Vivo Antioxidant Potential of Hydro-Alcoholic Extract and Ethyl Acetate Fraction of Aerva Javanica Linn. Flowering Tops. Asian Journal of Pharmaceutical Research and Development, 7(6), 72–78. https://doi.org/10.22270/ajprd.v7i6.614